Structure of the substrate-engaged SecA-SecY protein translocation machine

ATP-induced asymmetric pre-protein folding as a driver of protein translocation through the Sec machinery

(2019) Robin A Corey et al.   eLife

Yeast surface display platform for rapid discovery of conformationally selective nanobodies

(2018) Conor McMahon et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Penetration into membrane of amino-terminal region of SecA when associated with SecYEG in active complexes

(2018) Bahar T. Findik et al.   PROTEIN SCIENCE

Yeast surface display platform for rapid discovery of conformationally selective nanobodies

(2018) Conor McMahon et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Real-space refinement in PHENIX for cryo-EM and crystallography

(2018) Pavel V. Afonine et al.   Acta Crystallographica Section D-Structural Biology

New tools for automated high-resolution cryo-EM structure determination in RELION-3

(2018) Jasenko Zivanov et al.   eLife

The SecA protein deeply penetrates into the SecYEG channel during insertion, contacting most channel transmembrane helices and periplasmic regions

(2017) Tithi Banerjee et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

An alternate mode of oligomerization for E. coli SecA

(2017) Aliakbar Khalili Yazdi et al.   Scientific Reports

Toward a structural understanding of co-translational protein translocation

(2016) Rebecca M Voorhees et al.   CURRENT OPINION IN CELL BIOLOGY

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

Crystal structure of a substrate-engaged SecY protein-translocation channel

(2016) Long Li et al.   NATURE

Protein export through the bacterial Sec pathway

(2016) Alexandra Tsirigotaki et al.   NATURE REVIEWS MICROBIOLOGY

Two-way communication between SecY and SecA suggests a Brownian ratchet mechanism for protein translocation

(2016) William John Allen et al.   eLife

Conformational Changes of the Clamp of the Protein Translocation ATPase SecA

(2015) Yu Chen et al.   JOURNAL OF MOLECULAR BIOLOGY

Structure of the Sec61 channel opened by a signal sequence

(2015) R. M. Voorhees et al.   SCIENCE

Crystal Structures of SecYEG in Lipidic Cubic Phase Elucidate a Precise Resting and a Peptide-Bound State

(2015) Yoshiki Tanaka et al.   Cell Reports

A “Push and Slide” Mechanism Allows Sequence-Insensitive Translocation of Secretory Proteins by the SecA ATPase

(2014) Benedikt W. Bauer et al.   CELL

The Sec translocon mediated protein transport in prokaryotes and eukaryotes

(2014) Kärt Denks et al.   MOLECULAR MEMBRANE BIOLOGY

Structures of the Sec61 complex engaged in nascent peptide translocation or membrane insertion

(2014) Marko Gogala et al.   NATURE

Visualization of a polytopic membrane protein during SecY-mediated membrane insertion

(2014) Lukas Bischoff et al.   Nature Communications

Position-dependent Effects of Polylysine on Sec Protein Transport

(2012) Fu-Cheng Liang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The bacterial Sec-translocase: structure and mechanism

(2012) J. A. Lycklama a Nijeholt et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Preserving the membrane barrier for small molecules during bacterial protein translocation

(2011) Eunyong Park et al.   NATURE

Lateral opening of a translocon upon entry of protein suggests the mechanism of insertion into membranes

(2010) P. F. Egea et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Charged Amino Acids in a Preprotein Inhibit SecA-Dependent Protein Translocation

(2009) Nico Nouwen et al.   JOURNAL OF MOLECULAR BIOLOGY

Conformational Flexibility and Peptide Interaction of the Translocation ATPase SecA

(2009) Jochen Zimmer et al.   JOURNAL OF MOLECULAR BIOLOGY

Modulation of protein properties in living cells using nanobodies

(2009) Axel Kirchhofer et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Mapping polypeptide interactions of the SecA ATPase during translocation

(2009) B. W. Bauer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A role for the two-helix finger of the SecA ATPase in protein translocation

(2008) Karl J. Erlandson et al.   NATURE

Structure of a complex of the ATPase SecA and the protein-translocation channel

(2008) Jochen Zimmer et al.   NATURE

Conformational transition of Sec machinery inferred from bacterial SecYE structures

(2008) Tomoya Tsukazaki et al.   NATURE