A High‐Throughput Automated Confocal Microscopy Platform for Quantitative Phenotyping of Nanoparticle Uptake and Transport in Spheroids

3D Cell-Based Assays for Drug Screens: Challenges in Imaging, Image Analysis, and High-Content Analysis

(2019) Tijmen H. Booij et al.   SLAS Discovery

Penetration and Uptake of Nanoparticles in 3D Tumor Spheroids

(2019) Aleksandra Tchoryk et al.   BIOCONJUGATE CHEMISTRY

Machine Learning Enables Live Label-Free Phenotypic Screening in Three Dimensions

(2018) Eoghan O'Duibhir et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

In vivo therapeutic applications of cell spheroids

(2018) Chin Siang Ong et al.   BIOTECHNOLOGY ADVANCES

High-content imaging assays on a miniaturized 3D cell culture platform

(2018) Pranav Joshi et al.   TOXICOLOGY IN VITRO

Novel 3D Liquid Cell Culture Method for Anchorage-independent Cell Growth, Cell Imaging and Automated Drug Screening

(2018) Natsuki Abe-Fukasawa et al.   Scientific Reports

A high-throughput imaging and nuclear segmentation analysis protocol for cleared 3D culture models

(2018) Molly E. Boutin et al.   Scientific Reports

4D cell biology: big data image analytics and lattice light-sheet imaging reveal dynamics of clathrin-mediated endocytosis in stem cell derived intestinal organoids

(2018) Johannes Schöneberg et al.   MOLECULAR BIOLOGY OF THE CELL

Progress and potential in organoid research

(2018) Giuliana Rossi et al.   NATURE REVIEWS GENETICS

Nanoparticles Penetrate into the Multicellular Spheroid-on-Chip: Effect of Surface Charge, Protein Corona, and Exterior Flow

(2017) Ke Huang et al.   MOLECULAR PHARMACEUTICS

Systematic investigation on the intracellular trafficking network of polymeric nanoparticles

(2017) Jinxie Zhang et al.   Nanoscale

Surface Engineering of Nanoparticles for Targeted Delivery to Hepatocellular Carcinoma

(2017) Jingxiong Lu et al.   Small

Multiscale cytometry and regulation of 3D cell cultures on a chip

(2017) Sébastien Sart et al.   Nature Communications

Intracellular trafficking and cellular uptake mechanism of PHBV nanoparticles for targeted delivery in epithelial cell lines

(2017) Juan P. Peñaloza et al.   JOURNAL OF NANOBIOTECHNOLOGY

High-Content Monitoring of Drug Effects in a 3D Spheroid Model

(2017) Frédérique Mittler et al.   Frontiers in Oncology

Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures

(2016) Paulina S. Mrozowska et al.   JOURNAL OF CELL BIOLOGY

Intracellular Trafficking Network of Protein Nanocapsules: Endocytosis, Exocytosis and Autophagy

(2016) Jinxie Zhang et al.   Theranostics

Three-dimensional microtissues essentially contribute to preclinical validations of therapeutic targets in breast cancer

(2016) Natalie Falkenberg et al.   Cancer Medicine

A systematic High-Content Screening microscopy approach reveals key roles for Rab33b, OATL1 and Myo6 in nanoparticle trafficking in HeLa cells

(2016) Angela Panarella et al.   Scientific Reports

High-Content Assays for Characterizing the Viability and Morphology of 3D Cancer Spheroid Cultures

(2015) Oksana Sirenko et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

High-content analysis for drug delivery and nanoparticle applications

(2015) David J. Brayden et al.   DRUG DISCOVERY TODAY

High-throughput fluorescence imaging approaches for drug discovery usingin vitroandin vivothree-dimensional models

(2015) Natalia J Martinez et al.   Expert Opinion on Drug Discovery

Effect of Shape, Size, and Aspect Ratio on Nanoparticle Penetration and Distribution inside Solid Tissues Using 3D Spheroid Models

(2015) Rachit Agarwal et al.   Advanced Healthcare Materials

Enhanced transcellular penetration and drug delivery by crosslinked polymeric micelles into pancreatic multicellular tumor spheroids

(2015) Hongxu Lu et al.   Biomaterials Science

Spherical Cancer Models in Tumor Biology

(2015) Louis-Bastien Weiswald et al.   NEOPLASIA

3D high-content screening for the identification of compounds that target cells in dormant tumor spheroid regions

(2014) Carsten Wenzel et al.   EXPERIMENTAL CELL RESEARCH

3D spheroid model of mIMCD3 cells for studying ciliopathies and renal epithelial disorders

(2014) Rachel H Giles et al.   Nature Protocols

Quantification of Dynamic Morphological Drug Responses in 3D Organotypic Cell Cultures by Automated Image Analysis

(2014) Ville Härmä et al.   PLoS One

High-Throughput Screening (HTS) of Anticancer Drug Efficacy on a Micropillar/Microwell Chip Platform

(2013) Dong Woo Lee et al.   ANALYTICAL CHEMISTRY

New views on cellular uptake and trafficking of manufactured nanoparticles

(2013) L. Treuel et al.   Journal of the Royal Society Interface

Development of drug loaded nanoparticles for tumor targeting. Part 2: Enhancement of tumor penetration through receptor mediated transcytosis in 3D tumor models

(2013) Mohammad H. El-Dakdouki et al.   Nanoscale

Time resolved study of cell death mechanisms induced by amine-modified polystyrene nanoparticles

(2013) Fengjuan Wang et al.   Nanoscale

High-Throughput 3D Screening Reveals Differences in Drug Sensitivities between Culture Models of JIMT1 Breast Cancer Cells

(2013) Vesa Hongisto et al.   PLoS One

Mammosphere Formation in Breast Carcinoma Cell Lines Depends upon Expression of E-cadherin

(2013) Juan Manuel Iglesias et al.   PLoS One

Silencing of Mammalian Sar1 Isoforms Reveals COPII-Independent Protein Sorting and Transport

(2013) Meritxell B. Cutrona et al.   TRAFFIC

Colloidal systems for drug delivery: from design to therapy

(2012) Mariana Beija et al.   TRENDS IN BIOTECHNOLOGY

Effect of shell-crosslinking of micelles on endocytosis and exocytosis: acceleration of exocytosis by crosslinking

(2012) Yoseop Kim et al.   Biomaterials Science

OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation

(2011) Takashi Itoh et al.   JOURNAL OF CELL BIOLOGY

Endocytosis and intracellular transport of nanoparticles: Present knowledge and need for future studies

(2011) Tore-Geir Iversen et al.   Nano Today

High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array

(2010) Yi-Chung Tung et al.   ANALYST

In situ protein expression in tumour spheres: development of an immunostaining protocol for confocal microscopy

(2010) Louis-Bastien Weiswald et al.   BMC CANCER

Synergistic Enhancement of Carboplatin Efficacy with Photodynamic Therapy in a Three-Dimensional Model for Micrometastatic Ovarian Cancer

(2010) I. Rizvi et al.   CANCER RESEARCH

Incorporation of multicellular spheroids into 3-D polymeric scaffolds provides an improved tumor model for screening anticancer drugs

(2010) Won Jin Ho et al.   CANCER SCIENCE

Rab7b controls trafficking from endosomes to the TGN

(2010) C. Progida et al.   JOURNAL OF CELL SCIENCE

Logic-Embedded Vectors for Intracellular Partitioning, Endosomal Escape, and Exocytosis of Nanoparticles

(2010) Rita E. Serda et al.   Small

Spheroid-based drug screen: considerations and practical approach

(2009) Juergen Friedrich et al.   Nature Protocols

Rab GTPases as coordinators of vesicle traffic

(2009) Harald Stenmark   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Golgi-resident Small GTPase Rab33B Interacts with Atg16L and Modulates Autophagosome Formation

(2008) Takashi Itoh et al.   MOLECULAR BIOLOGY OF THE CELL