Non-canonical ubiquitination of the cholesterol-regulated degron of squalene monooxygenase

A chemical biology screen identifies a vulnerability of neuroendocrine cancer cells to SQLE inhibition

(2019) Christopher E. Mahoney et al.   Nature Communications

Squalene accumulation in cholesterol auxotrophic lymphomas prevents oxidative cell death

(2019) Javier Garcia-Bermudez et al.   NATURE

Characterisation of the interaction between arginine methyltransferase Hmt1 and its substrate Npl3: use of multiple crosslinkers, mass spectrometry approaches and software platforms

(2018) Daniela-Lee Smith et al.   ANALYTICAL CHEMISTRY

Squalene epoxidase as a promising metabolic target in cancer treatment

(2018) Gabriella Cirmena et al.   CANCER LETTERS

MARCH6 and TRC8 facilitate the quality control of cytosolic and tail‐anchored proteins

(2018) Sandra Stefanovic‐Barrett et al.   EMBO REPORTS

Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity

(2018) Kuan-Chuan Pao et al.   NATURE

UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites

(2018) Vyacheslav Akimov et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Squalene epoxidase drives NAFLD-induced hepatocellular carcinoma and is a pharmaceutical target

(2018) Dabin Liu et al.   Science Translational Medicine

UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites

(2018) Vyacheslav Akimov et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Differential use of E2 ubiquitin conjugating enzymes for regulated degradation of the rate-limiting enzymes HMGCR and SQLE in cholesterol biosynthesis

(2018) Josephine M.E. Tan et al.   ATHEROSCLEROSIS

A cholesterol-sensing mechanism unfolds

(2017) William A. Prinz   JOURNAL OF BIOLOGICAL CHEMISTRY

A conserved degron containing an amphipathic helix regulates the cholesterol-mediated turnover of human squalene monooxygenase, a rate-limiting enzyme in cholesterol synthesis

(2017) Ngee Kiat Chua et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Phosphorylation regulates activity of 7-dehydrocholesterol reductase (DHCR7), a terminal enzyme of cholesterol synthesis

(2017) Anika V. Prabhu et al.   JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

Cholesterol Modification of Smoothened Is Required for Hedgehog Signaling

(2017) Xu Xiao et al.   MOLECULAR CELL

Cholesterol and fatty acids regulate cysteine ubiquitylation of ACAT2 through competitive oxidation

(2017) Yong-Jian Wang et al.   NATURE CELL BIOLOGY

Site-specific identification and quantitation of endogenous SUMO modifications under native conditions

(2017) Ryan J. Lumpkin et al.   Nature Communications

Cholesterol Metabolism and Prostate Cancer Lethality

(2016) K. H. Stopsack et al.   CANCER RESEARCH

The Role of Cholesterol in Cancer

(2016) Omer F. Kuzu et al.   CANCER RESEARCH

Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C

(2016) Nicholas G. Brown et al.   CELL

E2 enzymes: more than just middle men

(2016) Mikaela D Stewart et al.   CELL RESEARCH

Design Principles Involving Protein Disorder Facilitate Specific Substrate Selection and Degradation by the Ubiquitin-Proteasome System

(2016) Mainak Guharoy et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Sequential Poly-ubiquitylation by Specialized Conjugating Enzymes Expands the Versatility of a Quality Control Ubiquitin Ligase

(2016) Annika Weber et al.   MOLECULAR CELL

Multiomic Analysis of the UV-Induced DNA Damage Response

(2016) Stefan Boeing et al.   Cell Reports

Structural disorder and its role in proteasomal degradation

(2015) Antje Aufderheide et al.   FEBS LETTERS

The Regulatory Domain of Squalene Monooxygenase Contains a Re-entrant Loop and Senses Cholesterol via a Conformational Change

(2015) Vicky Howe et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Sequence composition of disordered regions fine-tunes protein half-life

(2015) Susan Fishbain et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Sequence composition of disordered regions fine-tunes protein half-life

(2015) Susan Fishbain et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Suberoylanilide Hydroxamic Acid Treatment Reveals Crosstalks among Proteome, Ubiquitylome and Acetylome in Non-Small Cell Lung Cancer A549 Cell Line

(2015) Quan Wu et al.   Scientific Reports

The human ubiquitin conjugating enzyme UBE2J2 (Ubc6) is a substrate for proteasomal degradation

(2014) Shuet Y. Lam et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

The sterol-based transcriptional control of human 7-dehydrocholesterol reductase (DHCR7): Evidence of a cooperative regulatory program in cholesterol synthesis

(2014) Anika V. Prabhu et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

DISOPRED3: precise disordered region predictions with annotated protein-binding activity

(2014) David T. Jones et al.   BIOINFORMATICS

Atypical Ubiquitylation in Yeast Targets Lysine-less Asi2 for Proteasomal Degradation

(2014) Mirta Boban et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Stoichiometry of Saccharomyces cerevisiae Lysine Methylation: Insights into Non-histone Protein Lysine Methyltransferase Activity

(2014) Gene Hart-Smith et al.   JOURNAL OF PROTEOME RESEARCH

The E3 Ubiquitin Ligase MARCH6 Degrades Squalene Monooxygenase and Affects 3-Hydroxy-3-Methyl-Glutaryl Coenzyme A Reductase and the Cholesterol Synthesis Pathway

(2014) N. Zelcer et al.   MOLECULAR AND CELLULAR BIOLOGY

Lysine-targeting specificity in ubiquitin and ubiquitin-like modification pathways

(2014) Francesca Mattiroli et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

PhosphoSitePlus, 2014: mutations, PTMs and recalibrations

(2014) Peter V. Hornbeck et al.   NUCLEIC ACIDS RESEARCH

USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation

(2014) Yanfen Liu et al.   eLife

Squalene epoxidase as a target for manipulation of squalene levels in the yeastSaccharomyces cerevisiae

(2013) Martina Garaiová et al.   FEMS YEAST RESEARCH

Non-canonical ubiquitylation: Mechanisms and consequences

(2013) Gary S. McDowell et al.   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

Signaling regulates activity of DHCR24, the final enzyme in cholesterol synthesis

(2013) Winnie Luu et al.   JOURNAL OF LIPID RESEARCH

Cholesterol as a causative factor in Alzheimer's disease: a debatable hypothesis

(2013) W. Gibson Wood et al.   JOURNAL OF NEUROCHEMISTRY

Metabolic engineering of Saccharomyces cerevisiae for production of ginsenosides

(2013) Zhubo Dai et al.   METABOLIC ENGINEERING

A suppressor screen in Mecp2 mutant mice implicates cholesterol metabolism in Rett syndrome

(2013) Christie M Buchovecky et al.   NATURE GENETICS

Integrated proteomic analysis of post-translational modifications by serial enrichment

(2013) Philipp Mertins et al.   NATURE METHODS

Proteome-wide mapping of cholesterol-interacting proteins in mammalian cells

(2013) Jonathan J Hulce et al.   NATURE METHODS

Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation

(2013) Danielle L Swaney et al.   NATURE METHODS

Large-scale identification of ubiquitination sites by mass spectrometry

(2013) Namrata D Udeshi et al.   Nature Protocols

Sterol homeostasis requires regulated degradation of squalene monooxygenase by the ubiquitin ligase Doa10/Teb4

(2013) Ombretta Foresti et al.   eLife

Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data

(2012) M. Kearse et al.   BIOINFORMATICS

Cholesterol through the Looking Glass

(2012) Ika Kristiana et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Squalene monooxygenase – a target for hypercholesterolemic therapy

(2011) Agnieszka Belter et al.   BIOLOGICAL CHEMISTRY

The Molecular Basis of CRL4DDB2/CSA Ubiquitin Ligase Architecture, Targeting, and Activation

(2011) Eric S. Fischer et al.   CELL

Cholesterol-Dependent Degradation of Squalene Monooxygenase, a Control Point in Cholesterol Synthesis beyond HMG-CoA Reductase

(2011) Saloni Gill et al.   Cell Metabolism

Cooperation between an Intrinsically Disordered Region and a Helical Segment Is Required for Ubiquitin-independent Degradation by the Proteasome

(2011) Sandra P. Melo et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Detection and Characterization of Low Abundance Glycopeptides Via Higher-Energy C-Trap Dissociation and Orbitrap Mass Analysis

(2011) Gene Hart-Smith et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Defining human ERAD networks through an integrative mapping strategy

(2011) John C. Christianson et al.   NATURE CELL BIOLOGY

Cysteine Ubiquitination of PTS1 Receptor Pex5p Regulates Pex5p Recycling

(2011) Kanji Okumoto et al.   TRAFFIC

Serine Residues in the Cytosolic Tail of the T-cell Antigen Receptor α-Chain Mediate Ubiquitination and Endoplasmic Reticulum-associated Degradation of the Unassembled Protein

(2010) Shuhei Ishikura et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Malformation syndromes caused by disorders of cholesterol synthesis

(2010) Forbes D. Porter et al.   JOURNAL OF LIPID RESEARCH

Ubiquitylation of an ERAD Substrate Occurs on Multiple Types of Amino Acids

(2010) Yuichiro Shimizu et al.   MOLECULAR CELL

Ube2j2 ubiquitinates hydroxylated amino acids on ER-associated degradation substrates

(2009) Xiaoli Wang et al.   JOURNAL OF CELL BIOLOGY

Building ubiquitin chains: E2 enzymes at work

(2009) Yihong Ye et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

HELIQUEST: a web server to screen sequences with specific  -helical properties

(2008) R. Gautier et al.   BIOINFORMATICS

Feedback regulation of cholesterol synthesis: sterol-accelerated ubiquitination and degradation of HMG CoA reductase

(2008) Russell A DeBose-Boyd   CELL RESEARCH

Iodoacetamide-induced artifact mimics ubiquitination in mass spectrometry

(2008) Michael L Nielsen et al.   NATURE METHODS

Diversity of degradation signals in the ubiquitin–proteasome system

(2008) Tommer Ravid et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY