Transformation techniques for metabolic engineering of diatoms and haptophytes: current state and prospects

CRISPR technologies for bacterial systems: Current achievements and future directions

(2016) Kyeong Rok Choi et al.   BIOTECHNOLOGY ADVANCES

Nuclear transformation of the diatom Phaeodactylum tricornutum using PCR-amplified DNA fragments by microparticle bombardment

(2016) Nozomu Kira et al.   Marine Genomics

Silencing UDP-glucose pyrophosphorylase gene in Phaeodactylum tricornutum affects carbon allocation

(2016) Bao-Hua Zhu et al.   New Biotechnology

A CRISPR/Cas9 system adapted for gene editing in marine algae

(2016) Marianne Nymark et al.   Scientific Reports

Use of a lipid rich strain reveals mechanisms of nitrogen limitation and carbon partitioning in the haptophyte Tisochrysis lutea

(2016) Matthieu Garnier et al.   Algal Research-Biomass Biofuels and Bioproducts

Genetic Manipulation of Competition for Nitrate between Heterotrophic Bacteria and Diatoms

(2016) Rachel E. Diner et al.   Frontiers in Microbiology

Transgene Expression in Microalgae—From Tools to Applications

(2016) Lior Doron et al.   Frontiers in Plant Science

Rapid induction of GFP expression by the nitrate reductase promoter in the diatomPhaeodactylum tricornutum

(2016) Lili Chu et al.   PeerJ

Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae

(2016) Hirotoshi Endo et al.   Scientific Reports

Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity

(2015) Masaki Muto et al.   Biotechnology for Biofuels

Chloroplast-targeting protein expression in the oleaginous diatom Fistulifera solaris JPCC DA0580 toward metabolic engineering

(2015) Yoshihiko Sunaga et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Establishment of Genetic Transformation in the Sexually Reproducing Diatoms Pseudo-nitzschia multistriata and Pseudo-nitzschia arenysensis and Inheritance of the Transgene

(2015) Valeria Sabatino et al.   MARINE BIOTECHNOLOGY

Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum

(2015) Takashi Kadono et al.   Marine Drugs

Designer diatom episomes delivered by bacterial conjugation

(2015) Bogumil J. Karas et al.   Nature Communications

Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum

(2015) Takashi Kadono et al.   Scientific Reports

Development of a new constitutive expression system for the transformation of the diatom Phaeodactylum tricornutum

(2015) Seungbeom Seo et al.   Algal Research-Biomass Biofuels and Bioproducts

Agrobacterium tumefaciens-mediated genetic transformation of haptophytes (Isochrysis species)

(2014) Binod Prasad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

(2014) Wenzhi Jiang et al.   EUKARYOTIC CELL

Construction of Novel Chloroplast Expression Vector and Development of an Efficient Transformation System for the Diatom Phaeodactylum tricornutum

(2014) Wei-Hong Xie et al.   MARINE BIOTECHNOLOGY

Localization of enzymes relating to C4 organic acid metabolisms in the marine diatom, Thalassiosira pseudonana

(2014) Rie Tanaka et al.   PHOTOSYNTHESIS RESEARCH

A stable and efficient nuclear transformation system for the diatom Chaetoceros gracilis

(2014) Kentaro Ifuku et al.   PHOTOSYNTHESIS RESEARCH

Localization of putative carbonic anhydrases in the marine diatom, Thalassiosira pseudonana

(2014) Mio Samukawa et al.   PHOTOSYNTHESIS RESEARCH

Inactivation of Phaeodactylum tricornutum urease gene using transcription activator-like effector nuclease-based targeted mutagenesis

(2014) Philip D. Weyman et al.   PLANT BIOTECHNOLOGY JOURNAL

A Tyrosine-Rich Cell Surface Protein in the Diatom Amphora coffeaeformis Identified through Transcriptome Analysis and Genetic Transformation

(2014) Matthias T. Buhmann et al.   PLoS One

Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology

(2014) Fayza Daboussi et al.   Nature Communications

The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): Illuminating the Functional Diversity of Eukaryotic Life in the Oceans through Transcriptome Sequencing

(2014) Patrick J. Keeling et al.   PLOS BIOLOGY

Highly Efficient Transformation of the DiatomPhaeodactylum tricornutumby Multi-Pulse Electroporation

(2013) Mado MIYAHARA et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Translocation of cell penetrating peptides onChlamydomonas reinhardtii

(2013) Arumuganainar Suresh et al.   BIOTECHNOLOGY AND BIOENGINEERING

Improvement of Neutral Lipid and Polyunsaturated Fatty Acid Biosynthesis by Overexpressing a Type 2 Diacylglycerol Acyltransferase in Marine Diatom Phaeodactylum tricornutum

(2013) Ying-Fang Niu et al.   Marine Drugs

High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation

(2013) Chunye Zhang et al.   Marine Genomics

Metabolic engineering of Phaeodactylum tricornutum for the enhanced accumulation of omega-3 long chain polyunsaturated fatty acids

(2013) Mary L. Hamilton et al.   METABOLIC ENGINEERING

Pan genome of the phytoplankton Emiliania underpins its global distribution

(2013) Betsy A. Read et al.   NATURE

Molecular and Photosynthetic Responses to Prolonged Darkness and Subsequent Acclimation to Re-Illumination in the Diatom Phaeodactylum tricornutum

(2013) Marianne Nymark et al.   PLoS One

Aureochrome 1a Is Involved in the Photoacclimation of the Diatom Phaeodactylum tricornutum

(2013) Benjamin Schellenberger Costa et al.   PLoS One

Metabolic engineering of lipid catabolism increases microalgal lipid accumulation without compromising growth

(2013) E. M. Trentacoste et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Transformation of diatom Phaeodactylum tricornutum by electroporation and establishment of inducible selection marker

(2012) Ying-Fang Niu et al.   BIOTECHNIQUES

A molecular method for the delivery of small molecules and proteins across the cell wall of algae using molecular transporters

(2012) J. M. Hyman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production of unique immunotoxin cancer therapeutics in algal chloroplasts

(2012) M. Tran et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome and low-iron response of an oceanic diatom adapted to chronic iron limitation

(2012) Markus Lommer et al.   GENOME BIOLOGY

LC-PUFA from photosynthetic microalgae: occurrence, biosynthesis, and prospects in biotechnology

(2011) Inna Khozin-Goldberg et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Compensatory response of the unicellular-calcifying alga Emiliania huxleyi (Coccolithophoridales, Haptophyta) to ocean acidification

(2011) Shin-ya Fukuda et al.   JOURNAL OF OCEANOGRAPHY

Agrobacterium tumefaciens mediated transformation of marine microalgae Schizochytrium

(2011) Rubin Cheng et al.   MICROBIOLOGICAL RESEARCH

Localization of putative carbonic anhydrases in two marine diatoms, Phaeodactylum tricornutum and Thalassiosira pseudonana

(2011) Masaaki Tachibana et al.   PHOTOSYNTHESIS RESEARCH

Characterization of the Selenite Uptake Mechanism in the Coccolithophore Emiliania huxleyi (Haptophyta)

(2011) H. Araie et al.   PLANT AND CELL PHYSIOLOGY

Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives

(2011) Sergio Rosales-Mendoza et al.   PLANT CELL REPORTS

Effect of Coccolith Polysaccharides Isolated from the Coccolithophorid, Emiliania huxleyi, on Calcite Crystal Formation in In Vitro CaCO3 Crystallization

(2010) Keisuke Kayano et al.   MARINE BIOTECHNOLOGY

Genetic engineering of fatty acid chain length in Phaeodactylum tricornutum

(2010) Randor Radakovits et al.   METABOLIC ENGINEERING

FIRST INDUCED PLASTID GENOME MUTATIONS IN AN ALGA WITH SECONDARY PLASTIDS: psbA MUTATIONS IN THE DIATOM PHAEODACTYLUM TRICORNUTUM (BACILLARIOPHYCEAE) REVEAL CONSEQUENCES ON THE REGULATION OF PHOTOSYNTHESIS1

(2009) Arne C. Materna et al.   JOURNAL OF PHYCOLOGY

ERAD-Derived Preprotein Transport across the Second Outermost Plastid Membrane of Diatoms

(2009) F. Hempel et al.   MOLECULAR BIOLOGY AND EVOLUTION

Gene silencing in the marine diatom Phaeodactylum tricornutum

(2009) Valentina De Riso et al.   NUCLEIC ACIDS RESEARCH

The Phaeodactylum genome reveals the evolutionary history of diatom genomes

(2008) Chris Bowler et al.   NATURE

Development of gene expression system in a marine diatom using viral promoters of a wide variety of origin

(2008) Kunihiro Sakaue et al.   PHYSIOLOGIA PLANTARUM