Physical decoupling of XylS/Pm regulatory elements and conditional proteolysis enable precise control of gene expression in Pseudomonas putida

Genetic tools for reliable gene expression and recombineering in Pseudomonas putida

(2018) Taylor B. Cook et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Pseudomonas putida as a functional chassis for industrial biocatalysis: From native biochemistry to trans -metabolism

(2018) Pablo I. Nikel et al.   METABOLIC ENGINEERING

Engineered promoters enable constant gene expression at any copy number in bacteria

(2018) Thomas H Segall-Shapiro et al.   NATURE BIOTECHNOLOGY

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non-traditional microorganisms

(2018) Patricia Calero et al.   Microbial Biotechnology

A Post-translational Metabolic Switch Enables Complete Decoupling of Bacterial Growth from Biopolymer Production in Engineered Escherichia coli

(2018) Gonzalo Durante-Rodríguez et al.   ACS Synthetic Biology

Deconvolution of Gene Expression Noise into Spatial Dynamics of Transcription Factor–Promoter Interplay

(2017) Ángel Goñi-Moreno et al.   ACS Synthetic Biology

The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering

(2017) Agnieszka Gawin et al.   Microbial Biotechnology

From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactions

(2016) Pablo I Nikel et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes

(2016) Ilaria Benedetti et al.   METABOLIC ENGINEERING

Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR

(2016) Michael Jahn et al.   Microbial Cell Factories

Metabolic Burden: Cornerstones in Synthetic Biology and Metabolic Engineering Applications

(2016) Gang Wu et al.   TRENDS IN BIOTECHNOLOGY

Broad-Host-Range ProUSER Vectors Enable Fast Characterization of Inducible Promoters and Optimization of p-Coumaric Acid Production in Pseudomonas putida KT2440

(2016) Patricia Calero et al.   ACS Synthetic Biology

A Metabolic Widget Adjusts the Phosphoenolpyruvate-Dependent Fructose Influx in Pseudomonas putida

(2016) Max Chavarría et al.   mSystems

Pseudomonas putidaKT2440 Strain Metabolizes Glucose through a Cycle Formed by Enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and Pentose Phosphate Pathways

(2015) Pablo I. Nikel et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Glycerol-Dependent Metabolic Persistence of Pseudomonas putida KT2440 Reflects the Regulatory Logic of the GlpR Repressor

(2015) Pablo I. Nikel et al.   mBio

Accurate DNA Assembly and Genome Engineering with Optimized Uracil Excision Cloning

(2015) Ana Mafalda Cavaleiro et al.   ACS Synthetic Biology

Tn7-Based Device for Calibrated Heterologous Gene Expression in Pseudomonas putida

(2015) Sebastian Zobel et al.   ACS Synthetic Biology

SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities

(2014) Esteban Martínez-García et al.   NUCLEIC ACIDS RESEARCH

Regulation of the expression level of transcription factor XylS reveals new functional insight into its induction mechanism at the Pm promoter

(2013) Friederike Zwick et al.   BMC MICROBIOLOGY

Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette

(2012) Friederike Zwick et al.   Microbial Cell Factories

Foundations for the design and implementation of synthetic genetic circuits

(2012) Adrian L. Slusarczyk et al.   NATURE REVIEWS GENETICS

The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes

(2012) Rafael Silva-Rocha et al.   NUCLEIC ACIDS RESEARCH

The logic layout of the TOL network of Pseudomonas putida pWW0 plasmid stems from a metabolic amplifier motif (MAM) that optimizes biodegradation of m-xylene

(2011) Rafael Silva-Rocha et al.   BMC Systems Biology

Random Mutagenesis of the Pm Promoter as a Powerful Strategy for Improvement of Recombinant-Gene Expression

(2009) I. Bakke et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Directed evolution of the transcription factor XylS for development of improved expression systems

(2009) Trond Erik Vee Aune et al.   Microbial Biotechnology