Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR

DNA copy number concentration measured by digital and droplet digital quantitative PCR using certified reference materials

(2015) Philippe Corbisier et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Polar Fixation of Plasmids during Recombinant Protein Production in Bacillus megaterium Results in Population Heterogeneity

(2015) Karin M. Münch et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Non-random distribution of macromolecules as driving forces for phenotypic variation

(2015) Michael Jahn et al.   CURRENT OPINION IN MICROBIOLOGY

Variability in subpopulation formation propagates into biocatalytic variability of engineered Pseudomonas putida strains

(2015) Martin Lindmeyer et al.   Frontiers in Microbiology

Standards not that standard

(2015) Cristina Vilanova et al.   Journal of Biological Engineering

Accurate Determination of Plasmid Copy Number of Flow-Sorted Cells using Droplet Digital PCR

(2014) Michael Jahn et al.   ANALYTICAL CHEMISTRY

A reduced genome decreases the host carrying capacity for foreign DNA

(2014) Yuya Akeno et al.   Microbial Cell Factories

SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities

(2014) Esteban Martínez-García et al.   NUCLEIC ACIDS RESEARCH

The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

(2013) J. F. Huggett et al.   CLINICAL CHEMISTRY

A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli

(2013) Simone Balzer et al.   Microbial Cell Factories

Evaluation of three industrial Escherichia coli strains in fed-batch cultivations during high-level SOD protein production

(2013) Karoline Marisch et al.   Microbial Cell Factories

Cytometric fingerprinting for analyzing microbial intracommunity structure variation and identifying subcommunity function

(2013) Christin Koch et al.   Nature Protocols

Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

(2012) Jian Ye et al.   BMC BIOINFORMATICS

Subpopulation-proteomics in prokaryotic populations

(2012) Michael Jahn et al.   CURRENT OPINION IN BIOTECHNOLOGY

The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes

(2012) Rafael Silva-Rocha et al.   NUCLEIC ACIDS RESEARCH

Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR

(2011) Kang Zhou et al.   BMC MOLECULAR BIOLOGY

Growth-Rate Dependence Reveals Design Principles of Plasmid Copy Number Control

(2011) Stefan Klumpp   PLoS One

Rapid optimization of gene dosage in E. coli using DIAL strains

(2011) Joshua T Kittleson et al.   Journal of Biological Engineering

Bistability, Epigenetics, and Bet-Hedging in Bacteria

(2008) Jan-Willem Veening et al.   Annual Review of Microbiology

Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes

(2008) Mihaela Skulj et al.   Microbial Cell Factories

Engineering BioBrick vectors from BioBrick parts

(2008) Reshma P Shetty et al.   Journal of Biological Engineering