The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering

Metabolic engineering of Pseudomonas putida for production of docosahexaenoic acid based on a myxobacterial PUFA synthase

(2016) Katja Gemperlein et al.   METABOLIC ENGINEERING

A large family of anti-activators accompanying XylS/AraC family regulatory proteins

(2016) Araceli E. Santiago et al.   MOLECULAR MICROBIOLOGY

Comparative Single-Cell Analysis of Different E. coli Expression Systems during Microfluidic Cultivation

(2016) Dennis Binder et al.   PLoS One

Alginate Biosynthesis Factories in Pseudomonas fluorescens: Localization and Correlation with Alginate Production Level

(2015) Susan Maleki et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A three-step method for analysing bacterial biofilm formation under continuous medium flow

(2015) Karolin Schmutzler et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

How do bacteria tune translation efficiency?

(2015) Gene-Wei Li   CURRENT OPINION IN MICROBIOLOGY

Benzoic Acid-Inducible Gene Expression in Mycobacteria

(2015) Marte S. Dragset et al.   PLoS One

Metabolic Engineering of Pseudomonas putida KT2440 to Produce Anthranilate from Glucose

(2015) Jannis Kuepper et al.   Frontiers in Microbiology

Engineering low-temperature expression systems for heterologous production of cold-adapted enzymes

(2015) Gro Elin Kjæreng Bjerga et al.   Bioengineered

Engineering of Pseudomonas taiwanensis VLB120 for Constitutive Solvent Tolerance and Increased Specific Styrene Epoxidation Activity

(2014) Jan Volmer et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Vibrio cholerae ghosts (VCG) exert immunomodulatory effect on dendritic cells for enhanced antigen presentation and induction of protective immunity

(2014) Francis O Eko et al.   BMC IMMUNOLOGY

Genetically modified microorganism Spingomonas paucimobilis UT26 for simultaneously degradation of methyl-parathion and γ-hexachlorocyclohexane

(2014) Wen S. Lan et al.   ECOTOXICOLOGY

The pWW0 plasmid imposes a stochastic expression regime to the chromosomalorthopathway for benzoate metabolism inPseudomonas putida

(2014) Rafael Silva-Rocha et al.   FEMS MICROBIOLOGY LETTERS

Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression

(2014) Esteban Martínez-García et al.   Microbial Cell Factories

Roles of genomic island 3 (GI-3) BAB1_0267 and BAB1_0270 open reading frames (ORFs) in the virulence of Brucella abortus 2308

(2014) Luisa Ortiz-Román et al.   VETERINARY MICROBIOLOGY

Recombinant protein expression in Escherichia coli: advances and challenges

(2014) Germán L. Rosano et al.   Frontiers in Microbiology

Improved cytotoxic effects ofSalmonella-producing cytosine deaminase in tumour cells

(2014) Beatriz Mesa-Pereira et al.   Microbial Biotechnology

Design and Optimization of Short DNA Sequences That Can Be Used as 5′ Fusion Partners for High-Level Expression of Heterologous Genes in Escherichia coli

(2013) Veronika Kucharova et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

High production of recombinant Norwegian salmonid alphavirus E1 and E2 proteins in Escherichia coli by fusion to secretion signal sequences and removal of hydrophobic domains

(2013) Anne Tøndervik et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

Regulation of the expression level of transcription factor XylS reveals new functional insight into its induction mechanism at the Pm promoter

(2013) Friederike Zwick et al.   BMC MICROBIOLOGY

A second chromosomal copy of thecatAgene endowsPseudomonas putida mt-2 with an enzymatic safety valve for excess of catechol

(2013) Jose I. Jiménez et al.   ENVIRONMENTAL MICROBIOLOGY

A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli

(2013) Simone Balzer et al.   Microbial Cell Factories

Development of a broad-host synthetic biology toolbox for ralstonia eutropha and its application to engineering hydrocarbon biofuel production

(2013) Changhao Bi et al.   Microbial Cell Factories

Identification of Genes Involved in Pseudomonas aeruginosa Biofilm-Specific Resistance to Antibiotics

(2013) Li Zhang et al.   PLoS One

Novel Tools to Analyze the Function of Salmonella Effectors Show That SvpB Ectopic Expression Induces Cell Cycle Arrest in Tumor Cells

(2013) Beatriz Mesa-Pereira et al.   PLoS One

Combinatorial Mutagenesis and Selection of Improved Signal Sequences and Their Application for High-Level Production of Translocated Heterologous Proteins in Escherichia coli

(2012) Tonje Marita Bjerkan Heggeset et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The Biofilm-Specific Antibiotic Resistance Gene ndvB Is Important for Expression of Ethanol Oxidation Genes in Pseudomonas aeruginosa Biofilms

(2012) T. Beaudoin et al.   JOURNAL OF BACTERIOLOGY

Fe protein over-expression can enhance the nitrogenase activity ofAzotobacter vinelandii

(2012) Papri Nag et al.   JOURNAL OF BASIC MICROBIOLOGY

Engineering an anaerobic metabolic regime in Pseudomonas putida KT2440 for the anoxic biodegradation of 1,3-dichloroprop-1-ene

(2012) Pablo I. Nikel et al.   METABOLIC ENGINEERING

Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette

(2012) Friederike Zwick et al.   Microbial Cell Factories

The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes

(2012) Rafael Silva-Rocha et al.   NUCLEIC ACIDS RESEARCH

Continuous Control of the Flow in Biochemical Pathways through 5′ Untranslated Region Sequence Modifications in mRNA Expressed from the Broad-Host-Range PromoterPm

(2011) Rahmi Lale et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The logic layout of the TOL network of Pseudomonas putida pWW0 plasmid stems from a metabolic amplifier motif (MAM) that optimizes biodegradation of m-xylene

(2011) Rafael Silva-Rocha et al.   BMC Systems Biology

Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440

(2011) Esteban Martínez-García et al.   ENVIRONMENTAL MICROBIOLOGY

Pseudomonas aeruginosa tssC1 Links Type VI Secretion and Biofilm-Specific Antibiotic Resistance

(2011) L. Zhang et al.   JOURNAL OF BACTERIOLOGY

Exploring the 5′-UTR DNA region as a target for optimizing recombinant gene expression from the strong and inducible Pm promoter in Escherichia coli

(2011) Laila Berg et al.   JOURNAL OF BIOTECHNOLOGY

Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory

(2011) Thorben Dammeyer et al.   Microbial Cell Factories

Improved Expression Systems for Regulated Expression in Salmonella Infecting Eukaryotic Cells

(2011) Carlos Medina et al.   PLoS One

Controlled autolysis facilitates the polyhydroxyalkanoate recovery in Pseudomonas putida KT2440

(2011) Virginia Martínez et al.   Microbial Biotechnology

Characterization of the Protocatechuate 4,5-Cleavage Pathway Operon in Comamonas sp. Strain E6 and Discovery of a Novel Pathway Gene

(2010) N. Kamimura et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enhancing the protein production levels in Escherichia coli with a strong promoter

(2010) Hanna Tegel et al.   FEBS Journal

Biosynthetic Pathway for  -Cyclic Sarcinaxanthin in Micrococcus luteus: Heterologous Expression and Evidence for Diverse and Multiple Catalytic Functions of C50 Carotenoid Cyclases

(2010) R. Netzer et al.   JOURNAL OF BACTERIOLOGY

Sequential XylS-CTD Binding to the Pm Promoter Induces DNA Bending Prior to Activation

(2010) P. Dominguez-Cuevas et al.   JOURNAL OF BACTERIOLOGY

Random Mutagenesis of the Pm Promoter as a Powerful Strategy for Improvement of Recombinant-Gene Expression

(2009) I. Bakke et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Characterization of the Isophthalate Degradation Genes of Comamonas sp. Strain E6

(2009) Y. Fukuhara et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Cloning, heterologous expression, and functional characterization of the nicotinate dehydrogenase gene from Pseudomonas putida KT2440

(2009) Yao Yang et al.   BIODEGRADATION

EcI5, a group IIB intron with high retrohoming frequency: DNA target site recognition and use in gene targeting

(2009) F. Zhuang et al.   RNA

The expression of recombinant genes inEscherichia colican be strongly stimulated at the transcript production level by mutating the DNA-region corresponding to the 5′-untranslated part of mRNA

(2009) Laila Berg et al.   Microbial Biotechnology

Directed evolution of the transcription factor XylS for development of improved expression systems

(2009) Trond Erik Vee Aune et al.   Microbial Biotechnology

The Azotobacter vinelandii AlgE mannuronan C-5-epimerase family is essential for the in vivo control of alginate monomer composition and for functional cyst formation

(2008) Magnus Steigedal et al.   ENVIRONMENTAL MICROBIOLOGY

Involvement of a Novel Efflux System in Biofilm-Specific Resistance to Antibiotics

(2008) L. Zhang et al.   JOURNAL OF BACTERIOLOGY

Positively regulated bacterial expression systems

(2008) Trygve Brautaset et al.   Microbial Biotechnology

XylS–Pm Promoter Interactions through Two Helix–Turn–Helix Motifs: Identifying XylS Residues Important for DNA Binding and Activation

(2007) Patricia Domínguez-Cuevas et al.   JOURNAL OF MOLECULAR BIOLOGY