Genetic tools for reliable gene expression and recombineering in Pseudomonas putida

CRISPR/Cas9-Based Counterselection Boosts Recombineering Efficiency in Pseudomonas putida

(2017) Tomás Aparicio et al.   Biotechnology Journal

A metabolic pathway for catabolizing levulinic acid in bacteria

(2017) Jacqueline M. Rand et al.   Nature Microbiology

The Ssr protein (T1E_1405) fromPseudomonas putidaDOT-T1E enables oligonucleotide-based recombineering in platform strainP. putidaEM42

(2016) Tomás Aparicio et al.   Biotechnology Journal

From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactions

(2016) Pablo I Nikel et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Broad-Host-Range ProUSER Vectors Enable Fast Characterization of Inducible Promoters and Optimization of p-Coumaric Acid Production in Pseudomonas putida KT2440

(2016) Patricia Calero et al.   ACS Synthetic Biology

Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli

(2016) Marcelo C. Bassalo et al.   ACS Synthetic Biology

The Ssr protein (T1E_1405) fromPseudomonas putidaDOT-T1E enables oligonucleotide-based recombineering in platform strainP. putidaEM42

(2016) Tomás Aparicio et al.   Biotechnology Journal

Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli

(2015) Michael E. Pyne et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Pseudomonas putida—a versatile host for the production of natural products

(2015) Anita Loeschcke et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Tn7-Based Device for Calibrated Heterologous Gene Expression in Pseudomonas putida

(2015) Sebastian Zobel et al.   ACS Synthetic Biology

Functional Characterization of the Mannitol Promoter of Pseudomonas fluorescens DSM 50106 and Its Application for a Mannitol-Inducible Expression System for Pseudomonas putida KT2440

(2015) Jana Hoffmann et al.   PLoS One

CasOT: a genome-wide Cas9/gRNA off-target searching tool

(2014) A. Xiao et al.   BIOINFORMATICS

Biotechnological domestication of pseudomonads using synthetic biology

(2014) Pablo I. Nikel et al.   NATURE REVIEWS MICROBIOLOGY

CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri

(2014) Jee-Hwan Oh et al.   NUCLEIC ACIDS RESEARCH

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

(2014) Ryan E. Cobb et al.   ACS Synthetic Biology

Functional characterization and application of a tightly regulated MekR/P mekA expression system in Escherichia coli and Pseudomonas putida

(2013) Nadja Graf et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Genetic engineering of Pseudomonas putida KT2440 for rapid and high-yield production of vanillin from ferulic acid

(2013) Nadja Graf et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Broad host range plasmids

(2013) Aayushi Jain et al.   FEMS MICROBIOLOGY LETTERS

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Cas9 as a versatile tool for engineering biology

(2013) Prashant Mali et al.   NATURE METHODS

Heterologous Expression and Genetic Engineering of the Tubulysin Biosynthetic Gene Cluster Using Red/ET Recombineering and Inactivation Mutagenesis

(2012) Yi Chai et al.   CHEMISTRY & BIOLOGY

The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes

(2012) Rafael Silva-Rocha et al.   NUCLEIC ACIDS RESEARCH

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Development of a Method for Markerless Gene Deletion in Pseudomonas putida

(2011) Nadja Graf et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440

(2011) Esteban Martínez-García et al.   ENVIRONMENTAL MICROBIOLOGY

Nonlinear electrophoresis for purification of soil DNA for metagenomics

(2011) Katja Engel et al.   JOURNAL OF MICROBIOLOGICAL METHODS

BglBrick vectors and datasheets: A synthetic biology platform for gene expression

(2011) Taek Lee et al.   Journal of Biological Engineering

Recombineering Using RecTE from Pseudomonas syringae

(2010) B. Swingle et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Broad-host-range vectors for protein expression across gram negative hosts

(2010) Jamie E. Prior et al.   BIOTECHNOLOGY AND BIOENGINEERING

Recent advances in awakening silent biosynthetic gene clusters and linking orphan clusters to natural products in microorganisms

(2010) Yi-Ming Chiang et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

The Escherichia coli rhamnose promoter rhaP BAD is in Pseudomonas putida KT2440 independent of Crp–cAMP activation

(2009) Marcel Jeske et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering Pseudomonas putida S12 for Efficient Utilization of D-Xylose and L-Arabinose

(2008) J.-P. Meijnen et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Use of the lambda Red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa

(2008) Biliana Lesic et al.   BMC MOLECULAR BIOLOGY