Accelerated genome engineering of Pseudomonas putida by I-Sce I―mediated recombination and CRISPR-Cas9 counterselection

Protocols for RecET-based markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida

(2019) Kyeong Rok Choi et al.   Microbial Biotechnology

Genetic tools for reliable gene expression and recombineering in Pseudomonas putida

(2018) Taylor B. Cook et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida

(2018) Kyeong Rok Choi et al.   METABOLIC ENGINEERING

Pseudomonas putida as a functional chassis for industrial biocatalysis: From native biochemistry to trans -metabolism

(2018) Pablo I. Nikel et al.   METABOLIC ENGINEERING

Metabolic engineering of Pseudomonas taiwanensis VLB120 with minimal genomic modifications for high-yield phenol production

(2018) Benedikt Wynands et al.   METABOLIC ENGINEERING

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non-traditional microorganisms

(2018) Patricia Calero et al.   Microbial Biotechnology

CRISPR/Cas9-Based Counterselection Boosts Recombineering Efficiency in Pseudomonas putida

(2017) Tomás Aparicio et al.   Biotechnology Journal

The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering

(2017) Agnieszka Gawin et al.   Microbial Biotechnology

From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactions

(2016) Pablo I Nikel et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

The revisited genome of Pseudomonas putida KT2440 enlightens its value as a robust metabolic chassis

(2016) Eugeni Belda et al.   ENVIRONMENTAL MICROBIOLOGY

Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes

(2016) Ilaria Benedetti et al.   METABOLIC ENGINEERING

Broad-Host-Range ProUSER Vectors Enable Fast Characterization of Inducible Promoters and Optimization of p-Coumaric Acid Production in Pseudomonas putida KT2440

(2016) Patricia Calero et al.   ACS Synthetic Biology

Pseudomonas putida—a versatile host for the production of natural products

(2015) Anita Loeschcke et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Pseudomonas putidaKT2440 Strain Metabolizes Glucose through a Cycle Formed by Enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and Pentose Phosphate Pathways

(2015) Pablo I. Nikel et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Phenotypic knockouts of selected metabolic pathways by targeting enzymes with camel-derived nanobodies (V HH s)

(2015) José I. Jiménez et al.   METABOLIC ENGINEERING

Accurate DNA Assembly and Genome Engineering with Optimized Uracil Excision Cloning

(2015) Ana Mafalda Cavaleiro et al.   ACS Synthetic Biology

Quantifying Impact of Chromosome Copy Number on Recombination in Escherichia coli

(2015) T. Steele Reynolds et al.   ACS Synthetic Biology

Tn7-Based Device for Calibrated Heterologous Gene Expression in Pseudomonas putida

(2015) Sebastian Zobel et al.   ACS Synthetic Biology

The Functional Structure of Central Carbon Metabolism in Pseudomonas putida KT2440

(2014) Suresh Sudarsan et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Biotechnological domestication of pseudomonads using synthetic biology

(2014) Pablo I. Nikel et al.   NATURE REVIEWS MICROBIOLOGY

SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities

(2014) Esteban Martínez-García et al.   NUCLEIC ACIDS RESEARCH

Software-Supported USER Cloning Strategies for Site-Directed Mutagenesis and DNA Assembly

(2014) Hans Jasper Genee et al.   ACS Synthetic Biology

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Precise and reliable gene expression via standard transcription and translation initiation elements

(2013) Vivek K Mutalik et al.   NATURE METHODS

The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes

(2012) Rafael Silva-Rocha et al.   NUCLEIC ACIDS RESEARCH

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%

(2012) Joachim Goedhart et al.   Nature Communications

CRISPR-Cas Systems in Bacteria and Archaea: Versatile Small RNAs for Adaptive Defense and Regulation

(2011) Devaki Bhaya et al.   Annual Review of Genetics

Development of a Method for Markerless Gene Deletion in Pseudomonas putida

(2011) Nadja Graf et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440

(2011) Esteban Martínez-García et al.   ENVIRONMENTAL MICROBIOLOGY

An Enhanced Monomeric Blue Fluorescent Protein with the High Chemical Stability of the Chromophore

(2011) Oksana M. Subach et al.   PLoS One

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Improving the photostability of bright monomeric orange and red fluorescent proteins

(2008) Nathan C Shaner et al.   NATURE METHODS

Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440

(2008) J. I. Jimenez et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA