Precise and reliable gene expression via standard transcription and translation initiation elements

Contextualizing context for synthetic biology - identifying causes of failure of synthetic biological systems

(2012) Stefano Cardinale et al.   Biotechnology Journal

Synthetic RNA switches as a tool for temporal and spatial control over gene expression

(2012) Andrew L Chang et al.   CURRENT OPINION IN BIOTECHNOLOGY

Successes and failures in modular genetic engineering

(2012) Joshua T Kittleson et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Directed evolution: an evolving and enabling synthetic biology tool

(2012) Ryan E Cobb et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

A translation-coupling DNA cassette for monitoring protein translation in Escherichia coli

(2012) Daniel Mendez-Perez et al.   METABOLIC ENGINEERING

RNA processing enables predictable programming of gene expression

(2012) Lei Qi et al.   NATURE BIOTECHNOLOGY

Ribozyme-based insulator parts buffer synthetic circuits from genetic context

(2012) Chunbo Lou et al.   NATURE BIOTECHNOLOGY

Rationally designed families of orthogonal RNA regulators of translation

(2012) Vivek K Mutalik et al.   Nature Chemical Biology

Rewritable digital data storage in live cells via engineered control of recombination directionality

(2012) J. Bonnet et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca

(2012) K. Temme et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Toward rational design of bacterial genomes

(2011) Guillaume Cambray et al.   CURRENT OPINION IN MICROBIOLOGY

DNA assembly for synthetic biology: from parts to pathways and beyond

(2011) Tom Ellis et al.   Integrative Biology

Mechanism of Bacterial Transcription Initiation: RNA Polymerase - Promoter Binding, Isomerization to Initiation-Competent Open Complexes, and Initiation of RNA Synthesis

(2011) Ruth M. Saecker et al.   JOURNAL OF MOLECULAR BIOLOGY

Targeted proteomics for metabolic pathway optimization: Application to terpene production

(2011) Alyssa M. Redding-Johanson et al.   METABOLIC ENGINEERING

The ribosome uses two active mechanisms to unwind messenger RNA during translation

(2011) Xiaohui Qu et al.   NATURE

Regulation of transcription by unnatural amino acids

(2011) Chang C Liu et al.   NATURE BIOTECHNOLOGY

Predicting the strength of UP-elements and full-length E. coli σE promoters

(2011) Virgil A. Rhodius et al.   NUCLEIC ACIDS RESEARCH

BglBrick vectors and datasheets: A synthetic biology platform for gene expression

(2011) Taek Lee et al.   Journal of Biological Engineering

Single Ribosome Dynamics and the Mechanism of Translation

(2010) Colin Echeverría Aitken et al.   Annual Review of Biophysics

Five hard truths for synthetic biology

(2010) Roberta Kwok   NATURE

Design, construction and characterization of a set of insulated bacterial promoters

(2010) J. H. Davis et al.   NUCLEIC ACIDS RESEARCH

Predicting strength and function for promoters of the Escherichia coli alternative sigma factor,  E

(2010) V. A. Rhodius et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Manufacturing Molecules Through Metabolic Engineering

(2010) J. D. Keasling   SCIENCE

Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

(2010) D. G. Gibson et al.   SCIENCE

The Fitness Landscapes of cis-Acting Binding Sites in Different Promoter and Environmental Contexts

(2010) Ryan K. Shultzaberger et al.   PLoS Genetics

Promoter Strength Properties of the Complete Sigma E Regulon of Escherichia coli and Salmonella enterica

(2009) V. K. Mutalik et al.   JOURNAL OF BACTERIOLOGY

You're one in a googol: optimizing genes for protein expression

(2009) M. Welch et al.   Journal of the Royal Society Interface

A new method for the construction of translationally coupled operons in a bacterial chromosome

(2009) A. Yu. Gulevich et al.   MOLECULAR BIOLOGY

Genome engineering

(2009) Peter A Carr et al.   NATURE BIOTECHNOLOGY

Building outside of the box: iGEM and the BioBricks Foundation

(2009) Christina D Smolke   NATURE BIOTECHNOLOGY

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

Next-generation synthetic gene networks

(2009) Timothy K Lu et al.   NATURE BIOTECHNOLOGY

The second wave of synthetic biology: from modules to systems

(2009) Priscilla E. M. Purnick et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Mechanism of sequence-specific pausing of bacterial RNA polymerase

(2009) M. L. Kireeva et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Coding-Sequence Determinants of Gene Expression in Escherichia coli

(2009) G. Kudla et al.   SCIENCE

A xyloglucan-specific family 12 glycosyl hydrolase fromAspergillus niger: recombinant expression, purification and characterization

(2008) Emma R. Master et al.   BIOCHEMICAL JOURNAL

Refinement and standardization of synthetic biological parts and devices

(2008) Barry Canton et al.   NATURE BIOTECHNOLOGY

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One