Protocols for RecET-based markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida

Genetic tools for reliable gene expression and recombineering in Pseudomonas putida

(2018) Taylor B. Cook et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida

(2018) Kyeong Rok Choi et al.   METABOLIC ENGINEERING

Pseudomonas putida as a functional chassis for industrial biocatalysis: From native biochemistry to trans -metabolism

(2018) Pablo I. Nikel et al.   METABOLIC ENGINEERING

Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system

(2018) Jun Sun et al.   Microbial Cell Factories

CRISPR/Cas9-coupled recombineering for metabolic engineering of Corynebacterium glutamicum

(2017) Jae Sung Cho et al.   METABOLIC ENGINEERING

Dissemination of antibiotic resistance genes from antibiotic producers to pathogens

(2017) Xinglin Jiang et al.   Nature Communications

CRISPR/Cas9-Based Counterselection Boosts Recombineering Efficiency in Pseudomonas putida

(2017) Tomás Aparicio et al.   Biotechnology Journal

The Ssr protein (T1E_1405) fromPseudomonas putidaDOT-T1E enables oligonucleotide-based recombineering in platform strainP. putidaEM42

(2016) Tomás Aparicio et al.   Biotechnology Journal

From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactions

(2016) Pablo I Nikel et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

The Ssr protein (T1E_1405) fromPseudomonas putidaDOT-T1E enables oligonucleotide-based recombineering in platform strainP. putidaEM42

(2016) Tomás Aparicio et al.   Biotechnology Journal

EngineeringPseudomonas putidaKT2440 for simultaneous degradation of carbofuran and chlorpyrifos

(2016) Ting Gong et al.   Microbial Biotechnology

Pseudomonas putida—a versatile host for the production of natural products

(2015) Anita Loeschcke et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

ThreePseudomonas putidaFNR Family Proteins with Different Sensitivities to O2

(2015) Susan A. Ibrahim et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Seven gene deletions in seven days: Fast generation of Escherichia coli strains tolerant to acetate and osmotic stress

(2015) Sheila I. Jensen et al.   Scientific Reports

Efficient recombinant production of prodigiosin in Pseudomonas putida

(2015) Andreas Domröse et al.   Frontiers in Microbiology

Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression

(2014) Esteban Martínez-García et al.   Microbial Cell Factories

Rapid one-step inactivation of single or multiple genes inEscherichia coli

(2013) Chan Woo Song et al.   Biotechnology Journal

Heterologous Expression and Genetic Engineering of the Tubulysin Biosynthetic Gene Cluster Using Red/ET Recombineering and Inactivation Mutagenesis

(2012) Yi Chai et al.   CHEMISTRY & BIOLOGY

Construction of a stable genetically engineered rhamnolipid-producing microorganism for remediation of pyrene-contaminated soil

(2012) Li Cao et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

TREX: A Universal Tool for the Transfer and Expression of Biosynthetic Pathways in Bacteria

(2012) Anita Loeschcke et al.   ACS Synthetic Biology

Development of a Method for Markerless Gene Deletion in Pseudomonas putida

(2011) Nadja Graf et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440

(2011) Esteban Martínez-García et al.   ENVIRONMENTAL MICROBIOLOGY

Site-specific chromosomal integration of large synthetic constructs

(2010) Thomas E. Kuhlman et al.   NUCLEIC ACIDS RESEARCH

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS