Dynamic structural biology at the protein membrane interface

UCT943, a next generation Plasmodium falciparum PI4K inhibitor preclinical candidate for the treatment of malaria

(2018) Christel Brunschwig et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

The invasin D protein fromYersinia pseudotuberculosisselectively binds the Fab region of host antibodies and affects colonization of the intestine

(2018) Pooja Sadana et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Novel K-Ras G12C Switch-II Covalent Binders Destabilize Ras and Accelerate Nucleotide Exchange

(2018) Chimno I. Nnadi et al.   Journal of Chemical Information and Modeling

An intrinsic lipid-binding interface controls sphingosine kinase 1 function

(2018) Michael J. Pulkoski-Gross et al.   JOURNAL OF LIPID RESEARCH

Conformational sampling of membranes by Akt controls its activation and inactivation

(2018) Iva Lučić et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecular Mechanisms of Human Disease Mediated by Oncogenic and Primary Immunodeficiency Mutations in Class IA Phosphoinositide 3-Kinases

(2018) Gillian L. Dornan et al.   Frontiers in Immunology

Structural Basis for Regulation of Phosphoinositide Kinases and Their Involvement in Human Disease

(2018) John E. Burke   MOLECULAR CELL

Structural determinants of Rab11 activation by the guanine nucleotide exchange factor SH3BP5

(2018) Meredith L. Jenkins et al.   Nature Communications

Neolymphostin A Is a Covalent Phosphoinositide 3-Kinase (PI3K)/Mammalian Target of Rapamycin (mTOR) Dual Inhibitor That Employs an Unusual Electrophilic Vinylogous Ester

(2018) Gabriel Castro-Falcón et al.   JOURNAL OF MEDICINAL CHEMISTRY

Structural and mechanistic insights into the function of the unconventional class XIV myosin MyoA fromToxoplasma gondii

(2018) Cameron J. Powell et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An overview of hydrogen deuterium exchange mass spectrometry (HDX-MS) in drug discovery

(2017) Glenn R. Masson et al.   Expert Opinion on Drug Discovery

Novel PIK3CD mutations affecting N-terminal residues of p110δ cause activated PI3Kδ syndrome (APDS) in humans

(2017) Andrew J. Takeda et al.   JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

Dissecting the molecular assembly of theToxoplasma gondiiMyoA motility complex

(2017) Cameron J. Powell et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Molecular mechanism of activation of class IA phosphoinositide 3-kinases (PI3Ks) by membrane-localized HRas

(2017) Braden D. Siempelkamp et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

PI(3,4,5)P 3 Engagement Restricts Akt Activity to Cellular Membranes

(2017) Michael Ebner et al.   MOLECULAR CELL

A Cryptosporidium PI(4)K inhibitor is a drug candidate for cryptosporidiosis

(2017) Ujjini H. Manjunatha et al.   NATURE

Conformational disruption of PI3Kδ regulation by immunodeficiency mutations inPIK3CDandPIK3R1

(2017) Gillian L. Dornan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Antimalarial efficacy of MMV390048, an inhibitor of Plasmodium phosphatidylinositol 4-kinase

(2017) Tanya Paquet et al.   Science Translational Medicine

Kobuviral Non-structural 3A Proteins Act as Molecular Harnesses to Hijack the Host ACBD3 Protein

(2017) Martin Klima et al.   STRUCTURE

The Molecular Basis of Aichi Virus 3A Protein Activation of Phosphatidylinositol 4 Kinase IIIβ, PI4KB, through ACBD3

(2017) Jacob A. McPhail et al.   STRUCTURE

Characterization of Atg38 and NRBF2, a fifth subunit of the autophagic Vps34/PIK3C3 complex

(2016) Yohei Ohashi et al.   Autophagy

Type III phosphatidylinositol 4 kinases: structure, function, regulation, signalling and involvement in disease

(2016) G. L. Dornan et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Design and Structural Characterization of Potent and Selective Inhibitors of Phosphatidylinositol 4 Kinase IIIβ

(2016) Florentine U. Rutaganira et al.   JOURNAL OF MEDICINAL CHEMISTRY

Diversity-oriented synthesis yields novel multistage antimalarial inhibitors

(2016) Nobutaka Kato et al.   NATURE

Using hydrogen deuterium exchange mass spectrometry to engineer optimized constructs for crystallization of protein complexes: Case study of PI4KIIIβ with Rab11

(2016) Melissa L. Fowler et al.   PROTEIN SCIENCE

Fat(al) attraction: Picornaviruses Usurp Lipid Transfer at Membrane Contact Sites to Create Replication Organelles

(2016) Hilde M. van der Schaar et al.   TRENDS IN MICROBIOLOGY

Structural insights and in vitro reconstitution of membrane targeting and activation of human PI4KB by the ACBD3 protein

(2016) Martin Klima et al.   Scientific Reports

Analytical Aspects of Hydrogen Exchange Mass Spectrometry

(2015) John R. Engen et al.   Annual Review of Analytical Chemistry

The function of phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) explored using a specific inhibitor that targets the PI5P-binding site

(2015) Jonathan H. Clarke et al.   BIOCHEMICAL JOURNAL

The intrinsically disordered tails of PTEN and PTEN-L have distinct roles in regulating substrate specificity and membrane activity

(2015) G. R. Masson et al.   BIOCHEMICAL JOURNAL

Probing the dynamic regulation of peripheral membrane proteins using hydrogen deuterium exchange-MS (HDX-MS)

(2015) O. Vadas et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Structure of Rab11–FIP3–Rabin8 reveals simultaneous binding of FIP3 and Rabin8 effectors to Rab11

(2015) Melanie Vetter et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure and flexibility of the endosomal Vps34 complex reveals the basis of its function on membranes

(2015) K. Rostislavleva et al.   SCIENCE

Synergy in activating class I PI3Ks

(2015) John E. Burke et al.   TRENDS IN BIOCHEMICAL SCIENCES

Structure of Rab11–FIP3–Rabin8 reveals simultaneous binding of FIP3 and Rabin8 effectors to Rab11

(2015) Melanie Vetter et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A Complex Comprising Phosphatidylinositol 4-Kinase III , ACBD3, and Aichi Virus Proteins Enhances Phosphatidylinositol 4-Phosphate Synthesis and Is Critical for Formation of the Viral Replication Complex

(2014) K. Ishikawa-Sasaki et al.   JOURNAL OF VIROLOGY

Structures of PI4KIII  complexes show simultaneous recruitment of Rab11 and its effectors

(2014) J. E. Burke et al.   SCIENCE

Mutations in PIK3R1 Cause SHORT Syndrome

(2013) David A. Dyment et al.   AMERICAN JOURNAL OF HUMAN GENETICS

Vesicular and non-vesicular transport feed distinct glycosylation pathways in the Golgi

(2013) Giovanni D’Angelo et al.   NATURE

Targeting Plasmodium PI(4)K to eliminate malaria

(2013) Case W. McNamara et al.   NATURE

Dominant-activating germline mutations in the gene encoding the PI(3)K catalytic subunit p110δ result in T cell senescence and human immunodeficiency

(2013) Carrie L Lucas et al.   NATURE IMMUNOLOGY

Phosphoinositides: Tiny Lipids With Giant Impact on Cell Regulation

(2013) Tamas Balla   PHYSIOLOGICAL REVIEWS

Molecular determinants of PI3K -mediated activation downstream of G-protein-coupled receptors (GPCRs)

(2013) O. Vadas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Phosphoinositide 3-Kinase   Gene Mutation Predisposes to Respiratory Infection and Airway Damage

(2013) I. Angulo et al.   SCIENCE

PKCβ Phosphorylates PI3Kγ to Activate It and Release It from GPCR Control

(2013) Romy Walser et al.   PLOS BIOLOGY

Using Hydrogen/Deuterium Exchange Mass Spectrometry to Define the Specific Interactions of the Phospholipase A2Superfamily with Lipid Substrates, Inhibitors, and Membranes

(2012) Jian Cao et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The 3A Protein from Multiple Picornaviruses Utilizes the Golgi Adaptor Protein ACBD3 To Recruit PI4KIII 

(2012) A. L. Greninger et al.   JOURNAL OF VIROLOGY

Mosaic overgrowth with fibroadipose hyperplasia is caused by somatic activating mutations in PIK3CA

(2012) Marjorie J Lindhurst et al.   NATURE GENETICS

De novo germline and postzygotic mutations in AKT3, PIK3R2 and PIK3CA cause a spectrum of related megalencephaly syndromes

(2012) Jean-Baptiste Rivière et al.   NATURE GENETICS

Oncogenic mutations mimic and enhance dynamic events in the natural activation of phosphoinositide 3-kinase p110  (PIK3CA)

(2012) J. E. Burke et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

G Protein-Coupled Receptor-Mediated Activation of p110  by G   Is Required for Cellular Transformation and Invasiveness

(2012) H. A. Dbouk et al.   Science Signaling

Phosphatidylinositol 4-kinases: hostages harnessed to build panviral replication platforms

(2012) Nihal Altan-Bonnet et al.   TRENDS IN BIOCHEMICAL SCIENCES

ACBD3-mediated recruitment of PI4KB to picornavirus RNA replication sites

(2011) Jun Sasaki et al.   EMBO JOURNAL

Structural Basis for Activation and Inhibition of Class I Phosphoinositide 3-Kinases

(2011) Oscar Vadas et al.   Science Signaling

Dynamics of the Phosphoinositide 3-Kinase p110δ Interaction with p85α and Membranes Reveals Aspects of Regulation Distinct from p110α

(2011) John E. Burke et al.   STRUCTURE

Hydrogen exchange mass spectrometry for studying protein structure and dynamics

(2010) Lars Konermann et al.   CHEMICAL SOCIETY REVIEWS

Localizing the Membrane Binding Region of Group VIA Ca2+-independent Phospholipase A2Using Peptide Amide Hydrogen/Deuterium Exchange Mass Spectrometry

(2009) Yuan-Hao Hsu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Dual roles for theDrosophilaPI 4-kinase Four wheel drive in localizing Rab11 during cytokinesis

(2009) Gordon Polevoy et al.   JOURNAL OF CELL BIOLOGY

Location of Inhibitors Bound to Group IVA Phospholipase A2Determined by Molecular Dynamics and Deuterium Exchange Mass Spectrometry

(2009) John E. Burke et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Crystallizing membrane proteins using lipidic mesophases

(2009) Martin Caffrey et al.   Nature Protocols

Construct optimization for protein NMR structure analysis using amide hydrogen/deuterium exchange mass spectrometry

(2009) Seema Sharma et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Interaction of Group IA Phospholipase A2with Metal Ions and Phospholipid Vesicles Probed with Deuterium Exchange Mass Spectrometry†

(2008) John E. Burke et al.   BIOCHEMISTRY

Group IVA cytosolic phospholipase A2 (cPLA2 ) and integrin  IIb 3 reinforce each other's functions during  IIb 3 signaling in platelets

(2008) N. Prevost et al.   BLOOD

Phospholipase A2 Biochemistry

(2008) John E. Burke et al.   CARDIOVASCULAR DRUGS AND THERAPY

A Phospholipid Substrate Molecule Residing in the Membrane Surface Mediates Opening of the Lid Region in Group IVA Cytosolic Phospholipase A2

(2008) John E. Burke et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Calcium Binding Rigidifies the C2 Domain and the Intradomain Interaction of GIVA Phospholipase A2as Revealed by Hydrogen/Deuterium Exchange Mass Spectrometry

(2008) Yuan-Hao Hsu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Phospholipase A2structure/function, mechanism, and signaling

(2008) John E. Burke et al.   JOURNAL OF LIPID RESEARCH

Lipid signalling in disease

(2008) Matthias P. Wymann et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY