Biochemical analysis with the expanded genetic lexicon

A Decade of Bioorthogonal Chemistry

(2011) Carolyn R. Bertozzi   ACCOUNTS OF CHEMICAL RESEARCH

Development of a Selective, Sensitive, and Reversible Biosensor by the Genetic Incorporation of a Metal-Binding Site into Green Fluorescent Protein

(2011) Niraikulam Ayyadurai et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Genetically Encoded Copper-Free Click Chemistry

(2011) Tilman Plass et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Fluorescent Reporter of the Phosphorylation Status of the Substrate Protein STAT3

(2011) Vanessa K. Lacey et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Photo-Cross-Linkers Incorporated into G-Protein-Coupled Receptors in Mammalian Cells: A Ligand Comparison

(2011) Irene Coin et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Using Fluorine Nuclear Magnetic Resonance To Probe Changes in the Structure and Dynamics of Membrane-Active Peptides Interacting with Lipid Bilayers

(2011) Yuta Suzuki et al.   BIOCHEMISTRY

Bioconjugation ofl-3,4-Dihydroxyphenylalanine Containing Protein with a Polysaccharide

(2011) Niraikulam Ayyadurai et al.   BIOCONJUGATE CHEMISTRY

Probing Protein-Protein Interactions with a Genetically Encoded Photo-crosslinking Amino Acid

(2011) Hui-wang Ai et al.   CHEMBIOCHEM

Subcellular Protein Localization by Using a Genetically Encoded Fluorescent Amino Acid

(2011) Godefroid Charbon et al.   CHEMBIOCHEM

A readily synthesized cyclic pyrrolysine analogue for site-specific protein “click” labeling

(2011) Ziyang Hao et al.   CHEMICAL COMMUNICATIONS

Palladium-mediated site-selective Suzuki–Miyaura protein modification at genetically encoded aryl halides

(2011) Christopher D. Spicer et al.   CHEMICAL COMMUNICATIONS

Probing Local Environments with the Infrared Probe:l-4-Nitrophenylalanine

(2011) Emily E. Smith et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Traceless and Site-Specific Ubiquitination of Recombinant Proteins

(2011) Satpal Virdee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Copper-Free Sonogashira Cross-Coupling for Functionalization of Alkyne-Encoded Proteins in Aqueous Medium and in Bacterial Cells

(2011) Nan Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Incorporation of Fluorotyrosines into Ribonucleotide Reductase Using an Evolved, Polyspecific Aminoacyl-tRNA Synthetase

(2011) Ellen C. Minnihan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Rapid Chemoselective Bioconjugation through Oxidative Coupling of Anilines and Aminophenols

(2011) Christopher R. Behrens et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Kinetics of Radical Intermediate Formation and Deoxynucleotide Production in 3-Aminotyrosine-SubstitutedEscherichia coliRibonucleotide Reductases

(2011) Ellen C. Minnihan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Expanding the Genetic Code of an Animal

(2011) Sebastian Greiss et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetically Encoded 1,2-Aminothiols Facilitate Rapid and Site-Specific Protein Labeling via a Bio-orthogonal Cyanobenzothiazole Condensation

(2011) Duy P. Nguyen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-Specific Fluorescent Labeling of Nascent Proteins on the Translating Ribosome

(2011) Ishu Saraogi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites

(2011) David B F Johnson et al.   Nature Chemical Biology

A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance

(2011) Meng Zhang et al.   Nature Chemical Biology

Bringing chemistry to life

(2011) Michael Boyce et al.   NATURE METHODS

Application of the logic of cysteine-free native chemical ligation to the synthesis of Human Parathyroid Hormone (hPTH)

(2011) S. Shang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genetically Encoding Unnatural Amino Acids in Neural Stem Cells and Optically Reporting Voltage-Sensitive Domain Changes in Differentiated Neurons

(2011) Bin Shen et al.   STEM CELLS

A Metabolic Alkene Reporter for Spatiotemporally Controlled Imaging of Newly Synthesized Proteins in Mammalian Cells

(2010) Wenjiao Song et al.   ACS Chemical Biology

A Facile System for Genetic Incorporation of Two Different Noncanonical Amino Acids into One Protein inEscherichia coli

(2010) Wei Wan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Site-Specific Incorporation of Fluorotyrosines into Proteins inEscherichia coliby Photochemical Disguise

(2010) Bryan J. Wilkins et al.   BIOCHEMISTRY

Labeling Live Cells by Copper-Catalyzed Alkyne−Azide Click Chemistry

(2010) Vu Hong et al.   BIOCONJUGATE CHEMISTRY

Retrotranslocation of a Misfolded Luminal ER Protein by the Ubiquitin-Ligase Hrd1p

(2010) Pedro Carvalho et al.   CELL

A method to site-specifically introduce methyllysine into proteins in E. coli

(2010) Hui-wang Ai et al.   CHEMICAL COMMUNICATIONS

Residue-specific incorporation of non-canonical amino acids into proteins: recent developments and applications

(2010) Jeremiah A Johnson et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Beyond the Canonical 20 Amino Acids: Expanding the Genetic Lexicon

(2010) Travis S. Young et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Identification of Highly Reactive Sequences For PLP-Mediated Bioconjugation Using a Combinatorial Peptide Library

(2010) Leah S. Witus et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Biosynthetic Route to Photoclick Chemistry on Proteins

(2010) Jiangyun Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair

(2010) Susan M. Hancock et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

(2010) Ying Huang et al.   Molecular BioSystems

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

(2010) Heinz Neumann et al.   NATURE

Tracking G-protein-coupled receptor activation using genetically encoded infrared probes

(2010) Shixin Ye et al.   NATURE

Genetic Incorporation of Unnatural Amino Acids into Proteins in Mycobacterium tuberculosis

(2010) Feng Wang et al.   PLoS One

Expansion of the genetic code: Site-directed p-fluoro-phenylalanine incorporation in Escherichia coli

(2010) Rolf Furter   PROTEIN SCIENCE

A Pyrrolysine Analogue for Site-Specific Protein Ubiquitination

(2009) Xin Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Pyrrolysine Analogue for Protein Click Chemistry

(2009) Tomasz Fekner et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Genetically Encoded Alkenes in Yeast

(2009) Hui-wang Ai et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Facile System for Encoding Unnatural Amino Acids in Mammalian Cells

(2009) Peng R. Chen et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Interpretation ofp-Cyanophenylalanine Fluorescence in Proteins in Terms of Solvent Exposure and Contribution of Side-Chain Quenchers: A Combined Fluorescence, IR and Molecular Dynamics Study

(2009) Humeyra Taskent-Sezgin et al.   BIOCHEMISTRY

Use of 3-Aminotyrosine To Examine the Pathway Dependence of Radical Propagation inEscherichia coliRibonucleotide Reductase

(2009) Ellen C. Minnihan et al.   BIOCHEMISTRY

Click Chemistry and Bioorthogonal Reactions: Unprecedented Selectivity in the Labeling of Biological Molecules

(2009) Michael D. Best   BIOCHEMISTRY

Expanding the Genetic Repertoire of the Methylotrophic YeastPichia pastoris†

(2009) Travis S. Young et al.   BIOCHEMISTRY

Genetic incorporation of an aliphatic keto-containing amino acid into proteins for their site-specific modifications

(2009) Ying Huang et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Expanding the Genetic Code for Biological Studies

(2009) Qian Wang et al.   CHEMISTRY & BIOLOGY

Site-specific labeling of proteins with NMR-active unnatural amino acids

(2009) David H. Jones et al.   JOURNAL OF BIOMOLECULAR NMR

An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli

(2009) Travis S. Young et al.   JOURNAL OF MOLECULAR BIOLOGY

Genetic Incorporation of a Metal-Ion Chelating Amino Acid into Proteins as a Biophysical Probe

(2009) Hyun Soo Lee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Evolution of Proteins with Genetically Encoded “Chemical Warheads”

(2009) Chang C. Liu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetic Incorporation of a Small, Environmentally Sensitive, Fluorescent Probe into Proteins inSaccharomyces cerevisiae

(2009) Hyun Soo Lee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetic Encoding and Labeling of Aliphatic Azides and Alkynes in Recombinant Proteinsviaa Pyrrolysyl-tRNA Synthetase/tRNACUAPair and Click Chemistry

(2009) Duy P. Nguyen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

FTIR analysis of GPCR activation using azido probes

(2009) Shixin Ye et al.   Nature Chemical Biology

Site-directed spin labeling of a genetically encoded unnatural amino acid

(2009) M. R. Fleissner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Site-specific chemical modification of recombinant proteins produced in mammalian cells by using the genetically encoded aldehyde tag

(2009) P. Wu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genetic Encoding of 3-Iodo-l-Tyrosine in Escherichia coli for Single-Wavelength Anomalous Dispersion Phasing in Protein Crystallography

(2009) Kensaku Sakamoto et al.   STRUCTURE

A Genetically Encoded Boronate-Containing Amino Acid

(2008) Eric Brustad et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

An Engineered Aryl Azide Ligase for Site-Specific Mapping of Protein-Protein Interactions through Photo-Cross-Linking

(2008) Hemanta Baruah et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases

(2008) Takahito Mukai et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Binding is not enough: Flexibility is needed for photocrosslinking of Lck kinase by benzophenone photoligands

(2008) Akira Kawamura et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode Nɛ-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification

(2008) Tatsuo Yanagisawa et al.   CHEMISTRY & BIOLOGY

Genetically Encoding Protein Oxidative Damage

(2008) Heinz Neumann et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Selective Functionalization of a Genetically Encoded Alkene-Containing Protein via “Photoclick Chemistry” in Bacterial Cells

(2008) Wenjiao Song et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

In vivoIncorporation of Unnatural Amino Acids to Probe Structure, Dynamics, and Ligand Binding in a Large Protein by Nuclear Magnetic Resonance Spectroscopy

(2008) Susan E. Cellitti et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A General and Efficient Method for the Site-Specific Dual-Labeling of Proteins for Single Molecule Fluorescence Resonance Energy Transfer

(2008) Eric M. Brustad et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Cross-dressing proteins by olefin metathesis

(2008) Kent Kirshenbaum et al.   Nature Chemical Biology

Protein evolution with an expanded genetic code

(2008) C. C. Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA