Protein Organic Chemistry and Applications for Labeling and Engineering in Live-Cell Systems

Traceless Affinity Labeling of Endogenous Proteins for Functional Analysis in Living Cells

(2012) Takahiro Hayashi et al.   ACCOUNTS OF CHEMICAL RESEARCH

Catalytic Protein Modification with Dirhodium Metallopeptides: Specificity in Designed and Natural Systems

(2012) Zhen Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ligand-Directed Acyl Imidazole Chemistry for Labeling of Membrane-Bound Proteins on Live Cells

(2012) Sho-hei Fujishima et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Native FKBP12 Engineering by Ligand-Directed Tosyl Chemistry: Labeling Properties and Application to Photo-Cross-Linking of Protein Complexes in Vitro and in Living Cells

(2012) Tomonori Tamura et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-Specific Protein Modification with a Dirhodium Metallopeptide Catalyst

(2011) Zhen Chen et al.   ACS Chemical Biology

Phosphopeptide-Dependent Labeling of 14-3-3 ζ Proteins by Fusicoccin-Based Fluorescent Probes

(2011) Michiko Takahashi et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Construction of a 19F-lectin biosensor for glycoprotein imaging by using affinity-guided DMAP chemistry

(2011) Yedi Sun et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

A BODIPY-Cyclooctyne for Protein Imaging in Live Cells

(2011) Kimberly E. Beatty et al.   CHEMBIOCHEM

Proteome profiling reveals potential cellular targets of staurosporine using a clickable cell-permeable probe

(2011) Haibin Shi et al.   CHEMICAL COMMUNICATIONS

Design of a multinuclear Zn(ii) complex as a new molecular probe for fluorescence imaging of His-tag fused proteins

(2011) Sho-hei Fujishima et al.   CHEMICAL COMMUNICATIONS

Aliphatic Diazirines as Photoaffinity Probes for Proteins: Recent Developments

(2011) Joydip Das   CHEMICAL REVIEWS

A Bioorthogonal Quadricyclane Ligation

(2011) Ellen M. Sletten et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Potent and Selective Inhibitors of GlutathioneS-Transferase Omega 1 That Impair Cancer Drug Resistance

(2011) Katsunori Tsuboi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Small Molecule That Binds to an ATPase Domain of Hsc70 Promotes Membrane Trafficking of Mutant Cystic Fibrosis Transmembrane Conductance Regulator

(2011) Hyungseoph J. Cho et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemical Cell-Surface Receptor Engineering Using Affinity-Guided, Multivalent Organocatalysts

(2011) Hangxiang Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Live Cell Labeling of Native Intracellular Bacterial Receptors Using Aniline-Catalyzed Oxime Ligation

(2011) Josep Rayo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Design and Synthesis of Highly Reactive Dienophiles for the Tetrazine–trans-Cyclooctene Ligation

(2011) Michael T. Taylor et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Click-generated triazole ureas as ultrapotent in vivo–active serine hydrolase inhibitors

(2011) Alexander Adibekian et al.   Nature Chemical Biology

Small-molecule hydrophobic tagging–induced degradation of HaloTag fusion proteins

(2011) Taavi K Neklesa et al.   Nature Chemical Biology

Choosing an effective protein bioconjugation strategy

(2011) Nicholas Stephanopoulos et al.   Nature Chemical Biology

Target-selective fluorescent “switch-on” protein labeling by 6π-azaelectrocyclization

(2011) Katsunori Tanaka et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Site-specific protein modifications through pyrroline-carboxy-lysine residues

(2011) W. Ou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Site-Specific Orthogonal Labeling of the Carboxy Terminus of α-Tubulin

(2010) Abhijit Banerjee et al.   ACS Chemical Biology

Rhodamines NN: A Novel Class of Caged Fluorescent Dyes

(2010) Vladimir N. Belov et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Selective Covalent Labeling of Tag-Fused GPCR Proteins on Live Cell Surface with a Synthetic Probe for Their Functional Analysis

(2010) Hiroshi Nonaka et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Olefin Cross-Metathesis on Proteins: Investigation of Allylic Chalcogen Effects and Guiding Principles in Metathesis Partner Selection

(2010) Yuya A. Lin et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A fluorophore ligase for site-specific protein labeling inside living cells

(2010) C. Uttamapinant et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Copper-free click chemistry in living animals

(2010) P. V. Chang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mechanisms of chemical protein19F-labeling and NMR-based biosensor construction in vitro and in cells using self-assembling ligand-directed tosylate compounds

(2010) Yousuke Takaoka et al.   Chemical Science

Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality

(2009) Ellen M. Sletten et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

An In Vivo Fluorescent Sensor Reveals Intracellular Ins(1,3,4,5)P4Dynamics in Single Cells

(2009) Reiko Sakaguchi et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Recent Progress in Strategies for the Creation of Protein-Based Fluorescent Biosensors

(2009) Hangxiang Wang et al.   CHEMBIOCHEM

Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)–NTA probe

(2009) Sho-hei Uchinomiya et al.   CHEMICAL COMMUNICATIONS

Convenient modular method for affinity labeling (MoAL method) based on a catalytic amidation

(2009) Munetaka Kunishima et al.   CHEMICAL COMMUNICATIONS

Red-Emitting Rhodamine Dyes for Fluorescence Microscopy and Nanoscopy

(2009) Kirill Kolmakov et al.   CHEMISTRY-A EUROPEAN JOURNAL

Quenched Ligand-Directed Tosylate Reagents for One-Step Construction of Turn-On Fluorescent Biosensors

(2009) Shinya Tsukiji et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Marinopyrrole A Target Elucidation by Acyl Dye Transfer

(2009) Chambers C. Hughes et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Development of a Fluorescent-Tagged Kinase Assay System for the Detection and Characterization of Allosteric Kinase Inhibitors

(2009) Jeffrey R. Simard et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Convenient Catalyst for Aqueous and Protein Suzuki−Miyaura Cross-Coupling

(2009) Justin M. Chalker et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemoselective Tryptophan Labeling with Rhodium Carbenoids at Mild pH

(2009) John M. Antos et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Selective Labeling of Living Cells by a Photo-Triggered Click Reaction

(2009) Andrei A. Poloukhtine et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Optogenetic dissection of a behavioural module in the vertebrate spinal cord

(2009) Claire Wyart et al.   NATURE

A new screening assay for allosteric inhibitors of cSrc

(2009) Jeffrey R Simard et al.   Nature Chemical Biology

Ligand-directed tosyl chemistry for protein labeling in vivo

(2009) Shinya Tsukiji et al.   Nature Chemical Biology

High-efficiency labeling of sialylated glycoproteins on living cells

(2009) Ying Zeng et al.   NATURE METHODS

HaloTag7: A genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification

(2009) Rachel Friedman Ohana et al.   PROTEIN EXPRESSION AND PURIFICATION

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

N-terminal labeling of proteins by the Pictet–Spengler reaction

(2008) Tsubasa Sasaki et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Site-Specific Protein Modification on Living Cells Catalyzed by Sortase

(2008) Tsutomu Tanaka et al.   CHEMBIOCHEM

An Engineered Protein Tag for Multiprotein Labeling in Living Cells

(2008) Arnaud Gautier et al.   CHEMISTRY & BIOLOGY

Affinity‐Labeling‐Based Introduction of a Reactive Handle for Natural Protein Modification

(2008) Haruto Wakabayashi et al.   Chemistry-An Asian Journal

Selective Functionalization of a Genetically Encoded Alkene-Containing Protein via “Photoclick Chemistry” in Bacterial Cells

(2008) Wenjiao Song et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Facile Conversion of Cysteine and Alkyl Cysteines to Dehydroalanine on Protein Surfaces: Versatile and Switchable Access to Functionalized Proteins

(2008) Gonçalo J. L. Bernardes et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Second-Generation Difluorinated Cyclooctynes for Copper-Free Click Chemistry

(2008) Julian A. Codelli et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Tetrazine Ligation: Fast Bioconjugation Based on Inverse-Electron-Demand Diels−Alder Reactivity

(2008) Melissa L. Blackman et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Selective Recognition of Protein Tetraserine Motifs with a Cell-Permeable, Pro-fluorescent Bis-boronic Acid

(2008) Tiffany L. Halo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Cell-surface protein-protein interaction analysis with time-resolved FRET and snap-tag technologies: application to GPCR oligomerization

(2008) Damien Maurel et al.   NATURE METHODS

Imaging the glycome

(2008) S. T. Laughlin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In Vivo Imaging of Membrane-Associated Glycans in Developing Zebrafish

(2008) S. T. Laughlin et al.   SCIENCE

A Submicrogram-Scale Protocol for Biomolecule-Based PET Imaging by Rapid 6π-Azaelectrocyclization: Visualization of Sialic Acid Dependent Circulatory Residence of Glycoproteins

(2007) Katsunori Tanaka et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Target-Specific Chemical Acylation of Lectins by Ligand-Tethered DMAP Catalysts

(2007) Yoichiro Koshi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Construction of a fluorescent biosensor family

(2002) Robert M. De Lorimier et al.   PROTEIN SCIENCE