Peptide Immunoaffinity Enrichment and Targeted Mass Spectrometry Enables Multiplex, Quantitative Pharmacodynamic Studies of Phospho-Signaling

Reproducibility: Standardize antibodies used in research

(2015) Andrew Bradbury et al.   NATURE

An analysis of critical factors for quantitative immunoblotting

(2015) Kevin A. Janes   Science Signaling

An analysis of critical factors for quantitative immunoblotting

(2015) Kevin A. Janes   Science Signaling

Inter-individual variation in DNA repair capacity: A need for multi-pathway functional assays to promote translational DNA repair research

(2014) Zachary D. Nagel et al.   DNA REPAIR

High-Affinity Recombinant Antibody Fragments (Fabs) Can Be Applied in Peptide Enrichment Immuno-MRM Assays

(2014) Jeffrey R. Whiteaker et al.   JOURNAL OF PROTEOME RESEARCH

Isobaric Labeling-Based Relative Quantification in Shotgun Proteomics

(2014) Navin Rauniyar et al.   JOURNAL OF PROTEOME RESEARCH

Off-Line High-pH Reversed-Phase Fractionation for In-Depth Phosphoproteomics

(2014) Tanveer S. Batth et al.   JOURNAL OF PROTEOME RESEARCH

Realizing the Promise of Reverse Phase Protein Arrays for Clinical, Translational, and Basic Research: A Workshop Report

(2014) Rehan Akbani et al.   MOLECULAR & CELLULAR PROTEOMICS

Ischemia in Tumors Induces Early and Sustained Phosphorylation Changes in Stress Kinase Pathways but Does Not Affect Global Protein Levels

(2014) Philipp Mertins et al.   MOLECULAR & CELLULAR PROTEOMICS

Targeted Peptide Measurements in Biology and Medicine: Best Practices for Mass Spectrometry-based Assay Development Using a Fit-for-Purpose Approach

(2014) Steven A. Carr et al.   MOLECULAR & CELLULAR PROTEOMICS

Radiogenomics: Using Genetics to Identify Cancer Patients at Risk for Development of Adverse Effects Following Radiotherapy

(2014) S. L. Kerns et al.   Cancer Discovery

Ultradeep Human Phosphoproteome Reveals a Distinct Regulatory Nature of Tyr and Ser/Thr-Based Signaling

(2014) Kirti Sharma et al.   Cell Reports

Inductively coupled plasma mass spectrometry-based immunoassay: A review

(2013) Rui Liu et al.   MASS SPECTROMETRY REVIEWS

Constrained selected reaction monitoring: Quantification of selected post-translational modifications and protein isoforms

(2013) Xiaoqian Liu et al.   METHODS

Western BlotsversusSelected Reaction Monitoring Assays: Time to Turn the Tables?

(2013) Ruedi Aebersold et al.   MOLECULAR & CELLULAR PROTEOMICS

NIH mulls rules for validating key results

(2013) Meredith Wadman   NATURE

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins

(2013) Jacob J Kennedy et al.   NATURE METHODS

The ATM protein kinase: regulating the cellular response to genotoxic stress and more

(2013) Yosef Shiloh et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Connecting Genomic Alterations to Cancer Biology with Proteomics: The NCI Clinical Proteomic Tumor Analysis Consortium

(2013) M. J. Ellis et al.   Cancer Discovery

PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS

(2012) Matthew P. Stokes et al.   MOLECULAR & CELLULAR PROTEOMICS

Parallel Reaction Monitoring for High Resolution and High Mass Accuracy Quantitative, Targeted Proteomics

(2012) Amelia C. Peterson et al.   MOLECULAR & CELLULAR PROTEOMICS

Raise standards for preclinical cancer research

(2012) C. Glenn Begley et al.   NATURE

Quantitative analysis of peptides and proteins in biomedicine by targeted mass spectrometry

(2012) Michael A Gillette et al.   NATURE METHODS

Proteomics meets the scientific method

(2012) Paola Picotti et al.   NATURE METHODS

Multiplexed quantification of estrogen receptor and HER2/Neu in tissue and cell lysates by peptide immunoaffinity enrichment mass spectrometry

(2012) Regine M. Schoenherr et al.   PROTEOMICS

Sample preparation and analytical strategies for large-scale phosphoproteomics experiments

(2012) Evgeny Kanshin et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

Bio-Rad’s Bio-Plex® suspension array system, xMAP technology overview

(2012) Brett Houser   ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY

ATM and the Molecular Pathogenesis of Ataxia Telangiectasia

(2011) Peter J. McKinnon   Annual Review of Pathology-Mechanisms of Disease

Evaluation of Large Scale Quantitative Proteomic Assay Development Using Peptide Affinity-based Mass Spectrometry

(2011) Jeffrey R. Whiteaker et al.   MOLECULAR & CELLULAR PROTEOMICS

Interlaboratory Evaluation of Automated, Multiplexed Peptide Immunoaffinity Enrichment Coupled to Multiple Reaction Monitoring Mass Spectrometry for Quantifying Proteins in Plasma

(2011) Eric Kuhn et al.   MOLECULAR & CELLULAR PROTEOMICS

Sequential Multiplexed Analyte Quantification Using Peptide Immunoaffinity Enrichment Coupled to Mass Spectrometry

(2011) Jeffrey R. Whiteaker et al.   MOLECULAR & CELLULAR PROTEOMICS

A targeted proteomics–based pipeline for verification of biomarkers in plasma

(2011) Jeffrey R Whiteaker et al.   NATURE BIOTECHNOLOGY

PhosphoSitePlus: a comprehensive resource for investigating the structure and function of experimentally determined post-translational modifications in man and mouse

(2011) P. V. Hornbeck et al.   NUCLEIC ACIDS RESEARCH

Comparative Analysis of Proteome and Transcriptome Variation in Mouse

(2011) Anatole Ghazalpour et al.   PLoS Genetics

Skyline: an open source document editor for creating and analyzing targeted proteomics experiments

(2010) Brendan MacLean et al.   BIOINFORMATICS

The DNA Damage Response: Making It Safe to Play with Knives

(2010) Alberto Ciccia et al.   MOLECULAR CELL

The fundamental flaws of immunoassays and potential solutions using tandem mass spectrometry

(2009) Andrew N. Hoofnagle et al.   JOURNAL OF IMMUNOLOGICAL METHODS

An Automated and Multiplexed Method for High Throughput Peptide Immunoaffinity Enrichment and Multiple Reaction Monitoring Mass Spectrometry-based Quantification of Protein Biomarkers

(2009) Jeffrey R. Whiteaker et al.   MOLECULAR & CELLULAR PROTEOMICS

The DNA-damage response in human biology and disease

(2009) Stephen P. Jackson et al.   NATURE

Multi-site assessment of the precision and reproducibility of multiple reaction monitoring–based measurements of proteins in plasma

(2009) Terri A Addona et al.   NATURE BIOTECHNOLOGY

Selected reaction monitoring for quantitative proteomics: a tutorial

(2008) Vinzenz Lange et al.   Molecular Systems Biology