Organoid-on-a-chip: Current challenges, trends, and future scope toward medicine

In Vitro three-dimensional (3D) cell culture tools for spheroid and organoid models

(2023) Sang-Yun Lee et al.   SLAS Discovery

Integrated-gut-liver-on-a-chip platform as an in vitro human model of non-alcoholic fatty liver disease

(2023) Jiandong Yang et al.   Communications Biology

Current microfluidic platforms for reverse engineering of cornea

(2023) Qinyu Li et al.   Materials Today Bio

A Biomimetic Human Lung‐on‐a‐Chip with Colorful Display of Microphysiological Breath

(2022) Yujuan Zhu et al.   ADVANCED MATERIALS

Human organoid models to study SARS-CoV-2 infection

(2022) Yuling Han et al.   NATURE METHODS

Generation of vascularized brain organoids to study neurovascular interactions

(2022) Xin-Yao Sun et al.   eLife

Skin-on-a-Chip Technology: Microengineering Physiologically Relevant In Vitro Skin Models

(2022) Patrícia Zoio et al.   Pharmaceutics

A multi-organ chip with matured tissue niches linked by vascular flow

(2022) Kacey Ronaldson-Bouchard et al.   Nature Biomedical Engineering

Multiorgan‐on‐a‐chip for realization of gut‐skin axis

(2022) Hye Ri Lee et al.   BIOTECHNOLOGY AND BIOENGINEERING

Perspectives on Microfluidics for the Study of Asphaltenes in Upstream Hydrocarbon Production: A Minireview

(2022) Mrityunjay K. Sharma et al.   ENERGY & FUELS

Adaptable Microfluidic Vessel-on-a-Chip Platform for Investigating Tumor Metastatic Transport in Bloodstream

(2022) Yue Wu et al.   ANALYTICAL CHEMISTRY

SARS-CoV-2 infects human brain organoids causing cell death and loss of synapses that can be rescued by treatment with Sofosbuvir

(2022) Pinar Mesci et al.   PLOS BIOLOGY

New Strategy for Promoting Vascularization in Tumor Spheroids in a Microfluidic Assay

(2022) Zhengpeng Wan et al.   Advanced Healthcare Materials

Accelerated and Improved Differentiation of Retinal Organoids from Pluripotent Stem Cells in Rotating-Wall Vessel Bioreactors

(2021) Tyler J. DiStefano et al.   Stem Cell Reports

Review of 3D-Printed functionalized devices for chemical and biochemical analysis

(2021) Cheng-Kuan Su   ANALYTICA CHIMICA ACTA

Accuracy of Using a Patient-Derived Tumor Organoid Culture Model to Predict the Response to Chemotherapy Regimens In Stage IV Colorectal Cancer

(2021) Ting Wang et al.   DISEASES OF THE COLON & RECTUM

Rapid Manufacturing of Multilayered Microfluidic Devices for Organ on a Chip Applications

(2021) Roberto Paoli et al.   SENSORS

Organoid microphysiological system preserves pancreatic islet function within 3D matrix

(2021) S. N. Patel et al.   Science Advances

Mechanical plasticity of collagen directs branch elongation in human mammary gland organoids

(2021) B. Buchmann et al.   Nature Communications

Microfluidic droplets as structural templates for Matrigel to enable 1-week large organoid modeling

(2021) Weijie Zhang et al.   CHEMICAL ENGINEERING SCIENCE

A Gut-Brain Axis-on-a-Chip for studying transport across epithelial and endothelial barriers

(2021) Min-Hyeok Kim et al.   JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY

Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids

(2021) Ann-Na Cho et al.   Nature Communications

Dynamic persistence of UPEC intracellular bacterial communities in a human bladder-chip model of urinary tract infection

(2021) Kunal Sharma et al.   eLife

Mammary Tumor Organoid Culture in Non‐Adhesive Alginate for Luminal Mechanics and High‐Throughput Drug Screening

(2021) Guocheng Fang et al.   Advanced Science

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

(2021) Xuewei Zhang et al.   Bioactive Materials

Organ-on-a-chip systems for vascular biology

(2021) Christian J. Mandrycky et al.   JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY

Low-cost rapid prototyping and assembly of an open microfluidic device for a 3D vascularized organ-on-a-chip

(2021) Qinyu Li et al.   LAB ON A CHIP

Patient-Derived Organoids in Precision Medicine: Drug Screening, Organoid-on-a-Chip and Living Organoid Biobank

(2021) Zilong Zhou et al.   Frontiers in Oncology

Towards organoid culture without Matrigel

(2021) Mark T. Kozlowski et al.   Communications Biology

Liver‐on‐a‐Chip Models of Fatty Liver Disease

(2020) Shabir Hassan et al.   HEPATOLOGY

Characterization of a heparinized decellularized scaffold and its effects on mechanical and structural properties

(2020) Ji Li et al.   JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION

In Vitro Biocompatibility of Decellularized Cultured Plant Cell-Derived Matrices

(2020) Nhi V. Phan et al.   ACS Biomaterials-Science & Engineering

Quantitative prediction of human pharmacokinetic responses to drugs via fluidically coupled vascularized organ chips

(2020) Anna Herland et al.   Nature Biomedical Engineering

Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2

(2020) Vanessa Monteil et al.   CELL

Patient-derived tumor organoid predicts drugs response in glioblastoma: A step forward in personalized cancer therapy?

(2020) Herbert H-F. Loong et al.   JOURNAL OF CLINICAL NEUROSCIENCE

Infection of bat and human intestinal organoids by SARS-CoV-2

(2020) Jie Zhou et al.   NATURE MEDICINE

Human‐on‐Leaf‐Chip: A Biomimetic Vascular System Integrated with Chamber‐Specific Organs

(2020) Mao Mao et al.   Small

A Droplet Microfluidic System to Fabricate Hybrid Capsules Enabling Stem Cell Organoid Engineering

(2020) Haitao Liu et al.   Advanced Science

One-Step Generation of Core-Shell Gelatin Methacrylate (GelMA) Microgels Using a Droplet Microfluidic System

(2019) Hui Wang et al.   Advanced Materials Technologies

Engineering Microfluidic Organoid-on-a-Chip Platforms

(2019) Fang Yu et al.   Micromachines

Cerebral organoids at the air–liquid interface generate diverse nerve tracts with functional output

(2019) Stefano L. Giandomenico et al.   NATURE NEUROSCIENCE

A bioprinted human-glioblastoma-on-a-chip for the identification of patient-specific responses to chemoradiotherapy

(2019) Hee-Gyeong Yi et al.   Nature Biomedical Engineering

An organoid platform for ovarian cancer captures intra- and interpatient heterogeneity

(2019) Oded Kopper et al.   NATURE MEDICINE

Human iPSC-Derived Blood-Brain Barrier Chips Enable Disease Modeling and Personalized Medicine Applications

(2019) Gad D. Vatine et al.   Cell Stem Cell

In vitro humanized 3D microfluidic chip for testing personalized immunotherapeutics for head and neck cancer patients

(2019) Ahmed Al-Samadi et al.   EXPERIMENTAL CELL RESEARCH

Liver organoids: from basic research to therapeutic applications

(2019) Nicole Prior et al.   GUT

An innovative method to obtain porous porcine aorta scaffolds for tissue engineering

(2019) Xiaohong Liu et al.   ARTIFICIAL ORGANS

Recent Trends in Decellularized Extracellular Matrix Bioinks for 3D Printing: An Updated Review

(2019) Kevin Dzobo et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Multiscale reverse engineering of the human ocular surface

(2019) Jeongyun Seo et al.   NATURE MEDICINE

All Together Now: Modeling the Interaction of Neural With Non-neural Systems Using Organoid Models

(2019) Evelyn Chukwurah et al.   Frontiers in Neuroscience

Engineering of human brain organoids with a functional vascular-like system

(2019) Bilal Cakir et al.   NATURE METHODS

A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier

(2019) Astgik Petrosyan et al.   Nature Communications

Vascularized cancer on a chip: The effect of perfusion on growth and drug delivery of tumor spheroid

(2019) Yuji Nashimoto et al.   BIOMATERIALS

Proteomic analysis of decellularized pancreatic matrix identifies collagen V as a critical regulator for islet organogenesis from human pluripotent stem cells

(2019) Huanjing Bi et al.   BIOMATERIALS

Organoids from the Human Fetal and Adult Pancreas

(2019) Jeetindra R. A. Balak et al.   Current Diabetes Reports

Novel Sodium Deoxycholate-Based Chemical Decellularization Method for Peripheral Nerve

(2019) Michaela W. McCrary et al.   TISSUE ENGINEERING PART C-METHODS

Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

(2019) Giovanni Giuseppe Giobbe et al.   Nature Communications

Injectable degradable PVA microgels prepared by microfluidic technology for controlled osteogenic differentiation of mesenchymal stem cells

(2018) Yong Hou et al.   Acta Biomaterialia

Progress, obstacles, and limitations in the use of stem cells in organ-on-a-chip models

(2018) Alexa Wnorowski et al.   ADVANCED DRUG DELIVERY REVIEWS

Feasibility and acceptability of combining cognitive behavioural therapy techniques with swallowing therapy in head and neck cancer dysphagia

(2018) J. M. Patterson et al.   BMC CANCER

A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity

(2018) Norman Sachs et al.   CELL

Scalable Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in 3D

(2018) Li Yan et al.   International Journal of Biological Sciences

Fundamentals of rapid injection molding for microfluidic cell-based assays

(2018) Ulri N. Lee et al.   LAB ON A CHIP

Microfluidics within a well: an injection-molded plastic array 3D culture platform

(2018) Younggyun Lee et al.   LAB ON A CHIP

A microengineered human corneal epithelium-on-a-chip for eye drops mass transport evaluation

(2018) Devasier Bennet et al.   LAB ON A CHIP

A multiplexed microfluidic system for evaluation of dynamics of immune–tumor interactions

(2018) N. Moore et al.   LAB ON A CHIP

Patient-derived organoids model treatment response of metastatic gastrointestinal cancers

(2018) Georgios Vlachogiannis et al.   SCIENCE

A Simple Bioreactor-Based Method to Generate Kidney Organoids from Pluripotent Stem Cells

(2018) Aneta Przepiorski et al.   Stem Cell Reports

Investigation of the effect of hepatic metabolism on off-target cardiotoxicity in a multi-organ human-on-a-chip system

(2018) Carlota Oleaga et al.   BIOMATERIALS

Rapid 3D bioprinting of decellularized extracellular matrix with regionally varied mechanical properties and biomimetic microarchitecture

(2018) Xuanyi Ma et al.   BIOMATERIALS

Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids

(2018) Krijn K. Dijkstra et al.   CELL

Organoid technology and applications in cancer research

(2018) Hanxiao Xu et al.   Journal of Hematology & Oncology

A linked organ-on-chip model of the human neurovascular unit reveals the metabolic coupling of endothelial and neuronal cells

(2018) Ben M Maoz et al.   NATURE BIOTECHNOLOGY

Optimizing Process Parameters in Commercial Micro-Stereolithography for Forming Emulsions and Polymer Microparticles in Nonplanar Microfluidic Devices

(2018) Max J. Männel et al.   Advanced Materials Technologies

In situ generation of human brain organoids on a micropillar array

(2017) Yujuan Zhu et al.   LAB ON A CHIP

A disease model of diabetic nephropathy in a glomerulus-on-a-chip microdevice

(2017) Li Wang et al.   LAB ON A CHIP

Mimicking arterial thrombosis in a 3D-printed microfluidic in vitro vascular model based on computed tomography angiography data

(2017) Pedro F. Costa et al.   LAB ON A CHIP

Synthetic hydrogels mimicking basement membrane matrices to promote cell-matrix interactions

(2017) Ricardo Cruz-Acuña et al.   MATRIX BIOLOGY

Cell diversity and network dynamics in photosensitive human brain organoids

(2017) Giorgia Quadrato et al.   NATURE

Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium

(2017) Margherita Y. Turco et al.   NATURE CELL BIOLOGY

Mechanical forces direct stem cell behaviour in development and regeneration

(2017) Kyle H. Vining et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors

(2017) Yu Shrike Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids

(2017) Russell W. Jenkins et al.   Cancer Discovery

Tailoring mechanical properties of decellularized extracellular matrix bioink by vitamin B2-induced photo-crosslinking

(2016) Jinah Jang et al.   Acta Biomaterialia

Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip

(2016) Yu Shrike Zhang et al.   BIOMATERIALS

Innovative design of reel-to-reel hot embossing system for production of plastic microlens array films

(2016) Chih-Yuan Chang et al.   INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY

The impact of decellularization agents on renal tissue extracellular matrix

(2016) Nafiseh Poornejad et al.   JOURNAL OF BIOMATERIALS APPLICATIONS

Biological functionality and mechanistic contribution of extracellular matrix-ornamented three dimensional Ti-6Al-4V mesh scaffolds

(2016) A. Kumar et al.   JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A

Translation of Human-Induced Pluripotent Stem Cells

(2016) Nazish Sayed et al.   JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY

Beating heart on a chip: a novel microfluidic platform to generate functional 3D cardiac microtissues

(2016) Anna Marsano et al.   LAB ON A CHIP

Brain-on-a-chip model enables analysis of human neuronal differentiation and chemotaxis

(2016) Onur Kilic et al.   LAB ON A CHIP

Controlled assembly of heterotypic cells in a core–shell scaffold: organ in a droplet

(2016) Qiushui Chen et al.   LAB ON A CHIP

Modular, pumpless body-on-a-chip platform for the co-culture of GI tract epithelium and 3D primary liver tissue

(2016) Mandy B. Esch et al.   LAB ON A CHIP

A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC)

(2016) Kevin Alessandri et al.   LAB ON A CHIP

Engineering anastomosis between living capillary networks and endothelial cell-lined microfluidic channels

(2016) Xiaolin Wang et al.   LAB ON A CHIP

Experimental study of polymer microlens fabrication using partial-filling hot embossing technique

(2016) Sean Moore et al.   MICROELECTRONIC ENGINEERING

Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing

(2016) Johan U. Lind et al.   NATURE MATERIALS

Induced pluripotent stem cells: at the heart of cardiovascular precision medicine

(2016) Ian Y. Chen et al.   Nature Reviews Cardiology

Astrocyte Differentiation of Human Pluripotent Stem Cells: New Tools for Neurological Disorder Research

(2016) Abinaya Chandrasekaran et al.   Frontiers in Cellular Neuroscience

A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells

(2016) Jing Kong et al.   Oncotarget

Investigating Nonalcoholic Fatty Liver Disease in a Liver-on-a-Chip Microfluidic Device

(2016) Manuele Gori et al.   PLoS One

3D microtumors in vitro supported by perfused vascular networks

(2016) Agua Sobrino et al.   Scientific Reports

Tailoring Material Properties of Cardiac Matrix Hydrogels To Induce Endothelial Differentiation of Human Mesenchymal Stem Cells

(2015) Megan E. Jeffords et al.   ACS Applied Materials & Interfaces

3D printing of soft lithography mold for rapid production of polydimethylsiloxane-based microfluidic devices for cell stimulation with concentration gradients

(2015) Ken-ichiro Kamei et al.   BIOMEDICAL MICRODEVICES

Islet Neogenesis Associated Protein (INGAP) induces the differentiation of an adult human pancreatic ductal cell line into insulin-expressing cells through stepwise activation of key transcription factors for embryonic beta cell development

(2015) Béatrice Assouline-Thomas et al.   DIFFERENTIATION

A multi-organ chip co-culture of neurospheres and liver equivalents for long-term substance testing

(2015) Eva-Maria Materne et al.   JOURNAL OF BIOTECHNOLOGY

A basic experimental study of ultrasonic assisted hot embossing process for rapid fabrication of microlens arrays

(2015) Chih-Yuan Chang et al.   JOURNAL OF MICROMECHANICS AND MICROENGINEERING

3D liver models on a microplatform: well-defined culture, engineering of liver tissue and liver-on-a-chip

(2015) Da Yoon No et al.   LAB ON A CHIP

A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents

(2015) Ilka Maschmeyer et al.   LAB ON A CHIP

A lung-on-a-chip array with an integrated bio-inspired respiration mechanism

(2015) Andreas O. Stucki et al.   LAB ON A CHIP

Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis

(2015) Minoru Takasato et al.   NATURE

Preserved genetic diversity in organoids cultured from biopsies of human colorectal cancer metastases

(2015) Fleur Weeber et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

3D printed molds for non-planar PDMS microfluidic channels

(2015) Yongha Hwang et al.   SENSORS AND ACTUATORS A-PHYSICAL

Micro structure fabrication with a simplified hot embossing method

(2015) Yong He et al.   RSC Advances

Simple 3D Printed Scaffold-Removal Method for the Fabrication of Intricate Microfluidic Devices

(2015) Vittorio Saggiomo et al.   Advanced Science

Matrigel: From discovery and ECM mimicry to assays and models for cancer research

(2014) Gabriel Benton et al.   ADVANCED DRUG DELIVERY REVIEWS

Proteome array identification of bioactive soluble proteins/peptides in Matrigel: relevance to stem cell responses

(2014) Neil C. Talbot et al.   CYTOTECHNOLOGY

Mold filling analysis of an alignment structure in micro hot embossing

(2014) Juan A. Gomez et al.   FIBERS AND POLYMERS

Development of rapid tooling by rapid prototyping technology and electroless nickel plating for low-volume production of plastic parts

(2014) Janaka Rajaguru et al.   INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY

Mail-order microfluidics: evaluation of stereolithography for the production of microfluidic devices

(2014) Anthony K. Au et al.   LAB ON A CHIP

Fabrication and characterization of polymer microprisms

(2014) William Brian Derek Forfang et al.   MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS

Fluid shear stress threshold regulates angiogenic sprouting

(2014) P. A. Galie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Organogenesis in a dish: Modeling development and disease using organoid technologies

(2014) M. A. Lancaster et al.   SCIENCE

From Bench to Cageside: Risk Assessment for Rodent Pathogen Contamination of Cells and Biologics

(2013) N. C. Peterson   ILAR JOURNAL

Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment

(2013) Kyung-Jin Jang et al.   Integrative Biology

Microfluidic heart on a chip for higher throughput pharmacological studies

(2013) Ashutosh Agarwal et al.   LAB ON A CHIP

Rapid fabrication of nickel molds for prototyping embossed plastic microfluidic devices

(2013) Richard Novak et al.   LAB ON A CHIP

Liver-cell patterning Lab Chip: mimicking the morphology of liver lobule tissue

(2013) Chen-Ta Ho et al.   LAB ON A CHIP

Vascularized and functional human liver from an iPSC-derived organ bud transplant

(2013) Takanori Takebe et al.   NATURE

Cerebral organoids model human brain development and microcephaly

(2013) Madeline A. Lancaster et al.   NATURE

Drop formation in non-planar microfluidic devices

(2012) Assaf Rotem et al.   LAB ON A CHIP

Characterization of a microfluidic in vitro model of the blood-brain barrier (μBBB)

(2012) Ross Booth et al.   LAB ON A CHIP

Construction of oxygen and chemical concentration gradients in a single microfluidic device for studying tumor cell–drug interactions in a dynamic hypoxia microenvironment

(2012) Lei Wang et al.   LAB ON A CHIP

Development and Evolution of the Human Neocortex

(2011) Jan H. Lui et al.   CELL

Removal of lactate dehydrogenase-elevating virus from human-in-mouse breast tumor xenografts by cell-sorting

(2011) Huiping Liu et al.   JOURNAL OF VIROLOGICAL METHODS

Axon diodes for the reconstruction of oriented neuronal networks in microfluidic chambers

(2011) Jean-Michel Peyrin et al.   LAB ON A CHIP

Hot embossing at viscous state to enhance filling process for complex polymer structures

(2011) Xuelin Zhu et al.   MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS

Mechanical control of tissue and organ development

(2010) T. Mammoto et al.   DEVELOPMENT

Microfabrication of a nickel mold insert by a modified deep X-ray lithography process and its application to hot embossing

(2010) Bong-Kee Lee et al.   MICROELECTRONIC ENGINEERING

Low-temperature embossing technique for fabrication of large-area polymeric microlens array with supercritical carbon dioxide

(2010) Jing-Tang Wu et al.   MICROELECTRONIC ENGINEERING

Matrigel: A complex protein mixture required for optimal growth of cell culture

(2010) Chris S. Hughes et al.   PROTEOMICS

Reconstituting Organ-Level Lung Functions on a Chip

(2010) D. Huh et al.   SCIENCE

Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells

(2009) Donghui Zhang et al.   CELL RESEARCH

Investigation of hot roller embossing for microfluidic devices

(2009) L P Yeo et al.   JOURNAL OF MICROMECHANICS AND MICROENGINEERING

The elastic modulus of Matrigel™ as determined by atomic force microscopy

(2009) Shauheen S. Soofi et al.   JOURNAL OF STRUCTURAL BIOLOGY

A micro cell culture analog (µCCA) with 3-D hydrogel culture of multiple cell lines to assess metabolism-dependent cytotoxicity of anti-cancer drugs

(2009) Jong Hwan Sung et al.   LAB ON A CHIP

Biological implications of polydimethylsiloxane-based microfluidic cell culture

(2009) Keil J. Regehr et al.   LAB ON A CHIP

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Lactate Dehydrogenase-Elevating Virus Induces Systemic Lymphocyte Activation via TLR7-Dependent IFNα Responses by Plasmacytoid Dendritic Cells

(2009) Christoph G. Ammann et al.   PLoS One

Micro-fabrication of polymeric devices using hot roller embossing

(2008) Lip Pin Yeo et al.   MICROELECTRONIC ENGINEERING