Several Affinity Tags Commonly Used in Chromatographic Purification

The structure of the SBP-Tag–streptavidin complex reveals a novel helical scaffold bridging binding pockets on separate subunits

(2013) Isabelle H. Barrette-Ng et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Large scale modification of biomolecules using immobilized sortase A from Staphylococcus aureus

(2013) Max Steinhagen et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Development of the Twin-Strep-tag® and its application for purification of recombinant proteins from cell culture supernatants

(2013) Thomas G.M. Schmidt et al.   PROTEIN EXPRESSION AND PURIFICATION

Presence and removal of a contaminating NADH oxidation activity in recombinant maltose-binding protein fusion proteins expressed in Escherichia coli

(2012) Fengguang Guo et al.   BIOTECHNIQUES

A new idea for simple and rapid monitoring of gene expression: requirement of nucleotide sequences encoding an N-terminal HA tag in the T7 promoter-driven expression in E. coli

(2012) Jeong-Mi Moon et al.   BIOTECHNOLOGY LETTERS

Quantitative Proteomics for Cancer Biomarker Discovery

(2012) Shufang Liang et al.   COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

Identification of Protein Interacting Partners Using Tandem Affinity Purification

(2012) Dalan Bailey et al.   Jove-Journal of Visualized Experiments

Expression of proteins inEscherichia colias fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline

(2011) Stephen N. Hewitt et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

Self-cleaving fusion tags for recombinant protein production

(2011) Yifeng Li   BIOTECHNOLOGY LETTERS

The tandem affinity purification technology: an overview

(2011) Yifeng Li   BIOTECHNOLOGY LETTERS

Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation

(2011) Tracy Keates et al.   New Biotechnology

An overview of enzymatic reagents for the removal of affinity tags

(2011) David S. Waugh   PROTEIN EXPRESSION AND PURIFICATION

Interaction proteomics: characterization of protein complexes using tandem affinity purification–mass spectrometry: Figure 1

(2010) Pamela Völkel et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Commonly used tag combinations for tandem affinity purification

(2010) Yifeng Li   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

The potential role of self-cleaving purification tags in commercial-scale processes

(2010) Baley A. Fong et al.   TRENDS IN BIOTECHNOLOGY

Affinity Purification Combined with Mass Spectrometry-Based Proteomic Strategy to Study Mammalian Protein Complex and Protein-Protein Interactions

(2009) Shufang Liang et al.   Current Proteomics

Analysis of the protein complex associated with 14-3-3 epsilon by a deuterated-leucine labeling quantitative proteomics strategy

(2009) Shufang Liang et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Utilization of Arg-elution method for FLAG-tag based chromatography

(2009) Mutsumi Futatsumori-Sugai et al.   PROTEIN EXPRESSION AND PURIFICATION

Prokaryotic expression, purification of a new tumor-relative protein FAM92A1-289 and its characterization in renal cell carcinoma

(2008) Shufang Liang et al.   CANCER LETTERS

Enhancing the specificity of the enterokinase cleavage reaction to promote efficient cleavage of a fusion tag

(2008) S. Hesam Shahravan et al.   PROTEIN EXPRESSION AND PURIFICATION

Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli

(2002) Martin Hammarström et al.   PROTEIN SCIENCE