Bringing functions together with fusion enzymes—from nature’s inventions to biotechnological applications

Enzymatic Degradation of (Ligno)cellulose

(2014) Uwe Bornscheuer et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Synergistic proteins for the enhanced enzymatic hydrolysis of cellulose by cellulase

(2014) In Jung Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Synergism of cellulase, xylanase, and pectinase on hydrolyzing sugarcane bagasse resulting from different pretreatment technologies

(2014) Jingbo Li et al.   BIORESOURCE TECHNOLOGY

Generating bifunctional fusion enzymes composed of heat-active endoglucanase (Cel5A) and endoxylanase (XylT)

(2014) Mazen Rizk et al.   BIOTECHNOLOGY LETTERS

Extremozymes—biocatalysts with unique properties from extremophilic microorganisms

(2014) Skander Elleuche et al.   CURRENT OPINION IN BIOTECHNOLOGY

A universal cloning method based on yeast homologous recombination that is simple, efficient, and versatile

(2014) Tammy M. Joska et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Design and establishment of a vector system that enables production of multifusion proteins and easy purification by a two-step affinity chromatography approach

(2014) Tabea Marquardt et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Split-gene system for hybrid wheat seed production

(2014) K. Kempe et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Distinct Model of Synergism between a Processive Endocellulase (TfCel9A) and an Exocellulase (TfCel48A) from Thermobifida fusca

(2013) Maxim Kostylev et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Addition of a carbohydrate-binding module enhances cellulase penetration into cellulose substrates

(2013) Vimalier Reyes-Ortiz et al.   Biotechnology for Biofuels

The synergistic action of accessory enzymes enhances the hydrolytic potential of a “cellulase mixture” but is highly substrate specific

(2013) Jinguang Hu et al.   Biotechnology for Biofuels

Selecting β-glucosidases to support cellulases in cellulose saccharification

(2013) Hele Teugjas et al.   Biotechnology for Biofuels

Construction of a chimeric thermoacidophilic beta-endoglucanase

(2013) Kristina Kufner et al.   BMC BIOCHEMISTRY

Enzymatic properties of Thermoanaerobacterium thermosaccharolyticum β-glucosidase fused to Clostridium cellulovorans cellulose binding domain and its application in hydrolysis of microcrystalline cellulose

(2013) Linguo Zhao et al.   BMC BIOTECHNOLOGY

A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector

(2013) Raquel Buj et al.   BMC MOLECULAR BIOLOGY

Evaluation of immobilized enzymes for industrial applications

(2013) Andreas Liese et al.   CHEMICAL SOCIETY REVIEWS

Fusion of cellulose binding domain from Trichoderma reesei CBHI to Cryptococcus sp. S-2 cellulase enhances its binding affinity and its cellulolytic activity to insoluble cellulosic substrates

(2013) Jantaporn Thongekkaew et al.   ENZYME AND MICROBIAL TECHNOLOGY

Golden GATEway Cloning – A Combinatorial Approach to Generate Fusion and Recombination Constructs

(2013) Stephan Kirchmaier et al.   PLoS One

Inteins: nature's gift to protein chemists

(2013) Neel H. Shah et al.   Chemical Science

Specific Fusion of β-1,4-Endoglucanase and β-1,4-Glucosidase Enhances Cellulolytic Activity and Helps in Channeling of Intermediates

(2012) Nidhi Adlakha et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

End-to-end gene fusions and their impact on the production of multifunctional biomass degrading enzymes

(2012) Mazen Rizk et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Improved lignocellulose conversion to biofuels with thermophilic bacteria and thermostable enzymes

(2012) Aditya Bhalla et al.   BIORESOURCE TECHNOLOGY

Recent progress in intein research: from mechanism to directed evolution and applications

(2012) Gerrit Volkmann et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Characterisation of Two Bifunctional Cellulase–Xylanase Enzymes Isolated from a Bovine Rumen Metagenome Library

(2012) K. J. Rashamuse et al.   CURRENT MICROBIOLOGY

Engineering a thermoregulated intein-modified xylanase into maize for consolidated lignocellulosic biomass processing

(2012) Binzhang Shen et al.   NATURE BIOTECHNOLOGY

Preparation of unnatural N-to-N and C-to-C protein fusions

(2012) Martin D. Witte et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Introducing endo-xylanase activity into an exo-acting arabinofuranosidase that targets side chains

(2012) L. S. McKee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Deconstruction of Lignocellulose into Soluble Sugars by Native and Designer Cellulosomes

(2012) S. Morais et al.   mBio

Synthesis and Characterization of Chimeric Proteins Based on Cellulase and Xylanase from an Insect Gut Bacterium

(2011) Nidhi Adlakha et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Fusion of the OsmC domain from esterase EstO confers thermolability to the cold-active xylanase Xyn8 from Pseudoalteromonas arctica

(2011) Skander Elleuche et al.   EXTREMOPHILES

Engineering Bifunctional Laccase-Xylanase Chimeras for Improved Catalytic Performance

(2011) Lucas F. Ribeiro et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Stabilization by Fusion to the C-terminus of Hyperthermophile Sulfolobus tokodaii RNase HI: A Possibility of Protein Stabilization Tag

(2011) Kazufumi Takano et al.   PLoS One

Inteins, valuable genetic elements in molecular biology and biotechnology

(2010) Skander Elleuche et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Construction and characterization of different fusion proteins between cellulases and β-glucosidase to improve glucose production and thermostability

(2010) Hsiao-Lin Lee et al.   BIORESOURCE TECHNOLOGY

Binding modules alter the activity of chimeric cellulases: Effects of biomass pretreatment and enzyme source

(2010) Tae-Wan Kim et al.   BIOTECHNOLOGY AND BIOENGINEERING

Stability of a guest protein depends on stability of a host protein in insertional fusion

(2010) Brennal Pierre et al.   BIOTECHNOLOGY AND BIOENGINEERING

Multimeric Hemicellulases Facilitate Biomass Conversion

(2009) Z. Fan et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Carbohydrate-binding domains: multiplicity of biological roles

(2009) Daniel Guillén et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Construction, expression and characterization of fusion enzyme from Arthrobacter oxydans dextranase and Klebsiella pneumoniae amylase

(2009) Young-Min Kim et al.   BIOTECHNOLOGY LETTERS

‘Cradle-to-grave’ assessment of existing lignocellulose pretreatment technologies

(2009) Leonardo da Costa Sousa et al.   CURRENT OPINION IN BIOTECHNOLOGY

You're one in a googol: optimizing genes for protein expression

(2009) M. Welch et al.   Journal of the Royal Society Interface

Gemini, a Bifunctional Enzymatic and Fluorescent Reporter of Gene Expression

(2009) Lance Martin et al.   PLoS One

Fluorescent proteins: a cell biologist's user guide

(2009) Erik Lee Snapp   TRENDS IN CELL BIOLOGY

Bifunctional enhancement of a β-glucanase-xylanase fusion enzyme by optimization of peptide linkers

(2008) Ping Lu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Production, purification, and characterization of a fusion protein of carbonic anhydrase fromNeisseria gonorrhoeaeand cellulose binding domain fromClostridium thermocellum

(2008) Zhu Liu et al.   BIOTECHNOLOGY PROGRESS

Engineering the spatial organization of metabolic enzymes: mimicking nature's synergy

(2008) Robert J Conrado et al.   CURRENT OPINION IN BIOTECHNOLOGY

Bifunctional xylanases and their potential use in biotechnology

(2008) Rakhee Khandeparker et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One

Reversible Compartmentalization of de Novo Purine Biosynthetic Complexes in Living Cells

(2008) S. An et al.   SCIENCE