Recent progress in intein research: from mechanism to directed evolution and applications

The Thermococcus kodakaraensis Tko CDC21-1 Intein Activates Its N-Terminal Splice Junction in the Absence of a Conserved Histidine by a Compensatory Mechanism

(2012) Kazuo Tori et al.   BIOCHEMISTRY

Split-intein mediated re-assembly of genetically encoded Ca2+ indicators

(2012) Stanley S.C. Wong et al.   CELL CALCIUM

NMR and Crystal Structures of the Pyrococcus horikoshii RadA Intein Guide a Strategy for Engineering a Highly Efficient and Promiscuous Intein

(2012) Jesper S. Oeemig et al.   JOURNAL OF MOLECULAR BIOLOGY

In-Cell Fluorescence Activation and Labeling of Proteins Mediated by FRET-Quenched Split Inteins

(2012) Radhika Borra et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ultrafast Protein Splicing is Common among Cyanobacterial Split Inteins: Implications for Protein Engineering

(2012) Neel H. Shah et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Development of a novel DnaE intein-based assay for quantitative analysis of G-protein-coupled receptor internalization

(2011) Yaping Zhang et al.   ANALYTICAL BIOCHEMISTRY

Photocontrol of Protein Activity Mediated by the Cleavage Reaction of a Split Intein

(2011) Jens Binschik et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Kinetic Control of One-Pot Trans-Splicing Reactions by Using a Wild-Type and Designed Split Intein

(2011) Neel H. Shah et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Probing Intein-Catalyzed Thioester Formation by Unnatural Amino Acid Substitutions in the Active Site

(2011) Dirk Schwarzer et al.   BIOCHEMISTRY

Branched Intermediate Formation Is the Slowest Step in the Protein Splicing Reaction of the Ala1 KlbA Intein fromMethanococcus jannaschii

(2011) Lana Saleh et al.   BIOCHEMISTRY

Directed Evolution of a Small-Molecule-Triggered Intein with Improved Splicing Properties in Mammalian Cells

(2011) Sun H. Peck et al.   CHEMISTRY & BIOLOGY

Intein lacking conserved C-terminal motif G retains controllable N-cleavage activity

(2011) Gerrit Volkmann et al.   FEBS Journal

Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins inEscherichia coli

(2011) Simo Ellilä et al.   FEBS LETTERS

Split Inteins: Nature’s Protein Ligases

(2011) Neel H. Shah et al.   ISRAEL JOURNAL OF CHEMISTRY

Expanding the Definition of Class 3 Inteins and Their Proposed Phage Origin

(2011) K. Tori et al.   JOURNAL OF BACTERIOLOGY

Structural and Mutational Studies of a Hyperthermophilic Intein from DNA Polymerase II ofPyrococcus abyssi

(2011) Zhenming Du et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Highly Efficient and More Generalcis- andtrans-Splicing Inteins through Sequential Directed Evolution

(2011) Julia H. Appleby-Tagoe et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage

(2011) Wei Lu et al.   JOURNAL OF CHROMATOGRAPHY A

pKaCoupling at the Intein Active Site: Implications for the Coordination Mechanism of Protein Splicing with a Conserved Aspartate

(2011) Zhenming Du et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

An intein-cassette integration approach used for the generation of a split TEV protease activated by conditional protein splicing

(2011) Tim Sonntag et al.   Molecular BioSystems

Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications

(2011) Brian P Callahan et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Inteins, valuable genetic elements in molecular biology and biotechnology

(2010) Skander Elleuche et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Biological Applications of Protein Splicing

(2010) Miquel Vila-Perelló et al.   CELL

Photomodulation of Protein Trans-Splicing Through Backbone Photocaging of the DnaE Split Intein

(2010) Luis Berrade et al.   CHEMBIOCHEM

Canonical Protein Splicing of a Class 1 Intein That Has a Class 3 Noncanonical Sequence Motif

(2010) J. N. Reitter et al.   JOURNAL OF BACTERIOLOGY

Backbone Dynamics and Global Effects of an Activating Mutation in Minimized Mtu RecA Inteins

(2010) Zhenming Du et al.   JOURNAL OF MOLECULAR BIOLOGY

Spontaneous Proton Transfer to a Conserved Intein Residue Determines On-Pathway Protein Splicing

(2010) Brian Pereira et al.   JOURNAL OF MOLECULAR BIOLOGY

Protein immobilization on liposomes and lipid-coated nanoparticles by protein trans-splicing

(2010) Nam Ky Chu et al.   JOURNAL OF PEPTIDE SCIENCE

Protein trans-splicing and its use in structural biology: opportunities and limitations

(2010) Gerrit Volkmann et al.   Molecular BioSystems

Branched intermediate formation stimulates peptide bond cleavage in protein splicing

(2010) Silvia Frutos et al.   Nature Chemical Biology

Peptide and protein thioester synthesis via N→S acyl transfer

(2010) Jaskiranjit Kang et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

The Deinococcus radiodurans Snf2 intein caught in the act: Detection of the Class 3 intein signature Block F branched intermediate

(2010) Lear E. Brace et al.   PROTEIN SCIENCE

“Splicing up” drug discovery.☆Cell-based expression and screening of genetically-encoded libraries of backbone-cyclized polypeptides

(2009) Harshkumar Sancheti et al.   ADVANCED DRUG DELIVERY REVIEWS

Protein splicing: A versatile tool for drug discovery☆

(2009) Manoj Cheriyan et al.   ADVANCED DRUG DELIVERY REVIEWS

Segmental Isotopic Labeling of a Central Domain in a Multidomain Protein by ProteinTrans-Splicing Using Only One Robust DnaE Intein

(2009) Alena E. L. Busche et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Expanding the Scope of ProteinTrans-Splicing to Fragment Ligation of an Integral Membrane Protein: Towards Modulation of Porin-Based Ion Channels by Chemical Modification

(2009) Steffen Brenzel et al.   CHEMBIOCHEM

Split Inteins as Versatile Tools for Protein Semisynthesis

(2009) Henning D. Mootz   CHEMBIOCHEM

Solution structure of DnaE intein fromNostoc punctiforme: Structural basis for the design of a new split intein suitable for site-specific chemical modification

(2009) Jesper S. Oeemig et al.   FEBS LETTERS

The naturally splitNpuDnaE intein exhibits an extraordinarily high rate in the proteintrans-splicing reaction

(2009) Joachim Zettler et al.   FEBS LETTERS

Temperature-Sensitive Mutations Made Easy: Generating Conditional Mutations by Using Temperature-Sensitive Inteins That Function Within Different Temperature Ranges

(2009) G. Tan et al.   GENETICS

Novel Split Intein fortrans-Splicing Synthetic Peptide onto C Terminus of Protein

(2009) Julia H. Appleby et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Splicing of the Mycobacteriophage Bethlehem DnaB Intein

(2009) Kazuo Tori et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Selection and Structure of Hyperactive Inteins: Peripheral Changes Relayed to the Catalytic Center

(2009) Kaori Hiraga et al.   JOURNAL OF MOLECULAR BIOLOGY

Modeling Protein Splicing: Reaction Pathway for C-Terminal Splice and Intein Scission

(2009) Jon I. Mujika et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Protein Autoproteolysis: Conformational Strain Linked to the Rate of Peptide Cleavage by the pH Dependence of the N → O Acyl Shift Reaction

(2009) Denny G. A. Johansson et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Highly Conserved Histidine Plays a Dual Catalytic Role in Protein Splicing: A pKaShift Mechanism

(2009) Zhenming Du et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-Specific Two-Color Protein Labeling for FRET Studies Using Split Inteins

(2009) Jin-Yi Yang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Intein-mediated protein assembly in transgenic wheat: production of active barnase and acetolactate synthase from split genes

(2009) Katja Kempe et al.   PLANT BIOTECHNOLOGY JOURNAL

In Vivo and In Vitro Protein Ligation by Naturally Occurring and Engineered Split DnaE Inteins

(2009) A. Sesilja Aranko et al.   PLoS One

Protein C-Terminal Labeling and Biotinylation Using Synthetic Peptide and Split-Intein

(2009) Gerrit Volkmann et al.   PLoS One

Traceless protein splicing utilizing evolved split inteins

(2009) S. W. Lockless et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Modulation of intein activity by its neighboring extein substrates

(2009) G. Amitai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo

(2009) Anna Charalambous et al.   JOURNAL OF NANOBIOTECHNOLOGY

Activation of Protein Splicing by Protease- or Light-Triggered O to N Acyl Migration

(2008) Miquel Vila-Perelló et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-Specific Chemical Modification of Proteins with a Prelabelled Cysteine Tag Using the Artificially Split Mxe GyrA Intein

(2008) Thomas Kurpiers et al.   CHEMBIOCHEM

3-Mercaptopropionic acid-mediated synthesis of peptide and protein thioesters

(2008) Jaskiranjit Kang et al.   CHEMICAL COMMUNICATIONS

Interaction Studies and Alanine Scanning Analysis of a Semi-synthetic Split Intein Reveal Thiazoline Ring Formation from an Intermediate of the Protein Splicing Reaction

(2008) Christina Ludwig et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A novel hybrid seed system for plants

(2008) Mario Gils et al.   PLANT BIOTECHNOLOGY JOURNAL