Inteins: nature's gift to protein chemists

Oriented Covalent Immobilization of Antibodies for Measurement of Intermolecular Binding Forces between Zipper-Like Contact Surfaces of Split Inteins

(2013) Mirco Sorci et al.   ANALYTICAL CHEMISTRY

Structural basis for proteintrans-splicing by a bacterial intein-like domain - protein ligation without nucleophilic side chains

(2013) A. Sesilja Aranko et al.   FEBS Journal

Faster Protein Splicing with theNostoc punctiformeDnaE Intein Using Non-native Extein Residues

(2013) Manoj Cheriyan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Extein Residues Play an Intimate Role in the Rate-Limiting Step of Protein Trans-Splicing

(2013) Neel H. Shah et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Naturally Split Inteins Assemble through a “Capture and Collapse” Mechanism

(2013) Neel H. Shah et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Intermolecular domain swapping induces intein-mediated protein alternative splicing

(2013) A Sesilja Aranko et al.   Nature Chemical Biology

A conserved threonine spring-loads precursor for intein splicing

(2013) Albert K. Dearden et al.   PROTEIN SCIENCE

The Thermococcus kodakaraensis Tko CDC21-1 Intein Activates Its N-Terminal Splice Junction in the Absence of a Conserved Histidine by a Compensatory Mechanism

(2012) Kazuo Tori et al.   BIOCHEMISTRY

Mutual synergistic protein folding in split intein

(2012) Yuchuan Zheng et al.   BIOSCIENCE REPORTS

Recent progress in intein research: from mechanism to directed evolution and applications

(2012) Gerrit Volkmann et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Unprecedented Rates and Efficiencies Revealed for New Natural Split Inteins from Metagenomic Sources

(2012) Patricia Carvajal-Vallejos et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

NMR and Crystal Structures of the Pyrococcus horikoshii RadA Intein Guide a Strategy for Engineering a Highly Efficient and Promiscuous Intein

(2012) Jesper S. Oeemig et al.   JOURNAL OF MOLECULAR BIOLOGY

Streamlined Expressed Protein Ligation Using Split Inteins

(2012) Miquel Vila-Perelló et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

In-Cell Fluorescence Activation and Labeling of Proteins Mediated by FRET-Quenched Split Inteins

(2012) Radhika Borra et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ultrafast Protein Splicing is Common among Cyanobacterial Split Inteins: Implications for Protein Engineering

(2012) Neel H. Shah et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Photocontrol of Protein Activity Mediated by the Cleavage Reaction of a Split Intein

(2011) Jens Binschik et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Kinetic Control of One-Pot Trans-Splicing Reactions by Using a Wild-Type and Designed Split Intein

(2011) Neel H. Shah et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Branched Intermediate Formation Is the Slowest Step in the Protein Splicing Reaction of the Ala1 KlbA Intein fromMethanococcus jannaschii

(2011) Lana Saleh et al.   BIOCHEMISTRY

Highly Efficient and More Generalcis- andtrans-Splicing Inteins through Sequential Directed Evolution

(2011) Julia H. Appleby-Tagoe et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications

(2011) Brian P Callahan et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Evolution of cyclic peptide protease inhibitors

(2011) T. S. Young et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An overview of enzymatic reagents for the removal of affinity tags

(2011) David S. Waugh   PROTEIN EXPRESSION AND PURIFICATION

Biological Applications of Protein Splicing

(2010) Miquel Vila-Perelló et al.   CELL

Protein trans-splicing and its use in structural biology: opportunities and limitations

(2010) Gerrit Volkmann et al.   Molecular BioSystems

Branched intermediate formation stimulates peptide bond cleavage in protein splicing

(2010) Silvia Frutos et al.   Nature Chemical Biology

Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro

(2010) Mikko Muona et al.   Nature Protocols

Split Inteins as Versatile Tools for Protein Semisynthesis

(2009) Henning D. Mootz   CHEMBIOCHEM

Solution structure of DnaE intein fromNostoc punctiforme: Structural basis for the design of a new split intein suitable for site-specific chemical modification

(2009) Jesper S. Oeemig et al.   FEBS LETTERS

The naturally splitNpuDnaE intein exhibits an extraordinarily high rate in the proteintrans-splicing reaction

(2009) Joachim Zettler et al.   FEBS LETTERS

Novel Split Intein fortrans-Splicing Synthetic Peptide onto C Terminus of Protein

(2009) Julia H. Appleby et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Splicing of the Mycobacteriophage Bethlehem DnaB Intein

(2009) Kazuo Tori et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Selection and Structure of Hyperactive Inteins: Peripheral Changes Relayed to the Catalytic Center

(2009) Kaori Hiraga et al.   JOURNAL OF MOLECULAR BIOLOGY

Rapid selection of cyclic peptides that reduce α-synuclein toxicity in yeast and animal models

(2009) Joshua A Kritzer et al.   Nature Chemical Biology

Converging concepts of protein folding in vitro and in vivo

(2009) F Ulrich Hartl et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family

(2009) Bareket Dassa et al.   NUCLEIC ACIDS RESEARCH

In Vivo and In Vitro Protein Ligation by Naturally Occurring and Engineered Split DnaE Inteins

(2009) A. Sesilja Aranko et al.   PLoS One

Traceless protein splicing utilizing evolved split inteins

(2009) S. W. Lockless et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Modulation of intein activity by its neighboring extein substrates

(2009) G. Amitai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Inhibition of HIV Budding by a Genetically Selected Cyclic Peptide Targeting the Gag−TSG101 Interaction

(2008) Ali Tavassoli et al.   ACS Chemical Biology

Activation of Protein Splicing by Protease- or Light-Triggered O to N Acyl Migration

(2008) Miquel Vila-Perelló et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Protein Trans-Splicing as a Means for Viral Vector-Mediated In Vivo Gene Therapy

(2008) Juan Li et al.   HUMAN GENE THERAPY

Interaction Studies and Alanine Scanning Analysis of a Semi-synthetic Split Intein Reveal Thiazoline Ring Formation from an Intermediate of the Protein Splicing Reaction

(2008) Christina Ludwig et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Database resources of the National Center for Biotechnology Information

(2008) E. W. Sayers et al.   NUCLEIC ACIDS RESEARCH

Genetic Selection of Cyclic Peptide Dam Methyltransferase Inhibitors

(2007) Todd A. Naumann et al.   CHEMBIOCHEM