Iterative key-residues interrogation of a phytase with thermostability increasing substitutions identified in directed evolution

Multi-site saturation by OmniChange yields a pH- and thermally improved phytase

(2013) Amol V. Shivange et al.   JOURNAL OF BIOTECHNOLOGY

A multi-factors rational design strategy for enhancing the thermostability of Escherichia coli AppA phytase

(2013) Baojin Fei et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Mechanism of Protein Kinetic Stabilization by Engineered Disulfide Crosslinks

(2013) Inmaculada Sanchez-Romero et al.   PLoS One

Understanding Thermostability Factors of Aspergillus niger PhyA Phytase: A Molecular Dynamics Study

(2013) I. A. Noorbatcha et al.   PROTEIN JOURNAL

Directed evolution of a highly active Yersinia mollaretii phytase

(2011) Amol V. Shivange et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Initial Mutations Direct Alternative Pathways of Protein Evolution

(2011) Merijn L. M. Salverda et al.   PLoS Genetics

Phosphorothioate-based ligase-independent gene cloning (PLICing): An enzyme-free and sequence-independent cloning method

(2010) Milan Blanusa et al.   ANALYTICAL BIOCHEMISTRY

Conformational dynamics of active site loop in Escherichia coli phytase

(2010) Amol V. Shivange et al.   BIOPOLYMERS

Modifying Thermostability of appA from Escherichia coli

(2010) Weihua Zhu et al.   CURRENT MICROBIOLOGY

Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme

(2010) Kerstin Böhm et al.   FEBS Journal

Engineering of protease-resistant phytase from Penicillium sp.: High thermal stability, low optimal temperature and pH

(2010) Qianqian Zhao et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Semi-rational site-directed mutagenesis of phyI1s from Aspergillus niger 113 at two residue to improve its phytase activity

(2010) Yong-Sheng Tian et al.   MOLECULAR BIOLOGY REPORTS

Advances in generating functional diversity for directed protein evolution

(2009) Amol V Shivange et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Directed enzyme evolution: climbing fitness peaks one amino acid at a time

(2009) Cara A Tracewell et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Assembly of mutations for improving thermostability of Escherichia coli AppA2 phytase

(2008) Moon-Soo Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enhancing thermostability of Escherichia coli phytase AppA2 by error-prone PCR

(2008) Moon-Soo Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Phytate: impact on environment and human nutrition. A challenge for molecular breeding

(2008) Lisbeth Bohn et al.   Journal of Zhejiang University-SCIENCE B

Stabilization of Beta-Propeller Phytase by Introducing Xaa→Pro and Gly→Ala Substitutions at Consensus Positions

(2008) Kam-Bo Wong et al.   PROTEIN AND PEPTIDE LETTERS

Transversion-enriched sequence saturation mutagenesis (SeSaM-Tv+): A random mutagenesis method with consecutive nucleotide exchanges that complements the bias of error-prone PCR

(2007) Tuck Seng Wong et al.   Biotechnology Journal