Tackling HIV Persistence: Pharmacological versus CRISPR-Based Shock Strategies

Novel Latency Reversal Agents for HIV-1 Cure

(2018) Adam M. Spivak et al.   Annual Review of Medicine

Getting the “Kill” into “Shock and Kill”: Strategies to Eliminate Latent HIV

(2018) Youry Kim et al.   Cell Host & Microbe

HIV latency is reversed by ACSS2-driven histone crotonylation

(2018) Guochun Jiang et al.   JOURNAL OF CLINICAL INVESTIGATION

Latent HIV reservoirs exhibit inherent resistance to elimination by CD8+ T cells

(2018) Szu-Han Huang et al.   JOURNAL OF CLINICAL INVESTIGATION

Measuring replication competent HIV-1: advances and challenges in defining the latent reservoir

(2018) Zheng Wang et al.   Retrovirology

Inducible HIV RNA transcription assays to measure HIV persistence: pros and cons of a compromise

(2018) Johann Plantin et al.   Retrovirology

What do we measure when we measure cell-associated HIV RNA

(2018) Alexander O. Pasternak et al.   Retrovirology

Beyond the replication-competent HIV reservoir: transcription and translation-competent reservoirs

(2018) Amy E. Baxter et al.   Retrovirology

HIV latency in isolated patient CD4 + T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing

(2018) Steven A. Yukl et al.   Science Translational Medicine

CRISPR-Cas based antiviral strategies against HIV-1

(2018) Gang Wang et al.   VIRUS RESEARCH

Interval dosing with the HDAC inhibitor vorinostat effectively reverses HIV latency

(2017) Nancie M. Archin et al.   JOURNAL OF CLINICAL INVESTIGATION

In Vivo Excision of HIV-1 Provirus by saCas9 and Multiplex Single-Guide RNAs in Animal Models

(2017) Chaoran Yin et al.   MOLECULAR THERAPY

Novel AgoshRNA molecules for silencing of the CCR5 co-receptor for HIV-1 infection

(2017) Elena Herrera-Carrillo et al.   PLoS One

Innovations in the quantitative virus outgrowth assay and its use in clinical trials

(2017) Nicholas J. Norton et al.   Retrovirology

The mouse viral outgrowth assay: avatars for the detection of HIV-1 reservoirs

(2017) Kelly A. Metcalf Pate et al.   Retrovirology

HIV drug resistance against strand transfer integrase inhibitors

(2017) Kaitlin Anstett et al.   Retrovirology

HIV Latency: Should We Shock or Lock?

(2017) Gilles Darcis et al.   TRENDS IN IMMUNOLOGY

The Human Immunodeficiency Virus 1 ASP RNA promotes viral latency by recruiting the Polycomb Repressor Complex 2 and promoting nucleosome assembly

(2017) Juan C. Zapata et al.   VIROLOGY

Genome editing of the HIV co-receptors CCR5 and CXCR4 by CRISPR-Cas9 protects CD4+ T cells from HIV-1 infection

(2017) Zhepeng Liu et al.   Cell and Bioscience

HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study

(2017) Timothy J. Henrich et al.   PLOS MEDICINE

High-throughput Characterization of HIV-1 Reservoir Reactivation Using a Single-Cell-in-Droplet PCR Assay

(2017) Robert W. Yucha et al.   EBioMedicine

The effect of Ingenol-B on the suppressive capacity of elite suppressor HIV-specific CD8+ T cells

(2017) Abena K. Kwaa et al.   PLoS One

Exploring the anatomical HIV reservoirs

(2016) Gilles Darcis et al.   AIDS

Immune tolerance properties of the testicular tissue as a viral sanctuary site in ART-treated HIV-infected adults

(2016) Mohammad-Ali Jenabian et al.   AIDS

Bryostatin-1 for latent virus reactivation in HIV-infected patients on antiretroviral therapy

(2016) Carolina Gutiérrez et al.   AIDS

Attacking HIV-1 RNA versus DNA by sequence-specific approaches: RNAi versus CRISPR-Cas

(2016) Elena Herrera-Carrillo et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

HIV Reservoir: Finding the Right Needles in a Needlestack

(2016) Alexander O. Pasternak et al.   Cell Host & Microbe

The mTOR Complex Controls HIV Latency

(2016) Emilie Besnard et al.   Cell Host & Microbe

Total HIV-1 DNA, a Marker of Viral Reservoir Dynamics with Clinical Implications

(2016) Véronique Avettand-Fènoël et al.   CLINICAL MICROBIOLOGY REVIEWS

Excision of HIV-1 DNA by gene editing: a proof-of-concept in vivo study

(2016) R Kaminski et al.   GENE THERAPY

In Vivo Delivery Systems for Therapeutic Genome Editing

(2016) Luyao Wang et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Promising Role of Toll-Like Receptor 8 Agonist in Concert with Prostratin for Activation of Silent HIV

(2016) M. A. Rochat et al.   JOURNAL OF VIROLOGY

Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity

(2016) Josh Tycko et al.   MOLECULAR CELL

Potent and Targeted Activation of Latent HIV-1 Using the CRISPR/dCas9 Activator Complex

(2016) Sheena M Saayman et al.   MOLECULAR THERAPY

CRISPR-mediated Activation of Latent HIV-1 Expression

(2016) Prajit Limsirichai et al.   MOLECULAR THERAPY

CRISPR-Cas9 Can Inhibit HIV-1 Replication but NHEJ Repair Facilitates Virus Escape

(2016) Gang Wang et al.   MOLECULAR THERAPY

Specific Reactivation of Latent HIV-1 by dCas9-SunTag-VP64-mediated Guide RNA Targeting the HIV-1 Promoter

(2016) Haiyan Ji et al.   MOLECULAR THERAPY

PD-1+ and follicular helper T cells are responsible for persistent HIV-1 transcription in treated aviremic individuals

(2016) Riddhima Banga et al.   NATURE MEDICINE

A multifunctional AAV–CRISPR–Cas9 and its host response

(2016) Wei Leong Chew et al.   NATURE METHODS

Position effects influence HIV latency reversal

(2016) Heng-Chang Chen et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting

(2016) Jian Cao et al.   NUCLEIC ACIDS RESEARCH

CRISPR/Cas9: a double-edged sword when used to combat HIV infection

(2016) Chen Liang et al.   Retrovirology

Diversity of HIV-1 reservoirs in CD4+ T-cell subpopulations

(2016) Guinevere Q. Lee et al.   Current Opinion in HIV and AIDS

Preclinical shock strategies to reactivate latent HIV-1

(2016) Gilles Darcis et al.   Current Opinion in HIV and AIDS

Position effects influence HIV latency reversal

(2016) Heng-Chang Chen et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A Combinatorial CRISPR-Cas9 Attack on HIV-1 DNA Extinguishes All Infectious Provirus in Infected T Cell Cultures

(2016) Gang Wang et al.   Cell Reports

Development of 5‘ LTR DNA methylation of latent HIV-1 provirus in cell line models and in long-term-infected individuals

(2016) Kateřina Trejbalová et al.   Clinical Epigenetics

The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing

(2016) Christopher A. de Solis et al.   Frontiers in Molecular Neuroscience

HIV Latency-Reversing Agents Have Diverse Effects on Natural Killer Cell Function

(2016) Carolina Garrido et al.   Frontiers in Immunology

The Effect of Latency Reversal Agents on Primary CD8 + T Cells: Implications for Shock and Kill Strategies for Human Immunodeficiency Virus Eradication

(2016) Victoria E. Walker-Sperling et al.   EBioMedicine

Targeted HIV-1 Latency Reversal Using CRISPR/Cas9-Derived Transcriptional Activator Systems

(2016) Julia K. Bialek et al.   PLoS One

Elimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing

(2016) Rafal Kaminski et al.   Scientific Reports

Dendritic cell type-specific HIV-1 activation in effector T cells

(2015) Renée M. van der Sluis et al.   AIDS

HIV-1 Integration Landscape during Latent and Active Infection

(2015) Lillian B. Cohn et al.   CELL

Impact of the Timing of Initiation of Antiretroviral Therapy During Primary HIV-1 Infection on the Decay of Cell-Associated HIV-DNA

(2015) Moussa Laanani et al.   CLINICAL INFECTIOUS DISEASES

Sequential treatment with 5-aza-2'-deoxycytidine and deacetylase inhibitors reactivates HIV-1

(2015) S. Bouchat et al.   EMBO Molecular Medicine

The impact of HIV-1 genetic diversity on the efficacy of a combinatorial RNAi-based gene therapy

(2015) E Herrera-Carrillo et al.   GENE THERAPY

Ex vivo analysis identifies effective HIV-1 latency–reversing drug combinations

(2015) Gregory M. Laird et al.   JOURNAL OF CLINICAL INVESTIGATION

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

(2015) Silvana Konermann et al.   NATURE

Inducible in vivo genome editing with CRISPR-Cas9

(2015) Lukas E Dow et al.   NATURE BIOTECHNOLOGY

A split-Cas9 architecture for inducible genome editing and transcription modulation

(2015) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers

(2015) Isaac B Hilton et al.   NATURE BIOTECHNOLOGY

Highly efficient Cas9-mediated transcriptional programming

(2015) Alejandro Chavez et al.   NATURE METHODS

The CRISPR/Cas9 system inactivates latent HIV-1 proviral DNA

(2015) Weijun Zhu et al.   Retrovirology

Towards an HIV-1 cure: measuring the latent reservoir

(2015) Katherine M. Bruner et al.   TRENDS IN MICROBIOLOGY

CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust reactivation of the HIV-1 latent reservoirs

(2015) Yonggang Zhang et al.   Scientific Reports

An In-Depth Comparison of Latency-Reversing Agent Combinations in Various In Vitro and Ex Vivo HIV-1 Latency Models Identified Bryostatin-1+JQ1 and Ingenol-B+JQ1 to Potently Reactivate Viral Gene Expression

(2015) Gilles Darcis et al.   PLoS Pathogens

Synergistic Reactivation of Latent HIV Expression by Ingenol-3-Angelate, PEP005, Targeted NF-kB Signaling in Combination with JQ1 Induced p-TEFb Activation

(2015) Guochun Jiang et al.   PLoS Pathogens

The Depsipeptide Romidepsin Reverses HIV-1 Latency In Vivo

(2015) Ole S. Søgaard et al.   PLoS Pathogens

Short-term administration of disulfiram for reversal of latent HIV infection: a phase 2 dose-escalation study

(2015) Julian H Elliott et al.   Lancet HIV

Towards Antiviral shRNAs Based on the AgoshRNA Design

(2015) Ying Poi Liu et al.   PLoS One

A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging

(2014) Marvin E. Tanenbaum et al.   CELL

An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells

(2014) Federico González et al.   Cell Stem Cell

Development of HIV Reservoir Targeted Long Acting Nanoformulated Antiretroviral Therapies

(2014) Benson Edagwa et al.   CURRENT MEDICINAL CHEMISTRY

An HIV-Encoded Antisense Long Noncoding RNA Epigenetically Regulates Viral Transcription

(2014) Sheena Saayman et al.   MOLECULAR THERAPY

Rapid seeding of the viral reservoir prior to SIV viraemia in rhesus monkeys

(2014) James B. Whitney et al.   NATURE

HIV-1 persistence in CD4+ T cells with stem cell–like properties

(2014) Maria J Buzon et al.   NATURE MEDICINE

New ex vivo approaches distinguish effective and ineffective single agents for reversing HIV-1 latency in vivo

(2014) C Korin Bullen et al.   NATURE MEDICINE

Eradicating HIV-1 infection: seeking to clear a persistent pathogen

(2014) Nancie M. Archin et al.   NATURE REVIEWS MICROBIOLOGY

HIV-1 Latency in Monocytes/Macrophages

(2014) Amit Kumar et al.   Viruses-Basel

Activation of HIV Transcription with Short-Course Vorinostat in HIV-Infected Patients on Suppressive Antiretroviral Therapy

(2014) Julian H. Elliott et al.   PLoS Pathogens

Histone Deacetylase Inhibitors Impair the Elimination of HIV-Infected Cells by Cytotoxic T-Lymphocytes

(2014) Richard Brad Jones et al.   PLoS Pathogens

Panobinostat, a histone deacetylase inhibitor, for latent-virus reactivation in HIV-infected patients on suppressive antiretroviral therapy: a phase 1/2, single group, clinical trial

(2014) Thomas A Rasmussen et al.   Lancet HIV

Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system

(2013) Albert W Cheng et al.   CELL RESEARCH

A Pilot Study Assessing the Safety and Latency-Reversing Activity of Disulfiram in HIV-1-Infected Adults on Antiretroviral Therapy

(2013) A. M. Spivak et al.   CLINICAL INFECTIOUS DISEASES

HIV-associated chronic immune activation

(2013) Mirko Paiardini et al.   IMMUNOLOGICAL REVIEWS

The Distribution of HIV DNA and RNA in Cell Subsets Differs in Gut and Blood of HIV-Positive Patients on ART: Implications for Viral Persistence

(2013) Steven A. Yukl et al.   JOURNAL OF INFECTIOUS DISEASES

The Impact of Unprotected T Cells in RNAi-based Gene Therapy for HIV-AIDS

(2013) Elena Herrera-Carrillo et al.   MOLECULAR THERAPY

Marine natural products: Bryostatins in preclinical and clinical studies

(2013) Peter Kollár et al.   PHARMACEUTICAL BIOLOGY

Reactivation of latent HIV-1 in central memory CD4+ T cells through TLR-1/2 stimulation

(2013) Camille L Novis et al.   Retrovirology

HIV-1 transcription and latency: an update

(2013) Carine Van Lint et al.   Retrovirology

The lungs as anatomical reservoirs of HIV infection

(2013) Cecilia T. Costiniuk et al.   REVIEWS IN MEDICAL VIROLOGY

An In-Depth Comparison of Latent HIV-1 Reactivation in Multiple Cell Model Systems and Resting CD4+ T Cells from Aviremic Patients

(2013) Celsa A. Spina et al.   PLoS Pathogens

Post-Treatment HIV-1 Controllers with a Long-Term Virological Remission after the Interruption of Early Initiated Antiretroviral Therapy ANRS VISCONTI Study

(2013) Asier Sáez-Cirión et al.   PLoS Pathogens

Disulfiram reactivates latent HIV-1 expression through depletion of the phosphatase and tensin homolog

(2012) Geneviève Doyon et al.   AIDS

Stimulation of HIV-1-Specific Cytolytic T Lymphocytes Facilitates Elimination of Latent Viral Reservoir after Virus Reactivation

(2012) Liang Shan et al.   IMMUNITY

Cyclin T1 and CDK9 T-Loop Phosphorylation Are Downregulated during Establishment of HIV-1 Latency in Primary Resting Memory CD4+ T Cells

(2012) S. Budhiraja et al.   JOURNAL OF VIROLOGY

Administration of vorinostat disrupts HIV-1 latency in patients on antiretroviral therapy

(2012) N. M. Archin et al.   NATURE

HIV-1-encoded antisense RNA suppresses viral replication for a prolonged period

(2012) Mie Kobayashi-Ishihara et al.   Retrovirology

MicroRNA-Mediated Restriction of HIV-1 in Resting CD4+ T Cells and Monocytes

(2012) Karen Chiang et al.   Viruses-Basel

Epigenetic Silencing of HIV-1 by the Histone H3 Lysine 27 Methyltransferase Enhancer of Zeste 2

(2011) J. Friedman et al.   JOURNAL OF VIROLOGY

Regulation of Cyclin T1 and HIV-1 Replication by MicroRNAs in Resting CD4+ T Lymphocytes

(2011) K. Chiang et al.   JOURNAL OF VIROLOGY

A human memory T cell subset with stem cell–like properties

(2011) Luca Gattinoni et al.   NATURE MEDICINE

Discordance Between Cerebral Spinal Fluid and Plasma HIV Replication in Patients with Neurological Symptoms Who Are Receiving Suppressive Antiretroviral Therapy

(2010) Ana Canestri et al.   CLINICAL INFECTIOUS DISEASES

The Histone Deacetylase Inhibitor ITF2357 Decreases Surface CXCR4 and CCR5 Expression on CD4+ T-Cells and Monocytes and is Superior to Valproic Acid for Latent HIV-1 Expression in Vitro

(2010) Shay Matalon et al.   JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES

Involvement of Histone H3 Lysine 9 (H3K9) Methyltransferase G9a in the Maintenance of HIV-1 Latency and Its Reactivation by BIX01294

(2010) Kenichi Imai et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Complex and Evolving Story of T cell Activation to AAV Vector-encoded Transgene Products

(2010) Lauren E Mays et al.   MOLECULAR THERAPY

Molecular mechanisms of HIV-1 persistence in the monocyte-macrophage lineage

(2010) Valentin Le Douce et al.   Retrovirology

Molecular mechanisms of neuroinvasion by monocytes-macrophages in HIV-1 infection

(2010) Gabriel Gras et al.   Retrovirology

Expression of Latent HIV Induced by the Potent HDAC Inhibitor Suberoylanilide Hydroxamic Acid

(2009) Nancie M. Archin et al.   AIDS RESEARCH AND HUMAN RETROVIRUSES

Suberoylanilide Hydroxamic Acid Reactivates HIV from Latently Infected Cells

(2009) Xavier Contreras et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

HIV reservoir size and persistence are driven by T cell survival and homeostatic proliferation

(2009) Nicolas Chomont et al.   NATURE MEDICINE

Synergistic Activation of HIV-1 Expression by Deacetylase Inhibitors and Prostratin: Implications for Treatment of Latent Infection

(2009) Sophie Reuse et al.   PLoS One

Molecular control of HIV-1 postintegration latency: implications for the development of new therapeutic strategies

(2009) Laurence Colin et al.   Retrovirology

TLR5 stimulation is sufficient to trigger reactivation of latent HIV-1 provirus in T lymphoid cells and activate virus gene expression in central memory CD4+ T cells

(2009) Sandra Thibault et al.   VIROLOGY