Engineering bacteria for diagnostic and therapeutic applications

Rational Design of Evolutionarily Stable Microbial Kill Switches

(2017) Finn Stirling et al.   MOLECULAR CELL

Engineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammation

(2017) Kristina N‐M Daeffler et al.   Molecular Systems Biology

Complex cellular logic computation using ribocomputing devices

(2017) Alexander A. Green et al.   NATURE

Engineered bacteria can function in the mammalian gut long-term as live diagnostics of inflammation

(2017) David T Riglar et al.   NATURE BIOTECHNOLOGY

Engineering RGB color vision into Escherichia coli

(2017) Jesus Fernandez-Rodriguez et al.   Nature Chemical Biology

Large-scale recoding of a bacterial genome by iterative recombineering of synthetic DNA

(2017) Yu Heng Lau et al.   NUCLEIC ACIDS RESEARCH

Bacterial virulence phenotypes of Escherichia coli and host susceptibility determine risk for urinary tract infections

(2017) Henry L. Schreiber et al.   Science Translational Medicine

Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa gut infection in animal models

(2017) In Young Hwang et al.   Nature Communications

Antimicrobial Probiotics Reduce Salmonella enterica in Turkey Gastrointestinal Tracts

(2017) Brittany Forkus et al.   Scientific Reports

Reversal of Diabetes in NOD Mice by Clinical-Grade Proinsulin and IL-10–Secreting Lactococcus lactis in Combination With Low-Dose Anti-CD3 Depends on the Induction of Foxp3-Positive T Cells

(2016) Tatiana Takiishi et al.   DIABETES

Defining synonymous codon compression schemes by genome recoding

(2016) Kaihang Wang et al.   NATURE

Synchronous long-term oscillations in a synthetic gene circuit

(2016) Laurent Potvin-Trottier et al.   NATURE

Synchronized cycles of bacterial lysis for in vivo delivery

(2016) M. Omar Din et al.   NATURE

Tunable thermal bioswitches for in vivo control of microbial therapeutics

(2016) Dan I Piraner et al.   Nature Chemical Biology

Using promoter libraries to reduce metabolic burden due to plasmid-encoded proteins in recombinant Escherichia coli

(2016) Martina Pasini et al.   New Biotechnology

Design, synthesis, and testing toward a 57-codon genome

(2016) N. Ostrov et al.   SCIENCE

Synthetic recombinase-based state machines in living cells

(2016) N. Roquet et al.   SCIENCE

Disciplined approach to drug discovery and early development

(2016) Robert M. Plenge   Science Translational Medicine

A Minimal Model of Ribosome Allocation Dynamics Captures Trade-offs in Expression between Endogenous and Synthetic Genes

(2016) Thomas E. Gorochowski et al.   ACS Synthetic Biology

Oral Delivery of Pentameric Glucagon-Like Peptide-1 by Recombinant Lactobacillus in Diabetic Rats

(2016) Yin Lin et al.   PLoS One

Engineered Commensal Bacteria Reprogram Intestinal Cells Into Glucose-Responsive Insulin-Secreting Cells for the Treatment of Diabetes

(2015) Franklin F. Duan et al.   DIABETES

Genetically modified bacteria as a tool to detect microscopic solid tumor masses with triggered release of a recombinant biomarker

(2015) Jan T. Panteli et al.   Integrative Biology

Safety and Survival With GVAX Pancreas Prime and Listeria Monocytogenes–Expressing Mesothelin (CRS-207) Boost Vaccines for Metastatic Pancreatic Cancer

(2015) Dung T. Le et al.   JOURNAL OF CLINICAL ONCOLOGY

Recoded organisms engineered to depend on synthetic amino acids

(2015) Alexis J. Rovner et al.   NATURE

Biocontainment of genetically modified organisms by synthetic protein design

(2015) Daniel J. Mandell et al.   NATURE

'Deadman' and 'Passcode' microbial kill switches for bacterial containment

(2015) Clement T Y Chan et al.   Nature Chemical Biology

Quantifying cellular capacity identifies gene expression designs with reduced burden

(2015) Francesca Ceroni et al.   NATURE METHODS

Quorum-sensingSalmonellaselectively trigger protein expression within tumors

(2015) Charles A. Swofford et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates

(2015) Alexis Courbet et al.   Science Translational Medicine

Programmable probiotics for detection of cancer in urine

(2015) Tal Danino et al.   Science Translational Medicine

The outer mucus layer hosts a distinct intestinal microbial niche

(2015) Hai Li et al.   Nature Communications

Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota

(2015) Mark Mimee et al.   Cell Systems

Oral Delivery of Glutamic Acid Decarboxylase (GAD)-65 and IL10 by Lactococcus lactis Reverses Diabetes in Recent-Onset NOD Mice

(2014) S. Robert et al.   DIABETES

Incorporation of therapeutically modified bacteria into gut microbiota inhibits obesity

(2014) Zhongyi Chen et al.   JOURNAL OF CLINICAL INVESTIGATION

Mechanisms of immune tolerance to allergens: role of IL-10 and Tregs

(2014) Cezmi A. Akdis et al.   JOURNAL OF CLINICAL INVESTIGATION

A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase

(2014) T. H. Segall-Shapiro et al.   Molecular Systems Biology

A Transgenic Probiotic Secreting a Parasite Immunomodulator for Site-Directed Treatment of Gut Inflammation

(2014) Rose A Whelan et al.   MOLECULAR THERAPY

Rapid fucosylation of intestinal epithelium sustains host–commensal symbiosis in sickness

(2014) Joseph M. Pickard et al.   NATURE

Tunable protein degradation in bacteria

(2014) D Ewen Cameron et al.   NATURE BIOTECHNOLOGY

Permanent genetic memory with >1-byte capacity

(2014) Lei Yang et al.   NATURE METHODS

Oral Delivery of Glucagon Like Peptide-1 by a Recombinant Lactococcus lactis

(2014) Payal Agarwal et al.   PHARMACEUTICAL RESEARCH

Programmable bacteria detect and record an environmental signal in the mammalian gut

(2014) Jonathan W. Kotula et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Evolutionary meandering of intermolecular interactions along the drift barrier

(2014) Michael Lynch et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genomically encoded analog memory with precise in vivo DNA writing in living cell populations

(2014) F. Farzadfard et al.   SCIENCE

Modified Lactic Acid Bacteria Detect and Inhibit Multiresistant Enterococci

(2014) Juan Borrero et al.   ACS Synthetic Biology

Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras

(2014) David L. Shis et al.   ACS Synthetic Biology

Trial watch

(2014) Erika Vacchelli et al.   OncoImmunology

Phase 1b, multicenter, single blinded, placebo-controlled, sequential dose escalation study to assess the safety and tolerability of topically applied AG013 in subjects with locally advanced head and neck cancer receiving induction chemotherapy

(2013) Sewanti Atul Limaye et al.   CANCER

Oral Delivery of IL-27 Recombinant Bacteria Attenuates Immune Colitis in Mice

(2013) Miranda L. Hanson et al.   GASTROENTEROLOGY

Gut-on-a-Chip microenvironment induces human intestinal cells to undergo villus differentiation

(2013) Hyun Jung Kim et al.   Integrative Biology

Precise and reliable gene expression via standard transcription and translation initiation elements

(2013) Vivek K Mutalik et al.   NATURE METHODS

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

Genetically Programmable Pathogen Sense and Destroy

(2013) Saurabh Gupta et al.   ACS Synthetic Biology

Reprogramming Microbes to Be Pathogen-Seeking Killers

(2013) In Young Hwang et al.   ACS Synthetic Biology

Visualization of Evolutionary Stability Dynamics and Competitive Fitness of Escherichia coli Engineered with Randomized Multigene Circuits

(2013) Sean C. Sleight et al.   ACS Synthetic Biology

Reversal of autoimmune diabetes by restoration of antigen-specific tolerance using genetically modified Lactococcus lactis in mice

(2012) Tatiana Takiishi et al.   JOURNAL OF CLINICAL INVESTIGATION

Rewritable digital data storage in live cells via engineered control of recombination directionality

(2012) J. Bonnet et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Food-Grade Bacteria Expressing Elafin Protect Against Inflammation and Restore Colon Homeostasis

(2012) J.-P. Motta et al.   Science Translational Medicine

In Vivo Gene Expression Dynamics of Tumor-Targeted Bacteria

(2012) Tal Danino et al.   ACS Synthetic Biology

A Live-Attenuated Listeria Vaccine (ANZ-100) and a Live-Attenuated Listeria Vaccine Expressing Mesothelin (CRS-207) for Advanced Cancers: Phase I Studies of Safety and Immune Induction

(2011) D. T. Le et al.   CLINICAL CANCER RESEARCH

Attenuated Listeria monocytogenes vaccine vectors expressing Influenza A nucleoprotein: preclinical evaluation and oral inoculation of volunteers

(2011) Paul V. Johnson et al.   MICROBIOLOGY AND IMMUNOLOGY

Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen

(2011) N. Saeidi et al.   Molecular Systems Biology

Prevention of vaginal SHIV transmission in macaques by a live recombinant Lactobacillus

(2011) L A Lagenaur et al.   Mucosal Immunology

Essential information for synthetic DNA sequences

(2011) Jean Peccoud et al.   NATURE BIOTECHNOLOGY

antiSMASH: rapid identification, annotation and analysis of secondary metabolite biosynthesis gene clusters in bacterial and fungal genome sequences

(2011) Marnix H. Medema et al.   NUCLEIC ACIDS RESEARCH

GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules

(2011) Alejandro Sarrion-Perdigones et al.   PLoS One

Extensive personal human gut microbiota culture collections characterized and manipulated in gnotobiotic mice

(2011) A. L. Goodman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An intranasal administration of Lactococcus lactis strains expressing recombinant interleukin-10 modulates acute allergic airway inflammation in a murine model

(2010) F. A. V. Marinho et al.   CLINICAL AND EXPERIMENTAL ALLERGY

Vaccination Strategies to Promote Mucosal Antibody Responses

(2010) Kang Chen et al.   IMMUNITY

Treatment of colitis with a commensal gut bacterium engineered to secrete human tgf-β1 under the control of dietary xylan

(2010) Zaed Z.R. Hamady et al.   INFLAMMATORY BOWEL DISEASES

Gut inflammation provides a respiratory electron acceptor for Salmonella

(2010) Sebastian E. Winter et al.   NATURE

AG013, a mouth rinse formulation of Lactococcus lactis secreting human Trefoil Factor 1, provides a safe and efficacious therapeutic tool for treating oral mucositis

(2010) Silvia Caluwaerts et al.   ORAL ONCOLOGY

Engineered bacterial communication prevents Vibrio cholerae virulence in an infant mouse model

(2010) F. Duan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Synthetic Genetic Edge Detection Program

(2009) Jeffrey J. Tabor et al.   CELL

Bacterial delivery of a novel cytolysin to hypoxic areas of solid tumors

(2009) R M Ryan et al.   GENE THERAPY

Orally administered L. lactis secreting an anti-TNF Nanobody demonstrate efficacy in chronic colitis

(2009) K Vandenbroucke et al.   Mucosal Immunology

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Synthetic Gene Networks That Count

(2009) A. E. Friedland et al.   SCIENCE

The first clinical use of a live-attenuated Listeria monocytogenes vaccine: A Phase I safety study of Lm-LLO-E7 in patients with advanced carcinoma of the cervix

(2009) Paulo Cesar Maciag et al.   VACCINE

Construction and Characterization of an Attenuated Listeria monocytogenes Strain for Clinical Use in Cancer Immunotherapy

(2008) A. Wallecha et al.   Clinical and Vaccine Immunology

Discovering and improving novel peptide therapeutics

(2008) D MCGREGOR   CURRENT OPINION IN PHARMACOLOGY

A fast, robust and tunable synthetic gene oscillator

(2008) Jesse Stricker et al.   NATURE

Paracellular entry of interleukin-10 producing Lactococcus lactis in inflamed intestinal mucosa in mice

(2007) Anouk Waeytens et al.   INFLAMMATORY BOWEL DISEASES