Article
Multidisciplinary Sciences
Albert W. Hinman, Hsin-Yi Yeh, Baptiste Roelens, Kei Yamaya, Alexander Woglar, Henri-Marc G. Bourbon, Peter Chi, Anne M. Villeneuve
Summary: Meiotic recombination plays essential roles in genetic diversity and genome inheritance, with DSB-3 identified as a protein promoting DSB formation in the nematode Caenorhabditis elegans. DSB-3 is interdependent with other DSB proteins and its localization supports its role as a homolog of MEI4 in conserved DSB-promoting complexes. Variations in meiotic programs across diverse organisms may contribute to the diversification of essential meiotic machinery components.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Hamida Laroussi, Ariadna B. Juarez-Martinez, Aline Le Roy, Elisabetta Boeri Erba, Frank Gabel, Bernard de Massy, Jan Kadlec
Summary: Meiotic recombination in mice is initiated by DNA double-strand breaks (DSBs), catalyzed by the TOPOVIL complex. This complex's activity is regulated by meiotic factors such as REC114, MEI4, and IHO1, but the mechanism is not well understood. This study reveals the molecular details of these factors and their interactions, including REC114 forming homodimers, associating with MEI4 as a heterotrimer, and IHO1 forming tetramers. The study also shows that IHO1 directly interacts with REC114 and shares a binding surface with TOPOVIBL and another meiotic factor ANKRD31. These findings suggest the existence of a ternary IHO1-REC114-MEI4 complex and highlight the potential regulatory role of REC114 in mediating interactions with multiple partners.
Article
Multidisciplinary Sciences
Dominic Johnson, Margaret Crawford, Tim Cooper, Corentin Claeys Bouuaert, Scott Keeney, Bertrand Llorente, Valerie Garcia, Matthew J. Neale
Summary: Genetic recombination during meiosis involves the repair of DNA double-strand breaks (DSBs) created by Spo11, with recent findings indicating the existence of multiple Spo11 DSBs termed 'double cuts'. These double cuts vary in length with a periodicity of 10.5 base pairs and play a role in recombination and DNA gap repair during meiosis.
Article
Biochemistry & Molecular Biology
Rachel Bayley, Valerie Borel, Rhiannon J. Moss, Ellie Sweatman, Philip Ruis, Alice Ormrod, Amalia Goula, Rachel M. A. Mottram, Tyler Stanage, Graeme Hewitt, Marco Saponaro, Grant S. Stewart, Simon J. Boulton, Martin R. Higgs
Summary: The study suggests that methylation of histone H3 by SETD1A-BOD1L contributes to the recruitment of RIF1 to double-strand breaks, preventing excessive degradation of DNA ends and maintaining genome stability. This mechanism plays a crucial role in preserving telomere function and class switch recombination, and may be associated with the treatment and resistance of certain cancers.
Article
Cell Biology
Kaixian Liu, Emily M. Grasso, Stephen Pu, Mengyang Zou, Shixin Liu, David Eliezer, Scott Keeney
Summary: This study reports the structure and dynamic DNA binding properties of the Rec114 and Mei4 protein complex, which initiates DNA double-strand breaks during meiotic recombination. The complex can bridge multiple DNA duplexes and generate force to condense DNA through long-range interactions. These findings provide insight into the conserved networks of protein-protein and protein-DNA interactions that promote condensate formation and meiotic DSBs.
GENES & DEVELOPMENT
(2023)
Article
Biology
Heyun Guo, Ericca L. Stamper, Aya Sato-Carlton, Masa A. Shimazoe, Xuan Li, Liangyu Zhang, Lewis Stevens, K. C. Jacky Tam, Abby F. Dernburg, Peter M. Carlton
Summary: In the nematode Caenorhabditis elegans, the level of double-strand breaks (DSBs) during meiotic cell division is regulated by the phosphorylation of DSB-1. The phosphorylation of DSB-1 is controlled by the opposing activities of the PP4 phosphatase and the ATR kinase. The loss of DSB-2, a paralog of DSB-1, leads to a decrease in DSB formation. The results suggest that DSB-1 phosphorylation is compensated by DSB-2 in order to maintain optimal levels of DSBs in older animals.
Article
Biochemistry & Molecular Biology
Sijie Liu, Yu Hua, Jingna Wang, Lingyan Li, Junjie Yuan, Bo Zhang, Ziyang Wang, Jianguo Ji, Daochun Kong
Summary: Protection of 30 overhangs in DNA double-strand breaks (DSBs) repair is achieved through the transient formation of RNA-DNA hybrids, with RNA polymerase III (RNAPIII) responsible for synthesizing the RNA strand. CtIP and MRN nuclease activity are required for initiating RNAPIII-mediated RNA synthesis at DSBs. Reduced RNAPIII levels suppress homologous recombination (HR) and lead to genetic loss > 30 bp at DSBs.
Review
Cell Biology
Corinne Grey, Bernard de Massy
Summary: The axial element plays a crucial role in establishing sister chromatid cohesion and meiotic recombination during prophase I of meiosis, contributing to the successful outcome of meiosis I.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Vikash Kumar Yadav, Corentin Claeys Bouuaert
Summary: The article outlines the latest advances in understanding the mechanism of meiotic DSB formation, emphasizing the tight regulation of DSBs and the significance of recombination mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Catriona Munro, Hugo Cadis, Sophie Pagnotta, Evelyn Houliston, Jean-Rene Huynh
Summary: During meiosis, DNA recombination mediated by Spo11 enzyme triggers double-strand breaks (DSBs), which lead to the shuffling of genetic information between maternal and paternal chromosomes. In a study on the jellyfish Clytia hemisphaerica, they found that knocking down the Spo11 gene resulted in the failure of germ cells to assemble synaptonemal complexes and chiasmata, leading to the presence of unassociated homologous chromosome pairs and aneuploid but fertilizable eggs. This study provides significant evolutionary perspectives on meiosis regulation, as Clytia belongs to the sister clade of classical animal model species.
Article
Biophysics
Neha Chauhan, Apostolos Karanastasis, Chaitanya K. Ullal, Xing Wang
Summary: Homologous pairing (HP) is a fundamental biological process that is not fully understood. This study reveals that HP can occur in short DNA sequences as small as 18 basepairs. By analyzing crystal formation in a colloidal micro-particle-based system, the impact of nucleotide order and triplet framework on HP is validated.
BIOPHYSICAL JOURNAL
(2022)
Review
Cell Biology
Marcelo Santos da Silva
Summary: This article discusses the unique dependence of trypanosomatids on DSBs for important events such as antigenic variation, genetic exchange, and genomic changes, as well as how they balance the benefits and challenges caused by DSBs through homologous recombination.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Genetics & Heredity
Liat Morciano, Renana M. Elgrabli, Drora Zenvirth, Ayelet Arbel-Eden
Summary: Several events occur during meiosis to reshape the genome and transfer it to the next generation. The occurrence of new meiotic mutations is closely linked to homologous recombination and depends on Spo11-induced DNA breaks. Through studying the timing of mutation and recombination events in cells deficient in DNA HR-repair genes, we found that Rad54 and Tid1 play different roles in meiotic mutation occurrence. We also observed that single-stranded DNA may be a potential source for mutagenicity during meiosis. Overall, we propose that de novo mutations during meiosis contribute to the diversification of the genome.
Article
Multidisciplinary Sciences
Silvia Prieler, Doris Chen, Lingzhi Huang, Elisa Mayrhofer, Soma Zsoter, Magdalena Vesely, Jean Mbogning, Franz Klein
Summary: Meiotic recombination is initiated by programmed DNA double-strand breaks introduced by Spo11, generating previously uncharacterized double DSB gaps of varying lengths. These gaps exhibit a periodic length pattern and are enriched at DSB hotspots, indicating a preference for sequences with DNA-bending motifs. Double DSB signals overlap with topoisomerase II-binding sites, suggesting a role for topological stress and DNA crossings in break formation. This process can lead to evolutionary diversity and pathogenic germline aberrations.
Article
Plant Sciences
Yan Wang, Shu-Yue Li, Ya-Zhong Wang, Yan He
Summary: By integrating genetic analysis, immunostaining technology, and protein-protein interaction studies, the putative factors linking DSB formation to chromosome axis were explored in maize meiosis. It was found that ZmASY1 and ZmPRD3 may work as a key module linking DSB sites to chromosome axes during DSB formation in maize.
Article
Biotechnology & Applied Microbiology
Luis A. Vale-Silva, Tovah E. Markowitz, Andreas Hochwagen
Article
Cell Biology
Jagadeeswara Rao Bommi, Hanumanthu Bala Durga Prasada Rao, Kiran Challa, Mika Higashide, Kaori Shinmyozu, Jun-ichi Nakayama, Miki Shinohara, Akira Shinohara
Article
Genetics & Heredity
Kiran Challa, Ghanim Fajish, Miki Shinohara, Franz Klein, Susan M. Gasser, Akira Shinohara
Review
Genetics & Heredity
Kiran Challa, Miki Shinohara, Akira Shinohara
Article
Multidisciplinary Sciences
Vijayalakshmi V. Subramanian, Xuan Zhu, Tovah E. Markowitz, Luis A. Vale-Silva, Pedro A. San-Segundo, Nancy M. Hollingsworth, Scott Keeney, Andreas Hochwagen
NATURE COMMUNICATIONS
(2019)
Article
Genetics & Heredity
Miki Shinohara, Douglas K. Bishop, Akira Shinohara
Article
Multidisciplinary Sciences
Shinji Hirotsune, Hiroshi Kiyonari, Mingyue Jin, Kanako Kumamoto, Kayo Yoshida, Miki Shinohara, Hitomi Watanabe, Anthony Wynshaw-Boris, Fumio Matsuzaki
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Ying Zhang, Takuya Suzuki, Ke Li, Santosh K. Gothwal, Miki Shinohara, Akira Shinohara
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biology
Zhihui Zhu, Mohammad Bani Ismail, Miki Shinohara, Akira Shinohara
Summary: Depletion of Cdc53 leads to defects in synaptonemal complex formation during meiosis while maintaining crossover formation, suggesting the uncoupling of meiotic recombination with synaptonemal complex assembly. The collaboration between Pch2 and Cdc53 is critical for synaptonemal complex assembly and meiotic recombination.
LIFE SCIENCE ALLIANCE
(2021)
Article
Biochemistry & Molecular Biology
Min-Su Lee, Mika T. Higashide, Hyungseok Choi, Ke Li, Soogil Hong, Kangseok Lee, Akira Shinohara, Miki Shinohara, Keun P. Kim
Summary: The Ecm11-Gmc2 (EG) complex plays a critical role in meiotic recombination in yeast by regulating double-strand break formation and crossover frequencies, while the SC central region is essential for controlling specificity during meiosis recombination.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Genetics & Heredity
Krishnaprasad G. Nandanan, Sagar Salim, Ajith Pankajam, Miki Shinohara, Gen Lin, Parijat Chakraborty, Amamah Farnaz, Lars M. Steinmetz, Akira Shinohara, Koodali T. Nishant
Summary: The Msh4-Msh5 complex in baker's yeast plays a vital role in meiotic crossovers and is found to associate specifically with DSB hotspots, chromosome axes, and centromeres. Its binding is enhanced by DSB formation and resection, and is correlated to DSB frequency, as well as being influenced by chromosome size and density of crossovers. These findings shed light on how Msh4-Msh5 works with other pro-crossover factors to ensure crossover formation.
Article
Biology
Hanumanthu B. D. Prasada Rao, Takeshi Sato, Kiran Challa, Yurika Fujita, Miki Shinohara, Akira Shinohara
Summary: During meiosis, CDK and DDK regulate the dynamics of Mps3 on the nuclear envelope by mediating the formation of Mps3 clusters and telomere clustering. The luminal region of Mps3 plays a crucial role in the meiosis-specific localisation of Mps3 on the nuclear envelope. Phosphorylation-dependent regulation of Mps3 localisation on the nuclear envelope is essential for meiotic chromosome motion and nuclear envelope remodelling.
Article
Multidisciplinary Sciences
Kenichiro Matsuzaki, Kenji Kumatoriya, Mizuki Tando, Takashi Kometani, Miki Shinohara
Summary: Acetaldehyde, a metabolic product of ethanol, induces DNA damage and genome instability. The repair of acetaldehyde-induced DNA damage relies on both non-homologous end-joining (NHEJ) and homology-directed repair (HDR). Additionally, polyphenols extracted from persimmon fruits can attenuate acetaldehyde-induced DNA damage.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Miki Shinohara, Akira Shinohara
Summary: Meiotic crossing over is essential for the segregation of homologous chromosomes. The formation and distribution of meiotic crossovers (COs) are tightly regulated. This study analyzed the localization of proteins involved in meiotic recombination in xrs2 hypomorphic mutants and found a proportional relationship between the number of cytological foci with recombinases and the DSB numbers. The study also revealed a chromosome-specific homeostatic response of COs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)