Article
Plant Sciences
Yan Wang, Shu-Yue Li, Ya-Zhong Wang, Yan He
Summary: By integrating genetic analysis, immunostaining technology, and protein-protein interaction studies, the putative factors linking DSB formation to chromosome axis were explored in maize meiosis. It was found that ZmASY1 and ZmPRD3 may work as a key module linking DSB sites to chromosome axes during DSB formation in maize.
Article
Cell Biology
Takehiko Usui, Akira Shinohara
Summary: This study reveals that meiotic cells activate Rad53 in response to exogenous DSBs, dependent on histone H3K79 methylation, but Rad9 is insensitive to meiotic programmed DSBs due to its inability to bind to DSBs. Artifical tethering of Rad9 to meiotic DSBs activates Rad53, leading to decreased repair of meiotic DSBs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Albert W. Hinman, Hsin-Yi Yeh, Baptiste Roelens, Kei Yamaya, Alexander Woglar, Henri-Marc G. Bourbon, Peter Chi, Anne M. Villeneuve
Summary: Meiotic recombination plays essential roles in genetic diversity and genome inheritance, with DSB-3 identified as a protein promoting DSB formation in the nematode Caenorhabditis elegans. DSB-3 is interdependent with other DSB proteins and its localization supports its role as a homolog of MEI4 in conserved DSB-promoting complexes. Variations in meiotic programs across diverse organisms may contribute to the diversification of essential meiotic machinery components.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Yazhong Wang, Yan Wang, Jie Zang, Huabang Chen, Yan He
Summary: The function of PRD1 in regulating meiotic DSB formation is conserved in maize, but its role in bipolar spindle assembly shows divergence among different plant species. ZmPRD1 is essential for DSB formation and homologous recombination in maize meiosis, but the function of PRD1 in bipolar spindle assembly during rice meiosis is not conserved in maize.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Multidisciplinary Sciences
Alexandre Nore, Ariadna B. Juarez-Martinez, Julie Clement, Christine Brun, Boubou Diagouraga, Hamida Laroussi, Corinne Grey, Henri Marc Bourbon, Jan Kadlec, Thomas Robert, Bernard de Massy
Summary: This study identifies REC114 as a direct partner of TOPOVIBL and demonstrates that their interaction is crucial for efficient and timely DNA double-strand break (DSB) activity during meiosis.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Hamida Laroussi, Ariadna B. Juarez-Martinez, Aline Le Roy, Elisabetta Boeri Erba, Frank Gabel, Bernard de Massy, Jan Kadlec
Summary: Meiotic recombination in mice is initiated by DNA double-strand breaks (DSBs), catalyzed by the TOPOVIL complex. This complex's activity is regulated by meiotic factors such as REC114, MEI4, and IHO1, but the mechanism is not well understood. This study reveals the molecular details of these factors and their interactions, including REC114 forming homodimers, associating with MEI4 as a heterotrimer, and IHO1 forming tetramers. The study also shows that IHO1 directly interacts with REC114 and shares a binding surface with TOPOVIBL and another meiotic factor ANKRD31. These findings suggest the existence of a ternary IHO1-REC114-MEI4 complex and highlight the potential regulatory role of REC114 in mediating interactions with multiple partners.
Article
Biochemistry & Molecular Biology
Cunliang Li, Yuyu Guo, Lili Wang, Shunping Yan
Summary: DNA double-strand breaks (DSBs) are highly toxic forms of DNA damage that pose a threat to genome stability. Homologous recombination is an error-free pathway for DSB repair, in which the evolutionarily conserved SMC5/6 complex plays crucial roles. It has been discovered that SMC5/6 recruits the PAF1 complex (PAF1C) to facilitate DSB repair in plants.
Article
Multidisciplinary Sciences
Catriona Munro, Hugo Cadis, Sophie Pagnotta, Evelyn Houliston, Jean-Rene Huynh
Summary: During meiosis, DNA recombination mediated by Spo11 enzyme triggers double-strand breaks (DSBs), which lead to the shuffling of genetic information between maternal and paternal chromosomes. In a study on the jellyfish Clytia hemisphaerica, they found that knocking down the Spo11 gene resulted in the failure of germ cells to assemble synaptonemal complexes and chiasmata, leading to the presence of unassociated homologous chromosome pairs and aneuploid but fertilizable eggs. This study provides significant evolutionary perspectives on meiosis regulation, as Clytia belongs to the sister clade of classical animal model species.
Article
Biochemistry & Molecular Biology
Teresa Giannattasio, Erika Testa, Monica Faieta, Matteo Lampitto, Daniela Nardozi, Stefano di Cecca, Antonella Russo, Marco Barchi
Summary: XY chromosome missegregation is common in humans and can lead to sterility or the generation of aneuploid spermatozoa. This study investigates the lack of double-strand breaks (DSBs) in the pseudoautosomal region (PAR) as a leading cause of XY missegregation in mice. The study demonstrates that the genetic background of mice affects the length of chromatin loops in the PAR and the proficiency of XY recombination. The findings suggest that varying expression of Spo11 splice isoforms can recover XY recombination and synapsis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Multidisciplinary Sciences
Silvia Prieler, Doris Chen, Lingzhi Huang, Elisa Mayrhofer, Soma Zsoter, Magdalena Vesely, Jean Mbogning, Franz Klein
Summary: Meiotic recombination is initiated by programmed DNA double-strand breaks introduced by Spo11, generating previously uncharacterized double DSB gaps of varying lengths. These gaps exhibit a periodic length pattern and are enriched at DSB hotspots, indicating a preference for sequences with DNA-bending motifs. Double DSB signals overlap with topoisomerase II-binding sites, suggesting a role for topological stress and DNA crossings in break formation. This process can lead to evolutionary diversity and pathogenic germline aberrations.
Article
Biochemistry & Molecular Biology
Alexander M. Weitzel, Hannah M. Grunwald, Ceri Weber, Rimma L. Levina, Valentino Gantz, Stephen Hedrick, Ethan Bier, Kimberly Cooper
Summary: Efficient gene conversion systems using CRISPR/Cas9 have the potential to study genetic traits in mice and limit loss of biodiversity caused by wild rodents. The timing of Cas9 expression during meiosis I is critical for successful gene conversion, though current low expression levels may hinder efficient DSB formation. Increasing Cas9 expression levels during early meiosis I could improve the efficiency of gene conversion in both male and female mice.
Review
Genetics & Heredity
Judith Mine-Hattab, Siyu Liu, Angela Taddei
Summary: This article discusses the physical nature of DNA repair foci, including liquid-liquid phase separation and its role in the formation and disassembly of foci. The article introduces different models of condensates and discusses evidence of liquid phase separation at DNA repair sites, and finally explores the origin and possible function of liquid phase separation in DNA repair processes.
Article
Biochemistry & Molecular Biology
Sahar Waseem, Sudeep Kumar, Kanghoon Lee, Byoung-Ha Yoon, Mirang Kim, Hail Kim, Keesook Lee
Summary: PRMT1 is crucial for male fertility in mice, as its deficiency leads to impaired repair pathway of DNA double-strand breaks in male germ cells. This study provides new insight into the role of PRMT1-mediated asymmetric demethylation in mouse spermatogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Thorben Sprink, Frank Hartung
Summary: This study investigates the specific function and evolution of two meiotic SPO11 paralogs in land plants and reveals their sequence-specific function between species, providing important clues for understanding meiosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Stephanie M. Ackerson, Carlan Romney, P. Logan Schuck, Jason A. Stewart
Summary: Regulation of DNA double-strand breaks and telomeres in cells is diametrically opposed, with DSBs requiring quick recognition and repair while telomeres must be protected to prevent unwanted chromosome fusions. Decision on whether to join DNA ends is critical for genome stability, and processing of telomeres and DSBs share commonalities. Repair of DSBs is determined by decision points that shift towards homologous recombination (HR) or non-homologous end joining (NHEJ).
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Entomology
Garima Dixit, Ashish Praveen, Tusha Tripathi, Vikash Kumar Yadav, Praveen C. Verma
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Vrijesh Kumar Yadav, Vikash Kumar Yadav, Poonam Pant, Surendra Pratap Singh, Rashmi Maurya, Anshulika Sable, Samir V. Sawant
PLANT BIOTECHNOLOGY JOURNAL
(2017)
Article
Entomology
Garima Dixit, Ashish Praveen, Tusha Tripathi, Vikash Kumar Yadav, Praveen C. Verma
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
(2017)
Article
Plant Sciences
Chandrawati, Neha Singh, Rajendra Kumar, Sujit Kumar, P. K. Singh, V. K. Yadav, S. A. Ranade, Hemant Kumar Yadav
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2017)
Article
Biochemistry & Molecular Biology
Vikash Kumar Yadav, Juan Santos-Gonzalez, Claudia Kohler
Summary: The study established a protocol combining INTACT and Hi-C for studying limited nuclei in plants, revealing distinct higher-order chromatin structures in different cell types and the close correlation between chromatin interactions and transcriptional activity.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Genetics & Heredity
Vikash Kumar Yadav, Swadha Singh, Amrita Yadav, Neha Agarwal, Babita Singh, Siddhi Kashinath Jalmi, Vrijesh Kumar Yadav, Vipin Kumar Tiwari, Verandra Kumar, Raghvendra Singh, Samir Vishwanath Sawant
Summary: This study assessed the impact of stress conditions on the chromatin-interactome network in Arabidopsis thaliana. The results showed that stress conditions could modulate the dynamics of chromatin interactions. It was also observed that interactions were more common among chromatin loci with the same epigenetic state, suggesting a clustering effect. These findings highlight the importance of chromatin interactions in response to stress and their role in chromatin folding.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Vikash Kumar Yadav, Samir Vishwanath Sawant, Amrita Yadav, Siddhi Kashinath Jalmi, Savita Kerkar
Summary: In this study, the researchers investigated the role of long non-coding RNAs (lncRNAs) in regulating the circadian clock in Arabidopsis thaliana. They found that the expression of lncRNAs varied under different light conditions and were associated with light-responsive genes, flower development, and the circadian clock. The study also revealed interactions between lncRNAs and miRNAs, which regulated genes involved in development, gene silencing, and transcription regulation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Multidisciplinary Sciences
Vikash Kumar Yadav, Siddhi Kashinath Jalmi, Shalini Tiwari, Savita Kerkar
Summary: This study investigated the characteristics and features of lncRNAs in Arabidopsis thaliana, Oryza sativa, and Zea mays. The results showed that lncRNAs were distributed throughout the chromosomes, enriched in the Helitron family of transposable elements, and depleted in regions with terminal inverted repeats. Additionally, lncRNA transcribing regions had rare or weak signals for most epigenetic marks except for H3K9me2 and cytosine methylation. The study also found conserved endogenous target sites in the lncRNAs and identified unique miRNAs potentially targeting the lncRNAs in each plant species. The miRNA-targeted lncRNAs were found to interact with transcription factors and play a role in diverse biological and molecular processes.
SCIENTIFIC REPORTS
(2023)
