Article
Biochemistry & Molecular Biology
Cunliang Li, Yuyu Guo, Lili Wang, Shunping Yan
Summary: DNA double-strand breaks (DSBs) are highly toxic forms of DNA damage that pose a threat to genome stability. Homologous recombination is an error-free pathway for DSB repair, in which the evolutionarily conserved SMC5/6 complex plays crucial roles. It has been discovered that SMC5/6 recruits the PAF1 complex (PAF1C) to facilitate DSB repair in plants.
Review
Biochemistry & Molecular Biology
Chiara Frigerio, Elena Di Nisio, Michela Galli, Chiara Vittoria Colombo, Rodolfo Negri, Michela Clerici
Summary: DNA double-strand breaks (DSBs) are harmful DNA lesions that can lead to catastrophic consequences for genome stability. DSBs can be repaired by non-homologous end joining (NHEJ) or homologous recombination (HR), depending on the proteins that bind to the DSB ends and their regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Vikash Kumar Yadav, Corentin Claeys Bouuaert
Summary: The article outlines the latest advances in understanding the mechanism of meiotic DSB formation, emphasizing the tight regulation of DSBs and the significance of recombination mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shuai Zhao, Kelly N. Chuh, Baichao Zhang, Barbara E. Dul, Robert E. Thompson, Lorna A. Farrelly, Xiaohui Liu, Ning Xu, Yi Xue, Robert G. Roeder, Ian Maze, Tom W. Muir, Haitao Li
Summary: The study found that the readers of H3K4me3 retain binding to H3K4me3Q5ser, while the erasers are significantly inhibited. The results suggest that H3Q5ser may potentially fine-tune critical gene expression programs by stabilizing H3K4me3 from dynamic turnover or enhancing its physical readout by downstream effectors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Kortany M. Baker, Smriti Hoda, Debasmita Saha, Justin B. Gregor, Livia Georgescu, Nina D. Serratore, Yueping Zhang, Lizhi Cheng, Nadia A. Lanman, Scott D. Briggs
Summary: This study reveals that Set1 mediates histone H3K4 methylation in Candida glabrata and loss of SET1 and H3K4 methylation increases susceptibility to azoles. In S. cerevisiae, loss of SET1 reduces the expression and function of the efflux pump PdrS, while in C. glabrata, loss of SET1 does not affect efflux pump expression or function. Furthermore, Set1 is necessary for azole-induced expression of ERG genes and maintaining proper ergosterol levels in C. glabrata. Clinical isolates lacking SET1 show reduced ERG11 expression and increased susceptibility to azoles.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Plant Sciences
Yan Wang, Shu-Yue Li, Ya-Zhong Wang, Yan He
Summary: By integrating genetic analysis, immunostaining technology, and protein-protein interaction studies, the putative factors linking DSB formation to chromosome axis were explored in maize meiosis. It was found that ZmASY1 and ZmPRD3 may work as a key module linking DSB sites to chromosome axes during DSB formation in maize.
Article
Genetics & Heredity
Prince Verma, Court K. M. Waterbury, Elizabeth M. Duncan
Summary: By analyzing the H3K4me3 signal on the planarian genome, it was found that Set1 plays a key role in creating this mark, with its depletion leading to abnormal stem cell phenotypes suggesting dysfunction of TSGs.
Article
Plant Sciences
Yazhong Wang, Yan Wang, Jie Zang, Huabang Chen, Yan He
Summary: The function of PRD1 in regulating meiotic DSB formation is conserved in maize, but its role in bipolar spindle assembly shows divergence among different plant species. ZmPRD1 is essential for DSB formation and homologous recombination in maize meiosis, but the function of PRD1 in bipolar spindle assembly during rice meiosis is not conserved in maize.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Rachel Bayley, Valerie Borel, Rhiannon J. Moss, Ellie Sweatman, Philip Ruis, Alice Ormrod, Amalia Goula, Rachel M. A. Mottram, Tyler Stanage, Graeme Hewitt, Marco Saponaro, Grant S. Stewart, Simon J. Boulton, Martin R. Higgs
Summary: The study suggests that methylation of histone H3 by SETD1A-BOD1L contributes to the recruitment of RIF1 to double-strand breaks, preventing excessive degradation of DNA ends and maintaining genome stability. This mechanism plays a crucial role in preserving telomere function and class switch recombination, and may be associated with the treatment and resistance of certain cancers.
Review
Cell Biology
Hao Song, Juanli Chen, Jin Huang, Peng Sun, Yanming Liu, Li Xu, Chuanfei Wei, Xin Mu, Xianjie Lu, Wei Wang, Nan Zhang, Miwei Shang, Mei Mo, Wei Zhang, Hui Zhao, Fabin Han
Summary: Parkinson's disease (PD) is a common neurodegenerative disorder caused by genetic, epigenetic, and environmental factors. Recent advance in genomics and epigenetics have revealed epigenetic mechanisms in PD. These epigenetic modifications include DNA methylation, post-translational histone modifications, chromatin remodeling, and RNA-based mechanisms, which regulate cellular functions in almost all cells. Epigenetic alterations are involved in multiple aspects of neuronal development and neurodegeneration in PD.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biology
Kanishk Jain, Matthew R. Marunde, Jonathan M. Burg, Susan L. Gloor, Faith M. Joseph, Karl F. Poncha, Zachary B. Gillespie, Keli L. Rodriguez, Irina K. Popova, Nathan W. Hall, Anup Vaidya, Sarah A. Howard, Hailey F. Taylor, Laylo Mukhsinova, Ugochi C. Onuoha, Emily F. Patteson, Spencer W. Cooke, Bethany C. Taylor, Ellen N. Weinzapfel, Marcus A. Cheek, Matthew J. Meiners, Geoffrey C. Fox, Kevin E. W. Namitz, Martis W. Cowles, Krzysztof Krajewski, Zu-Wen Sun, Michael S. Cosgrove, Nicolas L. Young, Michael-Christopher Keogh, Brian D. Strahl
Summary: This study reveals that H3 tail acetylation promotes nucleosomal accessibility to H3K4 methyl readers and writers, resolving the long-standing question of why H3K4me3 levels are coupled with H3 acetylation.
Review
Biochemistry & Molecular Biology
Hirotomo Takatsuka, Atsushi Shibata, Masaaki Umeda
Summary: Plant DNA is vulnerable to environmental stressors, and chromatin plays a crucial role in protecting DNA by altering its structure. Changes in chromatin modifications, regulation of gene expression, and the physical barrier function of condensed chromatin are key mechanisms for plants to maintain genome integrity under genotoxic stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sahar Waseem, Sudeep Kumar, Kanghoon Lee, Byoung-Ha Yoon, Mirang Kim, Hail Kim, Keesook Lee
Summary: PRMT1 is crucial for male fertility in mice, as its deficiency leads to impaired repair pathway of DNA double-strand breaks in male germ cells. This study provides new insight into the role of PRMT1-mediated asymmetric demethylation in mouse spermatogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Mirna Barsoum, Alexander T. Stenzel, Agnieszka Bochynska, Chao-Chung Kuo, Alexander Tsompanidis, Roksaneh Sayadi-Boroujeni, Philip Bussmann, Juliane Luescher-Firzlaff, Ivan G. Costa, Bernhard Luescher
Summary: This study investigates the impact of Ash2l loss on gene expression and cellular function. The results indicate that Ash2l loss leads to a decrease in H3K4me3 and changes in chromatin accessibility, affecting gene transcription and cellular function.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Futaba Inoue, Kenbun Sone, Yusuke Toyohara, Yu Takahashi, Asako Kukita, Aki Hara, Ayumi Taguchi, Michihiro Tanikawa, Tetsushi Tsuruga, Yutaka Osuga
Summary: Epigenetic changes play a crucial role in endometrial cancer, including DNA methylation and histone modification. There are currently inhibitors targeting epigenetic regulators in preclinical studies and clinical trials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kenichiro Matsuzaki, Miki Shinohara
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Cell Biology
Jagadeeswara Rao Bommi, Hanumanthu Bala Durga Prasada Rao, Kiran Challa, Mika Higashide, Kaori Shinmyozu, Jun-ichi Nakayama, Miki Shinohara, Akira Shinohara
Article
Genetics & Heredity
Kiran Challa, Ghanim Fajish, Miki Shinohara, Franz Klein, Susan M. Gasser, Akira Shinohara
Review
Genetics & Heredity
Kiran Challa, Miki Shinohara, Akira Shinohara
Article
Biochemistry & Molecular Biology
Hiroyuki Sasanuma, Hana Subhan M. Sakurai, Yuko Furihata, Kiran Challa, Lira Palmer, Susan M. Gasser, Miki Shinohara, Akira Shinohara
Article
Genetics & Heredity
Miki Shinohara, Douglas K. Bishop, Akira Shinohara
Article
Multidisciplinary Sciences
Shinji Hirotsune, Hiroshi Kiyonari, Mingyue Jin, Kanako Kumamoto, Kayo Yoshida, Miki Shinohara, Hitomi Watanabe, Anthony Wynshaw-Boris, Fumio Matsuzaki
SCIENTIFIC REPORTS
(2020)
Article
Biology
Zhihui Zhu, Mohammad Bani Ismail, Miki Shinohara, Akira Shinohara
Summary: Depletion of Cdc53 leads to defects in synaptonemal complex formation during meiosis while maintaining crossover formation, suggesting the uncoupling of meiotic recombination with synaptonemal complex assembly. The collaboration between Pch2 and Cdc53 is critical for synaptonemal complex assembly and meiotic recombination.
LIFE SCIENCE ALLIANCE
(2021)
Article
Biochemistry & Molecular Biology
Min-Su Lee, Mika T. Higashide, Hyungseok Choi, Ke Li, Soogil Hong, Kangseok Lee, Akira Shinohara, Miki Shinohara, Keun P. Kim
Summary: The Ecm11-Gmc2 (EG) complex plays a critical role in meiotic recombination in yeast by regulating double-strand break formation and crossover frequencies, while the SC central region is essential for controlling specificity during meiosis recombination.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Genetics & Heredity
Krishnaprasad G. Nandanan, Sagar Salim, Ajith Pankajam, Miki Shinohara, Gen Lin, Parijat Chakraborty, Amamah Farnaz, Lars M. Steinmetz, Akira Shinohara, Koodali T. Nishant
Summary: The Msh4-Msh5 complex in baker's yeast plays a vital role in meiotic crossovers and is found to associate specifically with DSB hotspots, chromosome axes, and centromeres. Its binding is enhanced by DSB formation and resection, and is correlated to DSB frequency, as well as being influenced by chromosome size and density of crossovers. These findings shed light on how Msh4-Msh5 works with other pro-crossover factors to ensure crossover formation.
Article
Biology
Hanumanthu B. D. Prasada Rao, Takeshi Sato, Kiran Challa, Yurika Fujita, Miki Shinohara, Akira Shinohara
Summary: During meiosis, CDK and DDK regulate the dynamics of Mps3 on the nuclear envelope by mediating the formation of Mps3 clusters and telomere clustering. The luminal region of Mps3 plays a crucial role in the meiosis-specific localisation of Mps3 on the nuclear envelope. Phosphorylation-dependent regulation of Mps3 localisation on the nuclear envelope is essential for meiotic chromosome motion and nuclear envelope remodelling.
Article
Multidisciplinary Sciences
Kenichiro Matsuzaki, Kenji Kumatoriya, Mizuki Tando, Takashi Kometani, Miki Shinohara
Summary: Acetaldehyde, a metabolic product of ethanol, induces DNA damage and genome instability. The repair of acetaldehyde-induced DNA damage relies on both non-homologous end-joining (NHEJ) and homology-directed repair (HDR). Additionally, polyphenols extracted from persimmon fruits can attenuate acetaldehyde-induced DNA damage.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Miki Shinohara, Akira Shinohara
Summary: Meiotic crossing over is essential for the segregation of homologous chromosomes. The formation and distribution of meiotic crossovers (COs) are tightly regulated. This study analyzed the localization of proteins involved in meiotic recombination in xrs2 hypomorphic mutants and found a proportional relationship between the number of cytological foci with recombinases and the DSB numbers. The study also revealed a chromosome-specific homeostatic response of COs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)