Human Embryonic Kidney 293 Cells: A Vehicle for Biopharmaceutical Manufacturing, Structural Biology, and Electrophysiology

Designer cells tackle diabetes

(2017) Paulina Strzyz   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Manufacturing of recombinant adeno-associated viruses using mammalian expression platforms

(2017) Marc-André Robert et al.   Biotechnology Journal

Production of recombinant coagulation factors: Are humans the best host cells?

(2017) Kamilla Swiech et al.   Bioengineered

Human Embryonic and Induced Pluripotent Stem Cell Based Toxicity Testing Models: Future Applications in New Drug Discovery

(2016) Vaibhav Shinde et al.   CURRENT MEDICINAL CHEMISTRY

Recombinant protein production from stable mammalian cell lines and pools

(2016) David L Hacker et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Toxicity study of ochratoxin A using HEK293 and HepG2 cell lines based on microRNA profiling

(2016) J Zhao et al.   HUMAN & EXPERIMENTAL TOXICOLOGY

Enhancing heterologous protein expression and secretion in HEK293 cells by means of combination of CMV promoter and IFNα2 signal peptide

(2016) Ramón Román et al.   JOURNAL OF BIOTECHNOLOGY

Production of Recombinant Adeno-associated Virus Vectors Using Suspension HEK293 Cells and Continuous Harvest of Vector From the Culture Media for GMP FIX and FLT1 Clinical Vector

(2016) Joshua C Grieger et al.   MOLECULAR THERAPY

OUP accepted manuscript

(2016)   NUCLEIC ACIDS RESEARCH

 -cell-mimetic designer cells provide closed-loop glycemic control

(2016) M. Xie et al.   SCIENCE

Hemagglutinin and neuraminidase containing virus-like particles produced in HEK-293 suspension culture: An effective influenza vaccine candidate

(2016) Alina Venereo-Sanchez et al.   VACCINE

Optimization and scale-up of cell culture and purification processes for production of an adenovirus-vectored tuberculosis vaccine candidate

(2016) Chun Fang Shen et al.   VACCINE

A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization

(2016) Amanda Ooi et al.   Frontiers in Physiology

Self-adjusting synthetic gene circuit for correcting insulin resistance

(2016) Haifeng Ye et al.   Nature Biomedical Engineering

High throughput screening technologies for ion channels

(2015) Hai-bo Yu et al.   ACTA PHARMACOLOGICA SINICA

Approaches for recombinant human factor IX production in serum-free suspension cultures

(2015) Robson Luis Ferraz do Amaral et al.   BIOTECHNOLOGY LETTERS

Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives

(2015) Jennifer Dumont et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Stable mammalian producer cell lines for structural biology

(2015) Konrad Büssow   CURRENT OPINION IN STRUCTURAL BIOLOGY

Genotoxicity of mesoporous silica nanoparticles in human embryonic kidney 293 cells

(2015) Quanan Zhang et al.   Drug Testing and Analysis

HEK293 in cell biology and cancer research: phenotype, karyotype, tumorigenicity, and stress-induced genome-phenotype evolution

(2015) A.A. Stepanenko et al.   GENE

Microcystin-LR-induced cytotoxicity and apoptosis in human embryonic kidney and human kidney adenocarcinoma cell lines

(2015) M. A. P. C. Piyathilaka et al.   MICROBIOLOGY-SGM

Human cell lines for the production of recombinant proteins: on the horizon

(2015) Lukas Fliedl et al.   New Biotechnology

Oxidative stress is involved in Patulin induced apoptosis in HEK293 cells

(2015) Baigang Zhang et al.   TOXICON

Genetic stability of a recombinant adenovirus vaccine vector seed library expressing human papillomavirus type 16 E6 and E7 proteins

(2015) JIE WU et al.   Experimental and Therapeutic Medicine

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

(2015) Ganesh P. Subedi et al.   Jove-Journal of Visualized Experiments

Cytotoxic Effect of Microbial Biosurfactants Against Human Embryonic Kidney Cancerous Cell: HEK-293 and Their Possible Role in Apoptosis

(2014) Arun Kumar Pradhan et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

The RCSB Protein Data Bank: views of structural biology for basic and applied research and education

(2014) Peter W. Rose et al.   NUCLEIC ACIDS RESEARCH

Over-expression of secreted proteins from mammalian cell lines

(2014) Annamarie C. Dalton et al.   PROTEIN SCIENCE

Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations

(2014) Yao-Cheng Lin et al.   Nature Communications

The recombinant expression systems for structure determination of eukaryotic membrane proteins

(2014) Yuan He et al.   Protein & Cell

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

(2014) Nicola Portolano et al.   Jove-Journal of Visualized Experiments

High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells

(2013) Volker Jäger et al.   BMC BIOTECHNOLOGY

Technologies to keep an eye on: alternative hosts for protein production in structural biology

(2013) Francisco J Fernández et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Biochemical, single-channel, whole-cell patch clamp, and pharmacological analyses of endogenous TRPM4 channels in HEK293 cells

(2013) Mohamed-Yassine Amarouch et al.   NEUROSCIENCE LETTERS

Production of Recombinant Therapeutic Proteins in Human Cells: Current Achievements and Future Perspectives

(2013) Virgínia Picanco-Castro et al.   PROTEIN AND PEPTIDE LETTERS

Human induced pluripotent stem cells and their use in drug discovery for toxicity testing

(2013) Clay W Scott et al.   TOXICOLOGY LETTERS

The first recombinant human coagulation factor VIII of human origin: human cell line and manufacturing characteristics

(2012) Elisabeth Casademunt et al.   EUROPEAN JOURNAL OF HAEMATOLOGY

Differences in the glycosylation of recombinant proteins expressed in HEK and CHO cells

(2012) Amelie Croset et al.   JOURNAL OF BIOTECHNOLOGY

Human cells: New platform for recombinant therapeutic protein production

(2012) Kamilla Swiech et al.   PROTEIN EXPRESSION AND PURIFICATION

Ethoxylated adjuvants of glyphosate-based herbicides are active principles of human cell toxicity

(2012) R. Mesnage et al.   TOXICOLOGY

Mammalian cell protein expression for biopharmaceutical production

(2011) Jianwei Zhu   BIOTECHNOLOGY ADVANCES

Automation of large scale transient protein expression in mammalian cells

(2011) Yuguang Zhao et al.   JOURNAL OF STRUCTURAL BIOLOGY

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

(2011) Adriano Senatore et al.   Jove-Journal of Visualized Experiments

Primary Cells and Stem Cells in Drug Discovery: Emerging Tools for High-Throughput Screening

(2010) Richard Eglen et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

The future of toxicity testing: a focus on in vitro methods using a quantitative high-throughput screening platform

(2010) Sunita J. Shukla et al.   DRUG DISCOVERY TODAY

Long peptides induce polyfunctional T cells against conserved regions of HIV-1 with superior breadth to single-gene vaccines in macaques

(2010) Maximillian Rosario et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

Recent advances in the production of proteins in insect and mammalian cells for structural biology

(2010) Joanne E. Nettleship et al.   JOURNAL OF STRUCTURAL BIOLOGY

Vaccination with Ad5 Vectors Expands Ad5-Specific CD8+ T Cells without Altering Memory Phenotype or Functionality

(2010) Natalie A. Hutnick et al.   PLoS One

Safety and Immunogenicity of a Replication-Defective Adenovirus Type 5 HIV Vaccine in Ad5-Seronegative Persons: A Randomized Clinical Trial (HVTN 054)

(2010) Laurence Peiperl et al.   PLoS One

Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods

(2010) J. van der Valk et al.   TOXICOLOGY IN VITRO

An adenoviral vector expressing human adenovirus 5 and 3 fiber proteins for targeting heterogeneous cell populations

(2010) Miho Murakami et al.   VIROLOGY

Dickkopf-1 enhances migration of HEK293 cell by beta-catenin/E-cadherin degradation

(2009) Hai-Bin Kuang   Frontiers in Bioscience-Landmark

Egr1 andGas6 facilitate the adaptation of HEK-293 cells to serum-free media by conferring enhanced viability and higher growth rates

(2007) Pratik Jaluria et al.   BIOTECHNOLOGY AND BIOENGINEERING