A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization
Published 2016 View Full Article
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Title
A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization
Authors
Keywords
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Journal
Frontiers in Physiology
Volume 7, Issue -, Pages -
Publisher
Frontiers Media SA
Online
2016-07-19
DOI
10.3389/fphys.2016.00300
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- Mild Hypothermia Improves Transient Gene Expression Yields Several Fold in Chinese Hamster Ovary Cells
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