The recombinant expression systems for structure determination of eukaryotic membrane proteins

Nuclear Membranes GPCRs: Implication in Cardiovascular Health and Diseases

(2014) Ghassan Bkaily et al.   Current Vascular Pharmacology

Crystal structure of the human glucose transporter GLUT1

(2014) Dong Deng et al.   NATURE

Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1

(2014) Joanne L. Parker et al.   NATURE

Crystal structure of the plant dual-affinity nitrate transporter NRT1.1

(2014) Ji Sun et al.   NATURE

Structure and mechanism of a eukaryotic transmembrane ascorbate-dependent oxidoreductase

(2014) Peilong Lu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of a Class C GPCR Metabotropic Glutamate Receptor 1 Bound to an Allosteric Modulator

(2014) H. Wu et al.   SCIENCE

Crystal Structure of a Claudin Provides Insight into the Architecture of Tight Junctions

(2014) H. Suzuki et al.   SCIENCE

Protein production using the baculovirus-insect cell expression system

(2013) A. Contreras-Gómez et al.   BIOTECHNOLOGY PROGRESS

Structure of class B GPCR corticotropin-releasing factor receptor 1

(2013) Kaspar Hollenstein et al.   NATURE

Crystal structure of a eukaryotic phosphate transporter

(2013) Bjørn P. Pedersen et al.   NATURE

TRPV1 structures in distinct conformations reveal activation mechanisms

(2013) Erhu Cao et al.   NATURE

Structure of the human smoothened receptor bound to an antitumour agent

(2013) Chong Wang et al.   NATURE

Structure of the TRPV1 ion channel determined by electron cryo-microscopy

(2013) Maofu Liao et al.   NATURE

X-ray structure of dopamine transporter elucidates antidepressant mechanism

(2013) Aravind Penmatsa et al.   NATURE

Structure of the human glucagon class B G-protein-coupled receptor

(2013) Fai Yiu Siu et al.   NATURE

Structures of ABCB10, a human ATP-binding cassette transporter in apo- and nucleotide-bound states

(2013) C. A. Shintre et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of the CCR5 Chemokine Receptor-HIV Entry Inhibitor Maraviroc Complex

(2013) Q. Tan et al.   SCIENCE

Structural Basis for Molecular Recognition at Serotonin Receptors

(2013) C. Wang et al.   SCIENCE

Structural Features for Functional Selectivity at Serotonin Receptors

(2013) D. Wacker et al.   SCIENCE

The Structural Basis of ZMPSTE24-Dependent Laminopathies

(2013) A. Quigley et al.   SCIENCE

Structure of the Integral Membrane Protein CAAX Protease Ste24p

(2013) E. E. Pryor et al.   SCIENCE

Structure of the δ-opioid receptor bound to naltrindole

(2012) Sébastien Granier et al.   NATURE

Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist

(2012) Kazuko Haga et al.   NATURE

Structure of the human κ-opioid receptor in complex with JDTic

(2012) Huixian Wu et al.   NATURE

Structure of the nociceptin/orphanin FQ receptor in complex with a peptide mimetic

(2012) Aaron A. Thompson et al.   NATURE

Structure of the agonist-bound neurotensin receptor

(2012) Jim F. White et al.   NATURE

Crystal structure of the µ-opioid receptor bound to a morphinan antagonist

(2012) Aashish Manglik et al.   NATURE

Crystal structure of a membrane-embedded H+-translocating pyrophosphatase

(2012) Shih-Ming Lin et al.   NATURE

Structural plasticity and dynamic selectivity of acid-sensing ion channel–spider toxin complexes

(2012) Isabelle Baconguis et al.   NATURE

Structure and dynamics of the M3 muscarinic acetylcholine receptor

(2012) Andrew C. Kruse et al.   NATURE

Molecular mechanism of ATP binding and ion channel activation in P2X receptors

(2012) Motoyuki Hattori et al.   NATURE

Crystal structure of the multidrug transporter P-glycoprotein from Caenorhabditis elegans

(2012) Mi Sun Jin et al.   NATURE

High-resolution crystal structure of human protease-activated receptor 1

(2012) Cheng Zhang et al.   NATURE

Structure and permeation mechanism of a mammalian urea transporter

(2012) E. J. Levin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal Structure of a Lipid G Protein-Coupled Receptor

(2012) M. A. Hanson et al.   SCIENCE

Crystal Structure of the Human K2P TRAAK, a Lipid- and Mechano-Sensitive K+ Ion Channel

(2012) S. G. Brohawn et al.   SCIENCE

Crystal Structure of the Human Two-Pore Domain Potassium Channel K2P1

(2012) A. N. Miller et al.   SCIENCE

Crystal Structure of the Calcium Release-Activated Calcium Channel Orai

(2012) X. Hou et al.   SCIENCE

Experiences in fragment-based drug discovery

(2012) Christopher W. Murray et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Mammalian cell protein expression for biopharmaceutical production

(2011) Jianwei Zhu   BIOTECHNOLOGY ADVANCES

Crystal Structure of the Mammalian GIRK2 K+ Channel and Gating Regulation by G Proteins, PIP2, and Sodium

(2011) Matthew R. Whorton et al.   CELL

Structure of the human histamine H1 receptor complex with doxepin

(2011) Tatsuro Shimamura et al.   NATURE

Principles of activation and permeation in an anion-selective Cys-loop receptor

(2011) Ryan E. Hibbs et al.   NATURE

The crystal structure of a voltage-gated sodium channel

(2011) Jian Payandeh et al.   NATURE

Structure of a nanobody-stabilized active state of the β2 adrenoceptor

(2011) Søren G. F. Rasmussen et al.   NATURE

Structure and function of an irreversible agonist-β2 adrenoceptor complex

(2011) Daniel M. Rosenbaum et al.   NATURE

Crystal structure of the β2 adrenergic receptor–Gs protein complex

(2011) Søren G. F. Rasmussen et al.   NATURE

Overcoming barriers to membrane protein structure determination

(2011) Roslyn M Bill et al.   NATURE BIOTECHNOLOGY

Heterologous Expression of Membrane Proteins: Choosing the Appropriate Host

(2011) Florent Bernaudat et al.   PLoS One

Strategies for the enhancement of recombinant protein production from mammalian cells by growth arrest

(2010) Kevin Sunley et al.   BIOTECHNOLOGY ADVANCES

Maltose–neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins

(2010) Pil Seok Chae et al.   NATURE METHODS

Function of human Rh based on structure of RhCG at 2.1 A

(2010) F. Gruswitz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structures of the CXCR4 Chemokine GPCR with Small-Molecule and Cyclic Peptide Antagonists

(2010) B. Wu et al.   SCIENCE

Structure of the Human Dopamine D3 Receptor in Complex with a D2/D3 Selective Antagonist

(2010) E. Y. T. Chien et al.   SCIENCE

Structure of a Eukaryotic CLC Transporter Defines an Intermediate State in the Transport Cycle

(2010) Liang Feng et al.   SCIENCE

A Gating Charge Transfer Center in Voltage Sensors

(2010) X. Tao et al.   SCIENCE

High human GLUT1, GLUT2, and GLUT3 expression in Schizosaccharomyces pombe

(2009) Yuxin Yang et al.   BIOCHEMISTRY-MOSCOW

Methanol-inducible gene expression and heterologous protein production in the methylotrophic yeast Candida boidinii

(2009) Hiroya Yurimoto et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Pore architecture and ion sites in acid-sensing ion channels and P2X receptors

(2009) Eric B. Gonzales et al.   NATURE

X-ray structure, symmetry and mechanism of an AMPA-subtype glutamate receptor

(2009) Alexander I. Sobolevsky et al.   NATURE

Crystal structure of the ATP-gated P2X4 ion channel in the closed state

(2009) Toshimitsu Kawate et al.   NATURE

Emerging roles for lipids in shaping membrane-protein function

(2009) Rob Phillips et al.   NATURE

Structure of the connexin 26 gap junction channel at 3.5 Å resolution

(2009) Shoji Maeda et al.   NATURE

Crystallizing membrane proteins using lipidic mesophases

(2009) Martin Caffrey et al.   Nature Protocols

Crystal structure of human aquaporin 4 at 1.8 A and its mechanism of conductance

(2009) J. D. Ho et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal Structure of the Eukaryotic Strong Inward-Rectifier K+ Channel Kir2.2 at 3.1 A Resolution

(2009) X. Tao et al.   SCIENCE

Structure of P-Glycoprotein Reveals a Molecular Basis for Poly-Specific Drug Binding

(2009) S. G. Aller et al.   SCIENCE

Structure of a β1-adrenergic G-protein-coupled receptor

(2008) Tony Warne et al.   NATURE

Membrane lipids: where they are and how they behave

(2008) Gerrit van Meer et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Crystal structure of the aquaglyceroporin PfAQP from the malarial parasite Plasmodium falciparum

(2008) Zachary E R Newby et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

High-resolution x-ray structure of human aquaporin 5

(2008) R. Horsefield et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study

(2008) Nicola A. Burgess-Brown et al.   PROTEIN EXPRESSION AND PURIFICATION

BacMam system for high-level expression of recombinant soluble and membrane glycoproteins for structural studies

(2008) Abhiram Dukkipati et al.   PROTEIN EXPRESSION AND PURIFICATION

The 2.6 Angstrom Crystal Structure of a Human A2A Adenosine Receptor Bound to an Antagonist

(2008) V.-P. Jaakola et al.   SCIENCE