Methanol independent induction in Pichia pastoris by simple derepressed overexpression of single transcription factors

Novel function of Wsc proteins as a methanol-sensing machinery in the yeastPichia pastoris

(2017) Shin Ohsawa et al.   MOLECULAR MICROBIOLOGY

Plant-Derived Transcription Factors for Orthologous Regulation of Gene Expression in the Yeast Saccharomyces cerevisiae

(2017) Gita Naseri et al.   ACS Synthetic Biology

Positive Feedback Genetic Circuit Incorporating a Constitutively Active Mutant Gal3 into Yeast GAL Induction System

(2017) Shintaro Ryo et al.   ACS Synthetic Biology

Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris

(2017) Jinjia Wang et al.   Scientific Reports

Enzyme discovery beyond homology: a unique hydroxynitrile lyase in the Bet v1 superfamily

(2017) Elisa Lanfranchi et al.   Scientific Reports

Coordinated transcription factor and promoter engineering to establish strong expression elements inSaccharomyces cerevisiae

(2016) John M. Leavitt et al.   Biotechnology Journal

Massively Parallel Interrogation of the Effects of Gene Expression Levels on Fitness

(2016) Leeat Keren et al.   CELL

Large-scale mapping of gene regulatory logic reveals context-dependent repression by transcriptional activators

(2016) David van Dijk et al.   GENOME RESEARCH

Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 (AOX1) Promoter inPichia pastoris

(2016) Xiaolong Wang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization

(2016) Jan-Philipp Schwarzhans et al.   Microbial Cell Factories

Pichia pastoris MutS strains are prone to misincorporation of O-methyl-l-homoserine at methionine residues when methanol is used as the sole carbon source

(2016) Peter Schotte et al.   Microbial Cell Factories

A novel methanol-free Pichia pastoris system for recombinant protein expression

(2016) Wei Shen et al.   Microbial Cell Factories

Coordinated transcription factor and promoter engineering to establish strong expression elements inSaccharomyces cerevisiae

(2016) John M. Leavitt et al.   Biotechnology Journal

Synthetic Transcription Amplifier System for Orthogonal Control of Gene Expression in Saccharomyces cerevisiae

(2016) Anssi Rantasalo et al.   PLoS One

Kinase Screening in Pichia pastoris Identified Promising Targets Involved in Cell Growth and Alcohol Oxidase 1 Promoter (PAOX1) Regulation

(2016) Wei Shen et al.   PLoS One

Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing

(2016) Jan-Philipp Schwarzhans et al.   Scientific Reports

Compact multi-enzyme pathways in P. pastoris

(2015) Martina Geier et al.   CHEMICAL COMMUNICATIONS

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris

(2015) Thomas Vogl et al.   ACS Synthetic Biology

Synergistic modular promoter and gene optimization to push cellulase secretion by Pichia pastoris beyond existing benchmarks

(2014) Andrea Mellitzer et al.   JOURNAL OF BIOTECHNOLOGY

Carbon source dependent promoters in yeasts

(2014) Katrin Weinhandl et al.   Microbial Cell Factories

Towards improved membrane protein production in Pichia pastoris: General and specific transcriptional response to membrane protein overexpression

(2014) Thomas Vogl et al.   New Biotechnology

Inducible, tightly regulated and growth condition-independent transcription factor in Saccharomyces cerevisiae

(2014) Diana S.M. Ottoz et al.   NUCLEIC ACIDS RESEARCH

Improving functional annotation for industrial microbes: a case study with Pichia pastoris

(2014) Duygu Dikicioglu et al.   TRENDS IN BIOTECHNOLOGY

Playing catch-up with Escherichia coli: using yeast to increase success rates in recombinant protein production experiments

(2014) Roslyn M. Bill   Frontiers in Microbiology

Small-scale slow glucose feed cultivation of Pichia pastoris without repression of AOX1 promoter: towards high throughput cultivations

(2013) Johanna Panula-Perälä et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris

(2013) Thomas Vogl et al.   CURRENT OPINION IN BIOTECHNOLOGY

Transferring a synthetic gene circuit from yeast to mammalian cells

(2013) Dmitry Nevozhay et al.   Nature Communications

Synthetic Core Promoters for Pichia pastoris

(2013) Thomas Vogl et al.   ACS Synthetic Biology

Controlling promoter strength and regulation inSaccharomyces cerevisiaeusing synthetic hybrid promoters

(2012) John Blazeck et al.   BIOTECHNOLOGY AND BIOENGINEERING

Promoter engineering: Recent advances in controlling transcription at the most fundamental level

(2012) John Blazeck et al.   Biotechnology Journal

A Synthetic Biology Framework for Programming Eukaryotic Transcription Functions

(2012) Ahmad S. Khalil et al.   CELL

Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

(2012) Florian W Krainer et al.   Microbial Cell Factories

Pichia pastoris 14-3-3 regulates transcriptional activity of the methanol inducible transcription factor Mxr1 by direct interaction

(2012) Pabitra K. Parua et al.   MOLECULAR MICROBIOLOGY

Regulation of Pichia pastoris promoters and its consequences for protein production

(2012) Thomas Vogl et al.   New Biotechnology

Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

(2012) Laura Näätsaari et al.   PLoS One

Molecular characterization of Candida boidinii MIG1 and its role in the regulation of methanol-inducible gene expression

(2012) Zhenyu Zhai et al.   YEAST

Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae

(2011) Nancy A. Da Silva et al.   FEMS YEAST RESEARCH

Transcriptional Regulation inSaccharomyces cerevisiae: Transcription Factor Regulation and Function, Mechanisms of Initiation, and Roles of Activators and Coactivators

(2011) Steven Hahn et al.   GENETICS

14-3-3 (Bmh) Proteins Inhibit Transcription Activation by Adr1 through Direct Binding to Its Regulatory Domain

(2010) P. K. Parua et al.   MOLECULAR AND CELLULAR BIOLOGY

A systematical investigation on the genetic stability of multi-copy Pichia pastoris strains

(2009) Taicheng Zhu et al.   BIOTECHNOLOGY LETTERS

Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression

(2009) D. Nevozhay et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Viral vectors based on bidirectional cell-specific mammalian promoters and transcriptional amplification strategy for use in vitro and in vivo

(2008) Beihui Liu et al.   BMC BIOTECHNOLOGY

Trm1p, a Zn(II)2Cys6-Type Transcription Factor, Is a Master Regulator of Methanol-Specific Gene Activation in the Methylotrophic YeastCandida boidinii

(2008) Yu Sasano et al.   EUKARYOTIC CELL

Promoter library designed for fine-tuned gene expression in Pichia pastoris

(2008) Franz S. Hartner et al.   NUCLEIC ACIDS RESEARCH