Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris

Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 (AOX1) Promoter inPichia pastoris

(2016) Xiaolong Wang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Disruption of KEX1 gene reduces the proteolytic degradation of secreted two-chain Insulin glargine in Pichia pastoris

(2016) Suma Sreenivas et al.   PROTEIN EXPRESSION AND PURIFICATION

Cultivation strategies to enhance productivity of Pichia pastoris: A review

(2015) V. Looser et al.   BIOTECHNOLOGY ADVANCES

PpNrg1 is a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris

(2015) Xiaolong Wang et al.   BIOTECHNOLOGY LETTERS

Multiple copies of the target gene enhances plectasin secretion in Pichia pastoris X-33

(2015) Da Teng et al.   PROCESS BIOCHEMISTRY

Trm1p, a Zn(II)2Cys6-type transcription factor, is essential for the transcriptional activation of genes of methanol utilization pathway, in Pichia pastoris

(2014) Umakant Sahu et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures

(2014) Ana Vanz et al.   Microbial Cell Factories

Nrg1 functions as a global transcriptional repressor of glucose-repressed genes through its direct binding to the specific promoter regions

(2013) Sung Bae Lee et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Pichia pastoris: protein production host and model organism for biomedical research

(2013) Brigitte Gasser et al.   Future Microbiology

Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris

(2013) Roland Prielhofer et al.   Microbial Cell Factories

Production of recombinant Aedes albopictus akirin in Pichia pastoris using an aqueous two-phase semicontinuous fermentation process

(2012) Juan A. Moreno-Cid et al.   BIOCHEMICAL ENGINEERING JOURNAL

Gss1 protein of the methylotrophic yeast Pichia pastoris is involved in glucose sensing, pexophagy and catabolite repression

(2012) Andriy S. Polupanov et al.   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

Regulation of Pichia pastoris promoters and its consequences for protein production

(2012) Thomas Vogl et al.   New Biotechnology

Molecular characterization of Candida boidinii MIG1 and its role in the regulation of methanol-inducible gene expression

(2012) Zhenyu Zhai et al.   YEAST

Production of recombinant proteins by yeast cells

(2011) Eda Çelik et al.   BIOTECHNOLOGY ADVANCES

Catabolite Repression of Aox in Pichia pastoris Is Dependent on Hexose Transporter PpHxt1 and Pexophagy

(2010) P. Zhang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin

(2010) Chandrasekhar Gurramkonda et al.   Microbial Cell Factories

Identification of Mxr1p-binding sites in the promoters of genes encoding dihydroxyacetone synthase and peroxin 8 of the methylotrophic yeast Pichia pastoris

(2010) Balla Venkata Kranthi et al.   YEAST

Phosphate-Responsive Promoter of a Pichia pastoris Sodium Phosphate Symporter

(2009) J. Ahn et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Identification of key DNA elements involved in promoter recognition by Mxr1p, a master regulator of methanol utilization pathway in Pichia pastoris

(2009) Balla Venkata Kranthi et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

An upstream activation sequence controls the expression ofAOX1gene inPichia pastoris

(2009) Yaoji Xuan et al.   FEMS YEAST RESEARCH

Efficient generation of multi-copy strains for optimizing secretory expression of porcine insulin precursor in yeastPichia pastoris

(2009) T. Zhu et al.   JOURNAL OF APPLIED MICROBIOLOGY

Genome sequence of the recombinant protein production host Pichia pastoris

(2009) Kristof De Schutter et al.   NATURE BIOTECHNOLOGY

Trm1p, a Zn(II)2Cys6-Type Transcription Factor, Is a Master Regulator of Methanol-Specific Gene Activation in the Methylotrophic YeastCandida boidinii

(2008) Yu Sasano et al.   EUKARYOTIC CELL

Recent advances on the GAP promoter derived expression system of Pichia pastoris

(2008) Ai-Lian Zhang et al.   MOLECULAR BIOLOGY REPORTS

Analyzing real-time PCR data by the comparative CT method

(2008) Thomas D Schmittgen et al.   Nature Protocols

Promoter library designed for fine-tuned gene expression in Pichia pastoris

(2008) Franz S. Hartner et al.   NUCLEIC ACIDS RESEARCH

Secretory Expression of Insulin Precursor inPichia pastorisand Simple Procedure for Producing Recombinant Human Insulin

(2008) T. Xie et al.   PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY