Journal
BIOTECHNOLOGY JOURNAL
Volume 8, Issue 1, Pages 46-+Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201200120
Keywords
Bioengineering; Gene expression; Metabolic engineering; Synthetic biology
Funding
- DuPont Young Professor Grant
- Office of Naval Research Young Investigator Program Award
- National Institute of General Medical Sciences of the National Institutes of Health [R01GM090221]
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Synthetic control of gene expression is critical for metabolic engineering efforts. Specifically, precise control of key pathway enzymes (heterologous or native) can help maximize product formation. The fundamental level of transcriptional control takes place at promoter elements that drive gene expression. Endogenous promoters are limited in that they do not fully sample the complete continuum of transcriptional control, and do not maximize the transcription levels achievable within an organism. To address this issue, several attempts at promoter engineering have shown great promise both in expanding the cell-wide transcriptional capacity of an organism and in enabling tunable levels of gene expression. Thus, this review highlights the recent advances and approaches for altering gene expression control at the promoter level. Furthermore, we propose that recent advances in the understanding of transcription factors and their DNA-binding sites will enable rational and predictive control of gene expression.
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