Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris

(2015) Thomas Vogl et al.   ACS Synthetic Biology

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Biopharmaceutical discovery and production in yeast

(2014) Michael A Meehl et al.   CURRENT OPINION IN BIOTECHNOLOGY

Complete genome sequence of the methanogenic neotype strain Methanobacterium formicicum MFT

(2014) Irena Maus et al.   JOURNAL OF BIOTECHNOLOGY

Increased homologous integration frequency in Yarrowia lipolytica strains defective in non-homologous end-joining

(2013) Anne Kretzschmar et al.   CURRENT GENETICS

Pichia pastoris: protein production host and model organism for biomedical research

(2013) Brigitte Gasser et al.   Future Microbiology

Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris

(2013) Roland Prielhofer et al.   Microbial Cell Factories

Can too many copies spoil the broth?

(2013) Rochelle Aw et al.   Microbial Cell Factories

Enhancement of the Gene Targeting Efficiency of Non-Conventional Yeasts by Increasing Genetic Redundancy

(2013) Zao Chen et al.   PLoS One

Synthetic Core Promoters for Pichia pastoris

(2013) Thomas Vogl et al.   ACS Synthetic Biology

The complete genome sequence of the acarbose producer Actinoplanes sp. SE50/110

(2012) Patrick Schwientek et al.   BMC GENOMICS

Genome-scale metabolic reconstructions of Pichia stipitis and Pichia pastoris and in-silico evaluation of their potentials

(2012) Luis Caspeta et al.   BMC Systems Biology

The Zinc Finger Proteins Mxr1p and Repressor of Phosphoenolpyruvate Carboxykinase (ROP) Have the Same DNA Binding Specificity but Regulate Methanol Metabolism Antagonistically inPichia pastoris

(2012) Nallani Vijay Kumar et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Insights into the completely annotated genome of Lactobacillus buchneri CD034, a strain isolated from stable grass silage

(2012) Stefan Heinl et al.   JOURNAL OF BIOTECHNOLOGY

Establishment and interpretation of the genome sequence of the phytopathogenic fungus Rhizoctonia solani AG1-IB isolate 7/3/14

(2012) Daniel Wibberg et al.   JOURNAL OF BIOTECHNOLOGY

Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

(2012) Florian W Krainer et al.   Microbial Cell Factories

Pichia pastoris 14-3-3 regulates transcriptional activity of the methanol inducible transcription factor Mxr1 by direct interaction

(2012) Pabitra K. Parua et al.   MOLECULAR MICROBIOLOGY

Regulation of Pichia pastoris promoters and its consequences for protein production

(2012) Thomas Vogl et al.   New Biotechnology

Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

(2012) Laura Näätsaari et al.   PLoS One

Novel genetic tools for Hansenula polymorpha

(2011) Ruchi Saraya et al.   FEMS YEAST RESEARCH

Complete genome sequencing of Agrobacterium sp. H13-3, the former Rhizobium lupini H13-3, reveals a tripartite genome consisting of a circular and a linear chromosome and an accessory plasmid but lacking a tumor-inducing Ti-plasmid

(2011) Daniel Wibberg et al.   JOURNAL OF BIOTECHNOLOGY

High-quality genome sequence of Pichia pastoris CBS7435

(2011) Andreas Küberl et al.   JOURNAL OF BIOTECHNOLOGY

Real-time PCR-based determination of gene copy numbers inPichia pastoris

(2010) Sandra Abad et al.   Biotechnology Journal

Genome-scale metabolic model of methylotrophic yeastPichia pastorisand its use forin silicoanalysis of heterologous protein production

(2010) Seung Bum Sohn et al.   Biotechnology Journal

Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production

(2010) Gerhard Stadlmayr et al.   JOURNAL OF BIOTECHNOLOGY

Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement

(2010) Bevan KS Chung et al.   Microbial Cell Factories

Genome-scale analysis of library sorting (GALibSo): Isolation of secretion enhancing factors for recombinant protein production inPichia pastoris

(2009) Gerhard Stadlmayr et al.   BIOTECHNOLOGY AND BIOENGINEERING

A systematical investigation on the genetic stability of multi-copy Pichia pastoris strains

(2009) Taicheng Zhu et al.   BIOTECHNOLOGY LETTERS

Directed gene copy number amplification inPichia pastorisby vector integration into the ribosomal DNA locus

(2009) Hans Marx et al.   FEMS YEAST RESEARCH

Efficient generation of multi-copy strains for optimizing secretory expression of porcine insulin precursor in yeastPichia pastoris

(2009) T. Zhu et al.   JOURNAL OF APPLIED MICROBIOLOGY

Genome sequence of the recombinant protein production host Pichia pastoris

(2009) Kristof De Schutter et al.   NATURE BIOTECHNOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Promoter library designed for fine-tuned gene expression in Pichia pastoris

(2008) Franz S. Hartner et al.   NUCLEIC ACIDS RESEARCH