The CRISPR/Cas9 system sheds new lights on the biology of protozoan parasites

CRISPR-Cas9-based genome-wide screening of Toxoplasma gondii

(2018) Saima M Sidik et al.   Nature Protocols

CRISPR/Cas9-mediated gene modification and gene knock out in the human-infective parasite Trichomonas vaginalis

(2018) Brian D. Janssen et al.   Scientific Reports

Gene editing and clonal isolation of human induced pluripotent stem cells using CRISPR/Cas9

(2017) Saniye Yumlu et al.   METHODS

Epigenetics of cell fate reprogramming and its implications for neurological disorders modelling

(2017) Maciej Grzybek et al.   NEUROBIOLOGY OF DISEASE

Plasmid-free CRISPR/Cas9 genome editing in Plasmodium falciparum confirms mutations conferring resistance to the dihydroisoquinolone clinical candidate SJ733

(2017) Emily D. Crawford et al.   PLoS One

Concise Review: Induced Pluripotent Stem Cell-Based Drug Discovery for Mitochondrial Disease

(2017) Gizem Inak et al.   STEM CELLS

Toxoplasma gondii : One Organism, Multiple Models

(2017) Edina K. Szabo et al.   TRENDS IN PARASITOLOGY

Different Roles of Mitochondrial Calcium Uniporter Complex Subunits in Growth and Infectivity of Trypanosoma cruzi

(2017) Miguel A. Chiurillo et al.   mBio

Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins

(2017) Lia Carolina Soares Medeiros et al.   mBio

A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids

(2017) Tom Beneke et al.   Royal Society Open Science

A decade of discovery: CRISPR functions and applications

(2017) Rodolphe Barrangou et al.   Nature Microbiology

CRISPR/Cas9 in Genome Editing and Beyond

(2016) Haifeng Wang et al.   Annual Review of Biochemistry

A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes

(2016) Saima M. Sidik et al.   CELL

CRISPR/Cas9-The ultimate weapon to battle infectious diseases?

(2016) M. Doerflinger et al.   CELLULAR MICROBIOLOGY

Development of whole sporozoite malaria vaccines

(2016) Michael R. Hollingdale et al.   Expert Review of Vaccines

CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering

(2016) D. J. Dickinson et al.   GENETICS

CRISPR/Cas9-mediated endogenous C-terminal Tagging ofTrypanosoma cruziGenes Reveals the Acidocalcisome Localization of the Inositol 1,4,5-Trisphosphate Receptor

(2016) Noelia Lander et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Genome Editing by CRISPR/Cas9: A Game Change in the Genetic Manipulation of Protists

(2016) Noelia Lander et al.   JOURNAL OF EUKARYOTIC MICROBIOLOGY

Dihydroartemisinin–piperaquine resistance in Plasmodium falciparum malaria in Cambodia: a multisite prospective cohort study

(2016) Chanaki Amaratunga et al.   LANCET INFECTIOUS DISEASES

CRISPR-Cas9-modifiedpfmdr1protectsPlasmodium falciparumasexual blood stages and gametocytes against a class of piperazine-containing compounds but potentiates artemisinin-based combination therapy partner drugs

(2016) Caroline L. Ng et al.   MOLECULAR MICROBIOLOGY

UAP56 is a conserved crucial component of a divergent mRNA export pathway inToxoplasma gondii

(2016) Mariana Serpeloni et al.   MOLECULAR MICROBIOLOGY

CRISPR gene-editing tested in a person for the first time

(2016) David Cyranoski   NATURE

The Past, Present, and Future of Genetic Manipulation in Toxoplasma gondii

(2016) Jin-Lei Wang et al.   TRENDS IN PARASITOLOGY

CRISPR-Cas: New Tools for Genetic Manipulations from Bacterial Immunity Systems

(2015) Wenyan Jiang et al.   Annual Review of Microbiology

First efficient CRISPR-Cas9-mediated genome editing inLeishmaniaparasites

(2015) Lauriane Sollelis et al.   CELLULAR MICROBIOLOGY

Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs

(2015) Linlin Yin et al.   GENETICS

A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for cryptosporidium

(2015) William Checkley et al.   LANCET INFECTIOUS DISEASES

Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells

(2015) Van Trung Chu et al.   NATURE BIOTECHNOLOGY

Evaluation of the basic functions of six calcium-dependent protein kinases in Toxoplasma gondii using CRISPR-Cas9 system

(2015) Jin-Lei Wang et al.   PARASITOLOGY RESEARCH

Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular dystrophy

(2015) C. Long et al.   SCIENCE

CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani

(2015) Wen-Wei Zhang et al.   mBio

CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 Genes in Trypanosoma cruzi Reveals Their Role in Flagellar Attachment

(2015) Noelia Lander et al.   mBio

Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells

(2015) Hsin-Kai Liao et al.   Nature Communications

Rhoptry Proteins ROP5 and ROP18 Are Major Murine Virulence Factors in Genetically Divergent South American Strains of Toxoplasma gondii

(2015) Michael S. Behnke et al.   PLoS Genetics

Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs

(2015) Linlin Yin et al.   GENETICS

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

CRISPR-Cas Systems: Prokaryotes Upgrade to Adaptive Immunity

(2014) Rodolphe Barrangou et al.   MOLECULAR CELL

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

(2014) Mehdi Ghorbal et al.   NATURE BIOTECHNOLOGY

Efficient CRISPR-Cas9–mediated genome editing in Plasmodium falciparum

(2014) Jeffrey C Wagner et al.   NATURE METHODS

Efficient Genome Engineering of Toxoplasma gondii Using CRISPR/Cas9

(2014) Saima M. Sidik et al.   PLoS One

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi

(2014) D. Peng et al.   mBio

Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9

(2014) B. Shen et al.   mBio

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients

(2013) Gerald Schwank et al.   Cell Stem Cell

Knockout of the abundantTrichomonas vaginalishydrogenosomal membrane proteinTvHMP23 increases hydrogenosome size but induces no compensatory up-regulation of paralogous copies

(2013) Xavier Pereira Brás et al.   FEBS LETTERS

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

(2013) Scott J. Gratz et al.   GENETICS

Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study

(2013) Karen L Kotloff et al.   LANCET

Parasitic infections: Time to tackle cryptosporidiosis

(2013) Boris Striepen   NATURE

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Global epidemiology ofTrichomonas vaginalis

(2013) Danielle N Poole et al.   SEXUALLY TRANSMITTED INFECTIONS

The CRISPRs, They Are A-Changin': How Prokaryotes Generate Adaptive Immunity

(2012) Edze R. Westra et al.   Annual Review of Genetics

Site-specific genome editing in Plasmodium falciparum using engineered zinc-finger nucleases

(2012) Judith Straimer et al.   NATURE METHODS

Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli

(2012) Ido Yosef et al.   NUCLEIC ACIDS RESEARCH

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system

(2012) Kirill A. Datsenko et al.   Nature Communications

Contribution of Enteric Infection, Altered Intestinal Barrier Function, and Maternal Malnutrition to Infant Malnutrition in Bangladesh

(2011) D. Mondal et al.   CLINICAL INFECTIOUS DISEASES

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Evolution and classification of the CRISPR–Cas systems

(2011) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

The Mechanism of Double-Strand DNA Break Repair by the Nonhomologous DNA End-Joining Pathway

(2010) Michael R. Lieber   Annual Review of Biochemistry

Phosphorylation of Immunity-Related GTPases by a Toxoplasma gondii-Secreted Kinase Promotes Macrophage Survival and Virulence

(2010) Sarah J. Fentress et al.   Cell Host & Microbe

Leishmaniasis, an emerging infection in travelers

(2010) Androula Pavli et al.   INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES

Microhomology-mediated deletion and gene conversion in African trypanosomes

(2010) Lucy Glover et al.   NUCLEIC ACIDS RESEARCH

Global epidemiology and control of Trichomonas vaginalis

(2009) Victoria J Johnston et al.   CURRENT OPINION IN INFECTIOUS DISEASES

Trichomoniasis in teens: an update

(2009) Jill S Huppert   CURRENT OPINION IN OBSTETRICS & GYNECOLOGY

Global trends in emerging infectious diseases

(2008) Kate E. Jones et al.   NATURE

Sequence homology and microhomology dominate chromosomal double-strand break repair in African trypanosomes

(2008) L. Glover et al.   NUCLEIC ACIDS RESEARCH