A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids

A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes

(2016) Saima M. Sidik et al.   CELL

CRISPR/Cas9-mediated endogenous C-terminal Tagging ofTrypanosoma cruziGenes Reveals the Acidocalcisome Localization of the Inositol 1,4,5-Trisphosphate Receptor

(2016) Noelia Lander et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Genome Editing by CRISPR/Cas9: A Game Change in the Genetic Manipulation of Protists

(2016) Noelia Lander et al.   JOURNAL OF EUKARYOTIC MICROBIOLOGY

VEX1 controls the allelic exclusion required for antigenic variation in trypanosomes

(2016) Lucy Glover et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

First efficient CRISPR-Cas9-mediated genome editing inLeishmaniaparasites

(2015) Lauriane Sollelis et al.   CELLULAR MICROBIOLOGY

Genetic modification of the diarrhoeal pathogen Cryptosporidium parvum

(2015) Sumiti Vinayak et al.   NATURE

CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani

(2015) Wen-Wei Zhang et al.   mBio

CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 Genes in Trypanosoma cruzi Reveals Their Role in Flagellar Attachment

(2015) Noelia Lander et al.   mBio

Basal body multipotency and axonemal remodelling are two pathways to a 9+0 flagellum

(2015) R. J. Wheeler et al.   Nature Communications

A toolkit enabling efficient, scalable and reproducible gene tagging in trypanosomatids

(2015) S. Dean et al.   Open Biology

Comparative Life Cycle Transcriptomics Revises Leishmania mexicana Genome Annotation and Links a Chromosome Duplication with Parasitism of Vertebrates

(2015) Michael Fiebig et al.   PLoS Pathogens

Kharon1 Null Mutants of Leishmania mexicana Are Avirulent in Mice and Exhibit a Cytokinesis Defect within Macrophages

(2015) Khoa D. Tran et al.   PLoS One

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

CRISPR/Cas9 mediated genome engineering in Drosophila

(2014) Andrew Bassett et al.   METHODS

Tetracycline-inducible gene expression system in Leishmania mexicana

(2014) Natalya Kraeva et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

(2014) Mehdi Ghorbal et al.   NATURE BIOTECHNOLOGY

Efficient CRISPR-Cas9–mediated genome editing in Plasmodium falciparum

(2014) Jeffrey C Wagner et al.   NATURE METHODS

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Efficient Editing of Malaria Parasite Genome Using the CRISPR/Cas9 System

(2014) C. Zhang et al.   mBio

CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi

(2014) D. Peng et al.   mBio

Genome-wide dissection of the quorum sensing signalling pathway in Trypanosoma brucei

(2013) Binny M. Mony et al.   NATURE

A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum

(2013) Nathan C Shaner et al.   NATURE METHODS

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Determinants of GPI-PLC Localisation to the Flagellum and Access to GPI-Anchored Substrates in Trypanosomes

(2013) Jack Sunter et al.   PLoS Pathogens

Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate

(2012) Mary P. Hall et al.   ACS Chemical Biology

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells

(2012) Kyle J. Roux et al.   JOURNAL OF CELL BIOLOGY

High-throughput decoding of antitrypanosomal drug efficacy and resistance

(2012) Sam Alsford et al.   NATURE

A modular and optimized single marker system for generating Trypanosoma brucei cell lines expressing T7 RNA polymerase and the tetracycline repressor

(2012) S. K. Poon et al.   Open Biology

High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome

(2011) S. Alsford et al.   GENOME RESEARCH

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania

(2011) M. B. Rogers et al.   GENOME RESEARCH

Genome-wide RNAi screens in bloodstream form trypanosomes identify drug transporters

(2010) Gabriela Schumann Burkard et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Retention and Loss of RNA Interference Pathways in Trypanosomatid Protozoans

(2010) Lon-Fye Lye et al.   PLoS Pathogens

Regulated expression of the Leishmania major surface virulence factor lipophosphoglycan using conditionally destabilized fusion proteins

(2009) L. Madeira da Silva et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

Improving the photostability of bright monomeric orange and red fluorescent proteins

(2008) Nathan C Shaner et al.   NATURE METHODS