Tracking single molecules at work in living cells

Dynamics of the Serine Chemoreceptor in the Escherichia coli Inner Membrane: A High-Speed Single-Molecule Tracking Study

(2014) Dongmyung Oh et al.   BIOPHYSICAL JOURNAL

Single Molecule Fluorescence Approaches Shed Light on Intracellular RNAs

(2014) Sethuramasundaram Pitchiaya et al.   CHEMICAL REVIEWS

Deciphering the Subunit Composition of Multimeric Proteins by Counting Photobleaching Steps

(2014) Ryan J. Arant et al.   CHEMPHYSCHEM

Objective comparison of particle tracking methods

(2014) Nicolas Chenouard et al.   NATURE METHODS

Precisely and accurately localizing single emitters in fluorescence microscopy

(2014) Hendrik Deschout et al.   NATURE METHODS

Single  -Actin mRNA Detection in Neurons Reveals a Mechanism for Regulating Its Translatability

(2014) A. R. Buxbaum et al.   SCIENCE

Visualization of Dynamics of Single Endogenous mRNA Labeled in Live Mouse

(2014) H. Y. Park et al.   SCIENCE

Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes

(2014) Nao Hiramoto-Yamaki et al.   TRAFFIC

Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae

(2014) Yann Gambin et al.   eLife

Ultra-stable organic fluorophores for single-molecule research

(2013) Qinsi Zheng et al.   CHEMICAL SOCIETY REVIEWS

Single-molecule imaging revealed dynamic GPCR dimerization

(2013) Rinshi S Kasai et al.   CURRENT OPINION IN CELL BIOLOGY

Super-resolution fluorescence imaging with single molecules

(2013) Steffen J Sahl et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Rac1 recruitment to the archipelago structure of the focal adhesion through the fluid membrane as revealed by single-molecule analysis

(2013) Akihiro C. E. Shibata et al.   Cytoskeleton

Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging

(2013) Hirohito Nishimura et al.   JOURNAL OF CELL BIOLOGY

Cutting Edge: Cell Surface Linker for Activation of T Cells Is Recruited to Microclusters and Is Active in Signaling

(2013) L. Balagopalan et al.   JOURNAL OF IMMUNOLOGY

STED microscopy of living cells - new frontiers in membrane and neurobiology

(2013) Christian Eggeling et al.   JOURNAL OF NEUROCHEMISTRY

Spatiotemporal control of endocytosis by phosphatidylinositol-3,4-bisphosphate

(2013) York Posor et al.   NATURE

VAMP7 controls T cell activation by regulating the recruitment and phosphorylation of vesicular Lat at TCR-activation sites

(2013) Paola Larghi et al.   NATURE IMMUNOLOGY

Single-molecule imaging of transcription factor binding to DNA in live mammalian cells

(2013) J Christof M Gebhardt et al.   NATURE METHODS

Caveolae as plasma membrane sensors, protectors and organizers

(2013) Robert G. Parton et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

The Dynamic Synapse

(2013) Daniel Choquet et al.   NEURON

Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy

(2013) Z. W. Zhao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Diversity of Clathrin Function: New Tricks for an Old Protein

(2012) Frances M. Brodsky   Annual Review of Cell and Developmental Biology

Photophysics of Fluorescent Probes for Single-Molecule Biophysics and Super-Resolution Imaging

(2012) Taekjip Ha et al.   Annual Review of Physical Chemistry

Fluorescence correlation spectroscopy

(2012) Jonas Ries et al.   BIOESSAYS

Fluorescence Fluctuation Spectroscopy Enables Quantitative Imaging of Single mRNAs in Living Cells

(2012) Bin Wu et al.   BIOPHYSICAL JOURNAL

The First Five Seconds in the Life of a Clathrin-Coated Pit

(2012) Emanuele Cocucci et al.   CELL

Synaptic structure and function

(2012) Antoine Triller et al.   CURRENT OPINION IN NEUROBIOLOGY

Archipelago architecture of the focal adhesion: Membrane molecules freely enter and exit from the focal adhesion zone

(2012) Akihiro C. E. Shibata et al.   Cytoskeleton

Integrins β1 and β3 exhibit distinct dynamic nanoscale organizations inside focal adhesions

(2012) Olivier Rossier et al.   NATURE CELL BIOLOGY

Transient GPI-anchored protein homodimers are units for raft organization and function

(2012) Kenichi G N Suzuki et al.   Nature Chemical Biology

Conformational states of the kinase Lck regulate clustering in early T cell signaling

(2012) Jérémie Rossy et al.   NATURE IMMUNOLOGY

Single-molecule analysis of gene expression using two-color RNA labeling in live yeast

(2012) Sami Hocine et al.   NATURE METHODS

Confining Domains Lead to Reaction Bursts: Reaction Kinetics in the Plasma Membrane

(2012) Ziya Kalay et al.   PLoS One

Correlation Functions Quantify Super-Resolution Images and Estimate Apparent Clustering Due to Over-Counting

(2012) Sarah L. Veatch et al.   PLoS One

Single-molecule analysis of fluorescently labeled G-protein-coupled receptors reveals complexes with distinct dynamics and organization

(2012) D. Calebiro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Membrane mechanisms for signal transduction: The coupling of the meso-scale raft domains to membrane-skeleton-induced compartments and dynamic protein complexes

(2012) Akihiro Kusumi et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

Regulation from within: the cytoskeleton in transmembrane signaling

(2012) Khuloud Jaqaman et al.   TRENDS IN CELL BIOLOGY

Single-Molecule Imaging of Cell Surfaces Using Near-Field Nanoscopy

(2011) Peter Hinterdorfer et al.   ACCOUNTS OF CHEMICAL RESEARCH

Dynamic Organizing Principles of the Plasma Membrane that Regulate Signal Transduction: Commemorating the Fortieth Anniversary of Singer and Nicolson's Fluid-Mosaic Model

(2011) Akihiro Kusumi et al.   Annual Review of Cell and Developmental Biology

Cytoskeletal Control of CD36 Diffusion Promotes Its Receptor and Signaling Function

(2011) Khuloud Jaqaman et al.   CELL

Regulation of AMPA receptor surface diffusion by PSD-95 slots

(2011) Patricio Opazo et al.   CURRENT OPINION IN NEUROBIOLOGY

Functional Nanoscale Organization of Signaling Molecules Downstream of the T Cell Antigen Receptor

(2011) Eilon Sherman et al.   IMMUNITY

Full characterization of GPCR monomer–dimer dynamic equilibrium by single molecule imaging

(2011) Rinshi S. Kasai et al.   JOURNAL OF CELL BIOLOGY

Central dogma at the single-molecule level in living cells

(2011) Gene-Wei Li et al.   NATURE

Pre-existing clusters of the adaptor Lat do not participate in early T cell signaling events

(2011) David J Williamson et al.   NATURE IMMUNOLOGY

Probing protein heterogeneity in the plasma membrane using PALM and pair correlation analysis

(2011) Prabuddha Sengupta et al.   NATURE METHODS

Fast, three-dimensional super-resolution imaging of live cells

(2011) Sara A Jones et al.   NATURE METHODS

Molecular mechanism and physiological functions of clathrin-mediated endocytosis

(2011) Harvey T. McMahon et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

From the stochasticity of molecular processes to the variability of synaptic transmission

(2011) Claire Ribrault et al.   NATURE REVIEWS NEUROSCIENCE

Super-Resolution Dynamic Imaging of Dendritic Spines Using a Low-Affinity Photoconvertible Actin Probe

(2011) Ignacio Izeddin et al.   PLoS One

T-cell triggering thresholds are modulated by the number of antigen within individual T-cell receptor clusters

(2011) B. N. Manz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Hierarchical mesoscale domain organization of the plasma membrane

(2011) Akihiro Kusumi et al.   TRENDS IN BIOCHEMICAL SCIENCES

Asymmetry of GPCR oligomers supports their functional relevance

(2011) Pascal Maurice et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Membrane Organization and Lipid Rafts

(2011) K. Simons et al.   Cold Spring Harbor Perspectives in Biology

A High Precision Survey of the Molecular Dynamics of Mammalian Clathrin-Mediated Endocytosis

(2011) Marcus J. Taylor et al.   PLOS BIOLOGY

A New Wave of Cellular Imaging

(2010) Derek Toomre et al.   Annual Review of Cell and Developmental Biology

How to obtain labeled proteins and what to do with them

(2010) Marlon J Hinner et al.   CURRENT OPINION IN BIOTECHNOLOGY

Hierarchical organization of the plasma membrane: Investigations by single-molecule tracking vs. fluorescence correlation spectroscopy

(2010) Akihiro Kusumi et al.   FEBS LETTERS

Imaging of Mobile Long-lived Nanoplatforms in the Live Cell Plasma Membrane

(2010) Mario Brameshuber et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Subnanometre single-molecule localization, registration and distance measurements

(2010) Alexandros Pertsinidis et al.   NATURE

Nanoscale architecture of integrin-based cell adhesions

(2010) Pakorn Kanchanawong et al.   NATURE

Spatial control of EGF receptor activation by reversible dimerization on living cells

(2010) Inhee Chung et al.   NATURE

Membrane molecules mobile even after chemical fixation

(2010) Kenji A K Tanaka et al.   NATURE METHODS

Single-Molecule Discrimination of Discrete Perisynaptic and Distributed Sites of Actin Filament Assembly within Dendritic Spines

(2010) Nicholas A. Frost et al.   NEURON

Light Sheet Microscopy for Single Molecule Tracking in Living Tissue

(2010) Jörg Gerhard Ritter et al.   PLoS One

Fast molecular tracking maps nanoscale dynamics of plasma membrane lipids

(2010) S. J. Sahl et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fast live simultaneous multiwavelength four-dimensional optical microscopy

(2010) P. M. Carlton et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Formation and dissociation of M1 muscarinic receptor dimers seen by total internal reflection fluorescence imaging of single molecules

(2010) J. A. Hern et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lipid Rafts As a Membrane-Organizing Principle

(2010) D. Lingwood et al.   SCIENCE

Dynamics of Subsynaptic Vesicles and Surface Microclusters at the Immunological Synapse

(2010) M. A. Purbhoo et al.   Science Signaling

Single-molecule imaging of β-actin mRNAs in the cytoplasm of a living cell

(2009) Mai Yamagishi et al.   EXPERIMENTAL CELL RESEARCH

Small, Mobile FcɛRI Receptor Aggregates Are Signaling Competent

(2009) Nicholas L. Andrews et al.   IMMUNITY

TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation

(2009) Björn F Lillemeier et al.   NATURE IMMUNOLOGY

Quantitative Microscopy: Protein Dynamics and Membrane Organisation

(2009) Dylan M. Owen et al.   TRAFFIC

A single molecule view of gene expression

(2009) Daniel R. Larson et al.   TRENDS IN CELL BIOLOGY

Direct observation of the nanoscale dynamics of membrane lipids in a living cell

(2008) Christian Eggeling et al.   NATURE

Actin restricts FcɛRI diffusion and facilitates antigen-induced receptor immobilization

(2008) Nicholas L. Andrews et al.   NATURE CELL BIOLOGY

Highly inclined thin illumination enables clear single-molecule imaging in cells

(2008) Makio Tokunaga et al.   NATURE METHODS

Robust single-particle tracking in live-cell time-lapse sequences

(2008) Khuloud Jaqaman et al.   NATURE METHODS

High-density mapping of single-molecule trajectories with photoactivated localization microscopy

(2008) Suliana Manley et al.   NATURE METHODS

New Concepts in Synaptic Biology Derived from Single-Molecule Imaging

(2008) Antoine Triller et al.   NEURON