Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM

Modern fluorescent proteins: from chromophore formation to novel intracellular applications

(2012) Olesya Stepanenko et al.   BIOTECHNIQUES

Highly Efficient FRET from a Single Nitrogen-Vacancy Center in Nanodiamonds to a Single Organic Molecule

(2011) Julia Tisler et al.   ACS Nano

Second Harmonic Generation Imaging Microscopy: Applications to Diseases Diagnostics

(2011) Paul Campagnola   ANALYTICAL CHEMISTRY

Development of Probes for Cellular Functions Using Fluorescent Proteins and Fluorescence Resonance Energy Transfer

(2011) Atsushi Miyawaki   Annual Review of Biochemistry

STED Nanoscopy Reveals Molecular Details of Cholesterol- and Cytoskeleton-Modulated Lipid Interactions in Living Cells

(2011) V. Mueller et al.   BIOPHYSICAL JOURNAL

Stable expression of FRET biosensors: A new light in cancer research

(2011) Kazuhiro Aoki et al.   CANCER SCIENCE

Maximum imaging depth of two-photon autofluorescence microscopy in epithelial tissues

(2011) Nicholas J. Durr et al.   JOURNAL OF BIOMEDICAL OPTICS

Quantum Dots in Cell Biology

(2011) Margarida M. Barroso   JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY

Fast scanning STED and two-photon fluorescence excitation microscopy with continuous wave beam

(2011) P. BIANCHINI et al.   JOURNAL OF MICROSCOPY

A modified phasor approach for analyzing time-gated fluorescence lifetime images

(2011) F. FEREIDOUNI et al.   JOURNAL OF MICROSCOPY

Diffraction-unlimited all-optical imaging and writing with a photochromic GFP

(2011) Tim Grotjohann et al.   NATURE

Mechanical strain in actin networks regulates FilGAP and integrin binding to filamin A

(2011) A. J. Ehrlicher et al.   NATURE

A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching

(2011) Tanja Brakemann et al.   NATURE BIOTECHNOLOGY

Two-photon absorption properties of fluorescent proteins

(2011) Mikhail Drobizhev et al.   NATURE METHODS

Imaging protein activity in live embryos using fluorescence resonance energy transfer biosensors

(2011) Elena Kardash et al.   Nature Protocols

Imaging the coordination of multiple signalling activities in living cells

(2011) Christopher M. Welch et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Optical virtual imaging at 50 nm lateral resolution with a white-light nanoscope

(2011) Zengbo Wang et al.   Nature Communications

FRET in membrane biophysics: an overview

(2011) Luís Loura   Frontiers in Physiology

Single-Molecule Four-Color FRET

(2010) Jinwoo Lee  et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Superresolution Imaging using Single-Molecule Localization

(2010) George Patterson et al.   Annual Review of Physical Chemistry

Small photoactivatable molecules for controlled fluorescence activation in living cells

(2010) David Puliti et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Three-Color Spectral FRET Microscopy Localizes Three Interacting Proteins in Living Cells

(2010) Yuansheng Sun et al.   BIOPHYSICAL JOURNAL

The methylated N-terminal tail of RCC1 is required for stabilisation of its interaction with chromatin by Ran in live cells

(2010) Ekarat Hitakomate et al.   BMC CELL BIOLOGY

Nonmuscle Myosin II helps regulate synaptic vesicle mobility at the Drosophila neuromuscular junction

(2010) Sara Seabrooke et al.   BMC NEUROSCIENCE

Fluorescence Lifetime Measurements and Biological Imaging

(2010) Mikhail Y. Berezin et al.   CHEMICAL REVIEWS

Red Fluorescent Protein with Reversibly Photoswitchable Absorbance for Photochromic FRET

(2010) Fedor V. Subach et al.   CHEMISTRY & BIOLOGY

FRET in Cell Biology: Still Shining in the Age of Super-Resolution?

(2010) Hernán E. Grecco et al.   CHEMPHYSCHEM

Nuclear proteins: finding and binding target sites in chromatin

(2010) Martin E. van Royen et al.   CHROMOSOME RESEARCH

FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know?

(2010) Florian Mueller et al.   CURRENT OPINION IN CELL BIOLOGY

Dissecting the binding mechanism of the linker histone in live cells: an integrated FRAP analysis

(2010) Timothy J Stasevich et al.   EMBO JOURNAL

Quantum Dots—From Synthesis to Applications in Biomedicine and Life Sciences

(2010) Gregor Drummen   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Kinectin-mediated endoplasmic reticulum dynamics supports focal adhesion growth in the cellular lamella

(2010) X. Zhang et al.   JOURNAL OF CELL SCIENCE

Live cell imaging of the cancer-related transcription factor RUNX2 during mitotic progression

(2010) Shirwin M. Pockwinse et al.   JOURNAL OF CELLULAR PHYSIOLOGY

FRET for lab-on-a-chip devices — current trends and future prospects

(2010) Smitha S. Varghese et al.   LAB ON A CHIP

Upconversion Nanoparticle-Based FRET System for Study of siRNA in Live Cells

(2010) Shan Jiang et al.   LANGMUIR

Fluorescence lifetime imaging microscopy in life sciences

(2010) Jan Willem Borst et al.   MEASUREMENT SCIENCE and TECHNOLOGY

Biosensors of DsRed as FRET Partner with CFP or GFP for Quantitatively Imaging Induced Activation of Rac, Cdc42 in Living Cells

(2010) Rushi Liu et al.   MOLECULAR IMAGING AND BIOLOGY

Intrinsically Resolution Enhancing Probes for Confocal Microscopy

(2010) Jan Vogelsang et al.   NANO LETTERS

Targeted nanodelivery of drugs and diagnostics

(2010) Margaret A. Phillips et al.   Nano Today

Monitoring multiple distances within a single molecule using switchable FRET

(2010) Stephan Uphoff et al.   NATURE METHODS

Detecting the conformation of individual proteins in live cells

(2010) John J Sakon et al.   NATURE METHODS

Saturated Förster resonance energy transfer microscopy with a stimulated emission depletion beam: a pathway toward single-molecule resolution in far-field bioimaging

(2010) Suhui Deng et al.   OPTICS LETTERS

H-Ras Nanocluster Stability Regulates the Magnitude of MAPK Signal Output

(2010) Barak Rotblat et al.   PLoS One

Small Upconverting Fluorescent Nanoparticles for Biomedical Applications

(2010) Dev K. Chatterjee et al.   Small

GFP: Lighting Up Life (Nobel Lecture)

(2009) Martin Chalfie   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Constructing and Exploiting the Fluorescent Protein Paintbox (Nobel Lecture)

(2009) Roger Y. Tsien   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Discovery of Green Fluorescent Protein (GFP) (Nobel Lecture)

(2009) Osamu Shimomura   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Homo-FRET Imaging Enables Quantification of Protein Cluster Sizes with Subcellular Resolution

(2009) Arjen N. Bader et al.   BIOPHYSICAL JOURNAL

Recent Progress in Strategies for the Creation of Protein-Based Fluorescent Biosensors

(2009) Hangxiang Wang et al.   CHEMBIOCHEM

Lanthanide-Based Luminescent Hybrid Materials

(2009) Koen Binnemans   CHEMICAL REVIEWS

Visualization of molecular interactions using bimolecular fluorescence complementation analysis: Characteristics of protein fragment complementation

(2009) Tom K. Kerppola   CHEMICAL SOCIETY REVIEWS

Genetically encoded biosensors based on engineered fluorescent proteins

(2009) Wolf B. Frommer et al.   CHEMICAL SOCIETY REVIEWS

Reversible Dimerization of EGFR Revealed by Single-Molecule Fluorescence Imaging Using Quantum Dots

(2009) Nagako Kawashima et al.   CHEMISTRY-A EUROPEAN JOURNAL

Photoswitchable Fluorescent Nanoparticles: Preparation, Properties and Applications

(2009) Zhiyuan Tian et al.   CHEMPHYSCHEM

An ultramarine fluorescent protein with increased photostability and pH insensitivity

(2009) Wataru Tomosugi et al.   NATURE METHODS

Photoconversion in orange and red fluorescent proteins

(2009) Gert-Jan Kremers et al.   NATURE METHODS

Super-resolution video microscopy of live cells by structured illumination

(2009) Peter Kner et al.   NATURE METHODS

Two-photon excitation STED microscopy

(2009) Gael Moneron et al.   OPTICS EXPRESS

Anomalous Surplus Energy Transfer Observed with Multiple FRET Acceptors

(2009) Srinagesh V. Koushik et al.   PLoS One

Cholesterol modulates the recruitment of Kv1.5 channels from Rab11-associated recycling endosome in native atrial myocytes

(2009) E. Balse et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Semiconductor quantum dots and metal nanoparticles: syntheses, optical properties, and biological applications

(2008) Vasudevanpillai Biju et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Photon Upconversion in Homogeneous Fluorescence-based Bioanalytical Assays

(2008) Tero Soukka et al.   Annals of the New York Academy of Sciences

Bimolecular Fluorescence Complementation (BiFC) Analysis as a Probe of Protein Interactions in Living Cells

(2008) Tom K. Kerppola   Annual Review of Biophysics

Evidence for a Common Mode of Transcription Factor Interaction with Chromatin as Revealed by Improved Quantitative Fluorescence Recovery after Photobleaching

(2008) Florian Mueller et al.   BIOPHYSICAL JOURNAL

Optical Lock-In Detection of FRET Using Synthetic and Genetically Encoded Optical Switches

(2008) Shu Mao et al.   BIOPHYSICAL JOURNAL

High-precision structural analysis of subnuclear complexes in fixed and live cells via spatially modulated illumination (SMI) microscopy

(2008) Jürgen Reymann et al.   CHROMOSOME RESEARCH

Quantitative analysis of fluorescence lifetime imaging made easy

(2008) Fred S. Wouters et al.   HFSP Journal

Differential dynamics of splicing factor SC35 during the cell cycle

(2008) Kaushlendra Tripathi et al.   JOURNAL OF BIOSCIENCES

Quantification of integrin receptor agonism by fluorescence lifetime imaging

(2008) M. Parsons et al.   JOURNAL OF CELL SCIENCE

Binding of ATP to UAP56 is necessary for mRNA export

(2008) K. P. Kota et al.   JOURNAL OF CELL SCIENCE

Selective detection of luminescence from semiconductor quantum dots by nanosecond time-gated imaging with a colour-masked CCD detector

(2008) A. C. MITCHELL et al.   JOURNAL OF MICROSCOPY

Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors

(2008) Hui-wang Ai et al.   NATURE METHODS

A practical guide to single-molecule FRET

(2008) Rahul Roy et al.   NATURE METHODS

Visualization of protein interactions in living Caenorhabditis elegans using bimolecular fluorescence complementation analysis

(2008) Y John Shyu et al.   Nature Protocols

Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging

(2008) Tom Robinson et al.   OPTICS LETTERS

Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging

(2008) Egidijus Auksorius et al.   OPTICS LETTERS

Quantum dot-based resonance energy transfer and its growing application in biology

(2008) Igor L. Medintz et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

A Molecular Ruler for Measuring Quantitative Distance Distributions

(2008) Rebecca S. Mathew-Fenn et al.   PLoS One

Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy

(2008) B. Huang et al.   SCIENCE

The Phasor Approach to Fluorescence Lifetime Imaging Analysis

(2007) Michelle A. Digman et al.   BIOPHYSICAL JOURNAL

The dynamics of pre-mRNAs and poly(A)+ RNA at speckles in living cells revealed by iFRAP studies

(2007) Yo Ishihama et al.   EXPERIMENTAL CELL RESEARCH