Applications of Phosphate Modification and Labeling to Study (m)RNA Caps

A novel route for preparing 5′ cap mimics and capped RNAs: phosphate-modified cap analogues obtained via click chemistry

(2017) Sylwia Walczak et al.   Chemical Science

Covalent Chemical 5′-Functionalization of RNA with Diazo Reagents

(2016) Christian M. Gampe et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Modifying the 5′-Cap for Click Reactions of Eukaryotic mRNA and To Tune Translation Efficiency in Living Cells

(2016) Josephin M. Holstein et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cytoplasmic RNA decay pathways - Enzymes and mechanisms

(2016) Anna Łabno et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Synthesis of 5′-NAD-Capped RNA

(2016) Katharina Höfer et al.   BIOCONJUGATE CHEMISTRY

Design, synthesis and biological evaluation of dinucleotide mRNA cap analog containing propargyl moiety

(2016) Muthian Shanmugasundaram et al.   BIOORGANIC & MEDICINAL CHEMISTRY

A novel enrichment strategy reveals unprecedented number of novel transcription start sites at single base resolution in a model prokaryote and the gut microbiome

(2016) Laurence Ettwiller et al.   BMC GENOMICS

Cap-like structures in bacterial RNA and epitranscriptomic modification

(2016) Andres Jäschke et al.   CURRENT OPINION IN MICROBIOLOGY

The mechanism of RNA 5′ capping with NAD+, NADH and desphospho-CoA

(2016) Jeremy G. Bird et al.   NATURE

Reversible methylation of m6Am in the 5′ cap controls mRNA stability

(2016) Jan Mauer et al.   NATURE

Structure and function of the bacterial decapping enzyme NudC

(2016) Katharina Höfer et al.   Nature Chemical Biology

Structural basis of mRNA-cap recognition by Dcp1–Dcp2

(2016) Jeffrey S Mugridge et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

mRNA capping: biological functions and applications

(2016) Anand Ramanathan et al.   NUCLEIC ACIDS RESEARCH

Acetylpyrene-labelled 7-methylguanine nucleotides: unusual fluorescence properties and application to decapping scavenger activity monitoring

(2016) Renata Kasprzyk et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

A fluorescent HTS assay for phosphohydrolases based on nucleoside 5′-fluorophosphates: its application in screening for inhibitors of mRNA decapping scavenger and PDE-I

(2016) M. R. Baranowski et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Structural basis for m7G recognition and 2′-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I

(2016) Swapnil C. Devarkar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Two-headed tetraphosphate cap analogs are inhibitors of the Dcp1/2 RNA decapping complex

(2016) Marcin Ziemniak et al.   RNA

Structural basis of mRNA-cap recognition by Dcp1–Dcp2

(2016) Jeffrey S Mugridge et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Clickable trimethylguanosine cap analogs modified within the triphosphate bridge: synthesis, conjugation to RNA and susceptibility to degradation

(2016) Blazej A. Wojtczak et al.   RSC Advances

Synthetic m3G-CAP attachment necessitates a minimum trinucleotide constituent to be recognised as a nuclear import signal

(2016) M. Honcharenko et al.   RSC Advances

Phosphate-modified analogues of m 7 GTP and m 7 Gppppm 7 G—Synthesis and biochemical properties

(2015) Marcin Ziemniak et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Synthesis of Fluorophosphate Nucleotide Analogues and Their Characterization as Tools for 19F NMR Studies

(2015) Marek R. Baranowski et al.   JOURNAL OF ORGANIC CHEMISTRY

Synthetic mRNAs for manipulating cellular phenotypes: an overview

(2015) Elgar Susanne Quabius et al.   New Biotechnology

Discovery of m7G-cap in eukaryotic mRNAs

(2015) Yasuhiro FURUICHI   PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES

RNA capping: progress and prospects

(2015) Stewart Shuman   RNA

NAD in RNA: unconventional headgear

(2015) Daniel J. Luciano et al.   TRENDS IN BIOCHEMICAL SCIENCES

Bioorthogonal site-specific labeling of the 5′-cap structure in eukaryotic mRNAs

(2014) Josephin Marie Holstein et al.   CHEMICAL COMMUNICATIONS

Elimination of cap structures generated by mRNA decay involves the new scavenger mRNA decapping enzyme Aph1/FHIT together with DcpS

(2014) Valerio Taverniti et al.   NUCLEIC ACIDS RESEARCH

Hypermethylated-capped selenoprotein mRNAs in mammals

(2014) Laurence Wurth et al.   NUCLEIC ACIDS RESEARCH

Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes

(2014) Joanna Kowalska et al.   NUCLEIC ACIDS RESEARCH

Synthesis of Capped RNA using a DMT Group as a Purification Handle

(2014) Elizabeth Veliath et al.   NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS

Towards novel efficient and stable nuclear import signals: synthesis and properties of trimethylguanosine cap analogs modified within the 5′,5′-triphosphate bridge

(2014) Malgorzata Zytek et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Crystal Structures of the Novel Cytosolic 5′-Nucleotidase IIIB Explain Its Preference for m7GMP

(2014) Thomas Monecke et al.   PLoS One

A Chemo-Enzymatic Approach for Site-Specific Modification of the RNA Cap

(2013) Daniela Schulz et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The synthesis of isopropylidene mRNA cap analogs modified with phosphorothioate moiety and their evaluation as promoters of mRNA translation

(2013) Marcin Warminski et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Potential therapeutic applications of RNA cap analogs

(2013) Marcin Ziemniak et al.   Future Medicinal Chemistry

Chemical Synthesis of U1 snRNA Derivatives

(2013) Akihiro Ohkubo et al.   ORGANIC LETTERS

Enzymatic Synthesis of RNAs Capped with Nucleotide Analogues Reveals the Molecular Basis for Substrate Selectivity of RNA Capping Enzyme: Impacts on RNA Metabolism

(2013) Moheshwarnath Issur et al.   PLoS One

Multiple Nudix family proteins possess mRNA decapping activity

(2013) M.-G. Song et al.   RNA

Synthesis and evaluation of fluorescent cap analogues for mRNA labelling

(2013) Marcin Ziemniak et al.   RSC Advances

7-Methylguanosine Diphosphate (m7GDP) Is Not Hydrolyzed but Strongly Bound by Decapping Scavenger (DcpS) Enzymes and Potently Inhibits Their Activity

(2012) Anna Wypijewska et al.   BIOCHEMISTRY

Structural and functional control of the eukaryotic mRNA decapping machinery

(2012) Marcos Arribas-Layton et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

Synthesis and properties of mRNA cap analogs containing imidodiphosphate moiety—fairly mimicking natural cap structure, yet resistant to enzymatic hydrolysis

(2012) Anna M. Rydzik et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Synthesis of nucleoside phosphosulfates

(2012) Joanna Kowalska et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Identification ofDrosophilaand Human 7-Methyl GMP-specific Nucleotidases

(2012) Juliane Buschmann et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure-Guided Design, Synthesis, and Evaluation of Guanine-Derived Inhibitors of the eIF4E mRNA–Cap Interaction

(2012) Xiaoqi Chen et al.   JOURNAL OF MEDICINAL CHEMISTRY

Synthesis of biotin labelled cap analogue – incorporable into mRNA transcripts and promoting cap-dependent translation

(2012) Jacek Jemielity et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Preparation of Synthetically Challenging Nucleotides Using Cyanoethyl P-Imidazolides and Microwaves

(2012) Malwina Strenkowska et al.   ORGANIC LETTERS

Synthesis of 5' cap-0 and cap-1 RNAs using solid-phase chemistry coupled with enzymatic methylation by human (guanine-N7)-methyl transferase

(2012) Y. Thillier et al.   RNA

5′-Terminal chemical capping of spliced leader RNAs

(2012) Karolina Piecyk et al.   TETRAHEDRON LETTERS

Photoresponsive 5′-cap for the reversible photoregulation of gene expression

(2011) Shinzi Ogasawara et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Conventional and unconventional mechanisms for capping viral mRNA

(2011) Etienne Decroly et al.   NATURE REVIEWS MICROBIOLOGY

Translation, stability, and resistance to decapping of mRNAs containing caps substituted in the triphosphate chain with BH3, Se, and NH

(2011) W. Su et al.   RNA

Synthesis and evaluation of 2′-O-allyl substituted dinucleotide cap analog for mRNA translation

(2010) Anilkumar R. Kore et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Phosphorothioate cap analogs increase stability and translational efficiency of RNA vaccines in immature dendritic cells and induce superior immune responses in vivo

(2010) A N Kuhn et al.   GENE THERAPY

Multiple mRNA Decapping Enzymes in Mammalian Cells

(2010) Man-Gen Song et al.   MOLECULAR CELL

2′-O methylation of the viral mRNA cap evades host restriction by IFIT family members

(2010) Stephane Daffis et al.   NATURE

Synthetic mRNA cap analogs with a modified triphosphate bridge – synthesis, applications and prospects

(2010) Jacek Jemielity et al.   NEW JOURNAL OF CHEMISTRY

Cap and cap-binding proteins in the control of gene expression

(2010) Ivan Topisirovic et al.   Wiley Interdisciplinary Reviews-RNA

Efficient solid-phase synthesis of oligodeoxynucleotides having a 5′-terminal 2,2,7-trimethylguanosine pyrophosphate linkage

(2009) Akihiro Ohkubo et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Phosphoroselenoate Dinucleotides for Modification of mRNA 5′ End

(2009) Joanna Kowalska et al.   CHEMBIOCHEM

Synthesis and Application of 2′-ara-Fluoroguanosine-substituted Cap Analog

(2009) Anilkumar R. Kore et al.   CHEMISTRY LETTERS

Locked Nucleic Acid (LNA)-Modified Dinucleotide mRNA Cap Analogue: Synthesis, Enzymatic Incorporation, and Utilization

(2009) Anilkumar R. Kore et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

LC/MS analysis of cellular RNA reveals NAD-linked RNA

(2009) Y Grace Chen et al.   Nature Chemical Biology

Structural basis for m7G-cap hypermethylation of small nuclear, small nucleolar and telomerase RNA by the dimethyltransferase TGS1

(2009) Thomas Monecke et al.   NUCLEIC ACIDS RESEARCH

Synthetic dinucleotide mRNA cap analogs with tetraphosphate 5′,5′ bridge containing methylenebis(phosphonate) modification

(2009) Anna Maria Rydzik et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Synthesis and characterization of mRNA cap analogs containing phosphorothioate substitutions that bind tightly to eIF4E and are resistant to the decapping pyrophosphatase DcpS

(2008) J. Kowalska et al.   RNA