Enhancing Stability of Camelid and Shark Single Domain Antibodies: An Overview

Improved production of single domain antibodies with two disulfide bonds by co-expression of chaperone proteins in the Escherichia coli periplasm

(2017) Lisa C. Shriver-Lake et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Evaluation of anti-botulinum neurotoxin single domain antibodies with additional optimization for improved production and stability

(2017) Lisa C. Shriver-Lake et al.   TOXICON

Thermal stabilization of anti-α-cobratoxin single domain antibodies

(2017) George P. Anderson et al.   TOXICON

Stability of isolated antibody-antigen complexes as a predictive tool for selecting toxin neutralizing antibodies

(2016) Patricia M. Legler et al.   mAbs

Fusion of the mouse IgG1 Fc domain to the VHH fragment (ARP1) enhances protection in a mouse model of rotavirus

(2016) Gökçe Günaydın et al.   Scientific Reports

Importance of Hypervariable Region 2 for Stability and Affinity of a Shark Single-Domain Antibody Specific for Ebola Virus Nucleoprotein

(2016) George P. Anderson et al.   PLoS One

ANARCI: antigen receptor numbering and receptor classification

(2015) James Dunbar et al.   BIOINFORMATICS

The role of intra-domain disulfide bonds in heat-induced irreversible denaturation of camelid single domain VHH antibodies

(2015) Yoko Akazawa-Ogawa et al.   JOURNAL OF BIOCHEMISTRY

Accurate secondary structure prediction and fold recognition for circular dichroism spectroscopy

(2015) András Micsonai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering disulfide bonds within an antibody

(2014) Yoshihisa Hagihara et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Mutational approaches to improve the biophysical properties of human single-domain antibodies

(2014) Dae Young Kim et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Heat-induced Irreversible Denaturation of the Camelid Single Domain VHH Antibody Is Governed by Chemical Modifications

(2014) Yoko Akazawa-Ogawa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Bioconjugates of rhizavidin with single domain antibodies as bifunctional immunoreagents

(2014) Jinny L. Liu et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Structural insights and biomedical potential of IgNAR scaffolds from sharks

(2014) Stefan Zielonka et al.   mAbs

Thermal stability and refolding capability of shark derived single domain antibodies

(2014) Jinny L. Liu et al.   MOLECULAR IMMUNOLOGY

Evaluation of Disulfide Bond Position to Enhance the Thermal Stability of a Highly Stable Single Domain Antibody

(2014) Dan Zabetakis et al.   PLoS One

Enhanced stabilization of a stable single domain antibody for SEB toxin by random mutagenesis and stringent selection

(2014) K. B. Turner et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Optimal charged mutations in the complementarity-determining regions that prevent domain antibody aggregation are dependent on the antibody scaffold

(2014) J. M. Perchiacca et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Protease-resistant single-domain antibodies inhibit Campylobacter jejuni motility

(2014) G. Hussack et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Negative tail fusions can improve ruggedness of single domain antibodies

(2014) Ellen R. Goldman et al.   PROTEIN EXPRESSION AND PURIFICATION

Structural and mutational analysis of a monomeric and dimeric form of a single domain antibody with implications for protein misfolding

(2014) Jade George et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Thermostable single domain antibody–maltose binding protein fusion for Bacillus anthracis spore protein BclA detection

(2013) Scott A. Walper et al.   ANALYTICAL BIOCHEMISTRY

Stability engineering of the human antibody repertoire

(2013) Romain Rouet et al.   FEBS LETTERS

Atypical Antigen Recognition Mode of a Shark Immunoglobulin New Antigen Receptor (IgNAR) Variable Domain Characterized by Humanization and Structural Analysis

(2013) Oleg V. Kovalenko et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Single-Domain Antibody-Based and Linker-Free Bispecific Antibodies Targeting Fc RIII Induce Potent Antitumor Activity without Recruiting Regulatory T Cells

(2013) C. Rozan et al.   MOLECULAR CANCER THERAPEUTICS

Contributions of the Complementarity Determining Regions to the Thermal Stability of a Single-Domain Antibody

(2013) Dan Zabetakis et al.   PLoS One

Differential scanning calorimetry as a tool for protein folding and stability

(2012) Christopher M. Johnson   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Comparison of single domain antibody immobilization strategies evaluated by surface plasmon resonance

(2012) Scott A. Walper et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Improving the pharmacokinetic properties of biologics by fusion to an anti-HSA shark VNAR domain

(2012) Mischa R. Müller et al.   mAbs

An integrated approach to extreme thermostabilization and affinity maturation of an antibody

(2012) A. D. McConnell et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Aggregation-resistant domain antibodies engineered with charged mutations near the edges of the complementarity-determining regions

(2012) J. M. Perchiacca et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Immunodiagnostic reagents using llama single domain antibody–alkaline phosphatase fusion proteins

(2011) Marla D. Swain et al.   ANALYTICAL BIOCHEMISTRY

Isolation of a Highly Thermal Stable Lama Single Domain Antibody Specific for Staphylococcus aureus Enterotoxin B

(2011) Russell R Graef et al.   BMC BIOTECHNOLOGY

Biotechnological applications of recombinant single-domain antibody fragments

(2011) Ario de Marco   Microbial Cell Factories

Isolation and characterisation of Ebolavirus-specific recombinant antibody fragments from murine and shark immune libraries

(2011) Sarah A. Goodchild et al.   MOLECULAR IMMUNOLOGY

Engineered Single-Domain Antibodies with High Protease Resistance and Thermal Stability

(2011) Greg Hussack et al.   PLoS One

Binding Kinetics of Antiricin Single Domain Antibodies and Improved Detection Using a B Chain Specific Binder

(2010) George P. Anderson et al.   ANALYTICAL CHEMISTRY

Single-domain llama antibodies as specific intracellular inhibitors of SpvB, the actin ADP-ribosylating toxin of Salmonella typhimurium

(2010) Vanina Alzogaray et al.   FASEB JOURNAL

High-Throughput Thermal Scanning: A General, Rapid Dye-Binding Thermal Shift Screen for Protein Engineering

(2009) Jason J. Lavinder et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Single domain antibodies: promising experimental and therapeutic tools in infection and immunity

(2009) Janusz Wesolowski et al.   MEDICAL MICROBIOLOGY AND IMMUNOLOGY

Engineering a Camelid Antibody Fragment That Binds to the Active Site of Human Lysozyme and Inhibits Its Conversion into Amyloid Fibrils†

(2008) Pak-Ho Chan et al.   BIOCHEMISTRY

General Strategy to Humanize a Camelid Single-domain Antibody and Identification of a Universal Humanized Nanobody Scaffold

(2008) Cécile Vincke et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Disulfide Bond Introduction for General Stabilization of Immunoglobulin Heavy-Chain Variable Domains

(2008) Dirk Saerens et al.   JOURNAL OF MOLECULAR BIOLOGY

Aggregation-resistant VHs selected by in vitro evolution tend to have disulfide-bonded loops and acidic isoelectric points

(2008) M. Arbabi-Ghahroudi et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Camelid immunoglobulins and nanobody technology

(2008) S. Muyldermans et al.   VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

Single-domain antibody fragments with high conformational stability

(2002) Mireille Dumoulin et al.   PROTEIN SCIENCE