Review
Cell Biology
Jana Petrusova, Jasper Manning, Dominik Filipp
Summary: Actin is a multi-functional protein involved in various cellular processes. Its role in cytoskeleton regulation, cell migration, and cellular integrity has been well-studied. Recently, it has been found that actin also plays a role in the nucleus, participating in transcription, chromatin remodeling, and DNA damage repair. In the context of germ cell division, actin may be involved in meiotic prophase I and its interaction with chromatin remodeling complexes to control the pairing and recombination of homologous chromosomes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Plant Sciences
Gilles Dupouy, Yihan Dong, Etienne Herzog, Marie-Edith Chaboute, Alexandre Berr
Summary: The nucleus is a central organelle in eukaryotic cells that undergoes dynamic structural changes during cellular processes. This review focuses on recent data obtained in plants, particularly in response to mechanical stress, and highlights the similarities and differences between animal and plant cells in terms of nuclear envelope dynamics and functional impacts.
Article
Plant Sciences
Joseph F. McKenna, Hardeep K. Gumber, Zachary M. Turpin, Alexis M. Jalovec, Andre C. Kartick, Katja Graumann, Hank W. Bass
Summary: The study demonstrates that nucleoskeletal proteins NCH1, NCH2, and MKAKU41 in maize have characteristic properties of LINC-associated plant nucleoskeletal proteins, impacting the nuclear periphery structure and overall nuclear architecture.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Apekshya Chhetri, Joseph V. Rispoli, Sophie A. Lelievre
Summary: Discoveries about the changing stiffness of the tumor microenvironment during cancer progression have led to the development of cell culture methods involving extracellular mechanostimuli to understand mechanotransduction mechanisms impacting cell phenotypes. Collagen I is commonly used in 3D cell culture to study mechanotransduction, while models with interstitial fluid stress focus on invasive cell migration. Integrating increased ECM stiffness and fluid flow in tumor culture is crucial, as is the need for new techniques to study the physical plasticity of cancer cells in 3D. Investigating the physical heterogeneity of the TME and its impact on cellular heterogeneity could enhance collaborative efforts between biologists and engineers to advance mechanomedicine in oncology.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Bruno Monier, Magali Suzanne
Summary: Apoptosis, a crucial form of cell suicide, plays important roles in eliminating unnecessary cells, protecting the body against harmful cells, and promoting tissue morphogenesis during animal development. This process involves activation of dedicated enzymes called caspases, as well as mechanical forces and nuclear coupling for nucleus destruction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Yuqi Tian, Libo Liu, Jinmin Gao, Ruoxi Wang
Summary: Meiosis is a specialized cell division that generates haploid gametes in sexually reproducing organisms. Homologous chromosomes pair and segregate, but the mechanisms of pairing differ across species. Recent studies have identified conserved mechanisms and key regulators of homologous chromosome pairing in various model organisms.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Cell Biology
William Bachman, Rupalatha Maddala, Ayon Chakraborty, Camelia Eldawy, Nikolai P. Skiba, Ponugoti Rao
Summary: Clinical use of glucocorticoids is associated with increased intraocular pressure, a major risk factor for glaucoma. Glucocorticoids have been found to induce changes in actin cytoskeletal organization, cell adhesion, extracellular matrix, and other cellular activities in trabecular meshwork (TM) cells, which play a crucial role in aqueous humor dynamics and intraocular pressure regulation. However, there is limited understanding of the molecular mechanisms underlying these changes in TM cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Scott Birks, Gunes Uzer
Summary: The nuclear envelope and nucleoskeleton play a key role in transducing physical information into the nucleus, affecting chromatin and controlling cell function. Diseases related to nuclear envelope protein mutations, such as Emery-Dreifuss muscular dystrophies and progeria, are associated with musculoskeletal and cardiac symptoms. The impact of nuclear envelope mechanobiology on bone function remains understudied, with mesenchymal stem cells being a key focus in understanding the relationship between bone regulation and nuclear envelope function.
Article
Biochemical Research Methods
Rahul Sureka, Rakesh Mishra
Summary: By studying the developing embryos of Drosophila melanogaster and Danio rerio, 362 core NuMat proteins conserved between the two organisms were identified. Comparisons with publicly available Mus musculus NuMat dataset and Homo sapiens cellular localization dataset revealed 252 core homologous NuMat proteins, with 86 protein groups originating from preexisting proteins in prokaryotes. Thirty-six were conserved across all eukaryotic supergroups, 14 evolved before the last eukaryotic common ancestor, and these 50 proteins out of the 252 core conserved NuMat proteins have been conserved across all eukaryotes for over 1.5 billion years, indicating their crucial role in nuclear function.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Cell Biology
Audrey Yi Tyan Peng, Janhavi A. Kolhe, Lindsey D. Behrens, Brian C. Freeman
Summary: The text discusses the cellular mechanisms dictating chromatin movements, including the roles of epigenetic modifications, transcription factors, and the nucleoskeleton system in chromosome folding and positioning.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Hongxin Wang, Han Zhang, Ryo Tamura, Bo Da, Shimaa A. Abdellatef, Ikumu Watanabe, Nobuyuki Ishida, Daisuke Fujita, Nobutaka Hanagata, Tomoki Nakagawa, Jun Nakanishi
Summary: This study investigates the cellular response to environmental stimuli and the correlation between intracellular force distribution and cytoskeletal structures. The results show that dynamic forces within cells play a crucial role in adaptive survival, cancer growth, and cell migration.
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
(2023)
Review
Physiology
Bo Zhang, Joseph D. Powers, Andrew D. McCulloch, Neil C. Chi
Summary: Mechanosignaling is the process of converting biomechanical stimuli into cellular responses. External forces are transmitted through protein networks from the cell membrane to the cytoskeleton and nucleus, regulating gene expression and cell remodeling. This review discusses recent experimental work on the pathway of biomechanical stimulus propagation in striated muscles, highlighting its role in gene regulation, muscle structure, and function. It also addresses nuclear protein mutations that affect mechanosignaling in cardiomyopathy models and the open questions and future challenges in studying striated muscle nuclear mechanosignaling.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Pragati C. Marks, Ryan J. Petrie
Summary: The movement of the nucleus is a crucial step in 3D cell migration, requiring coordination between the cytoskeletal and nucleoskeletal networks. Recent studies have identified unique molecular crosstalk that can drive nuclear migration in different 3D environments, as well as the potential role of proteins that indirectly crosslink cytoskeletal networks and 3D focal adhesions in driving 3D nuclear migration.
Editorial Material
Biochemistry & Molecular Biology
Kei Miyamoto, Masahiko Harata
Summary: The eukaryotic nucleus not only contains chromosomes and transcriptional components, but also structural filaments like nucleoskeleton proteins that support nuclear organization and compartments. These nucleoskeleton proteins, such as lamins and actin, influence nuclear dynamics including transcriptional regulation, chromatin organization, and DNA damage responses, playing crucial roles in cellular differentiation and animal development.
JOURNAL OF BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Matthew Goelzer, Amel Dudakovic, Melis Olcum, Buer Sen, Engin Ozcivici, Janet Rubin, Andre J. van Wijnen, Gunes Uzer
Summary: Mesenchymal stem cells (MSCs) maintain musculoskeletal system by differentiating into osteoblasts and adipocytes. Mechanical signals like strain and low-intensity vibration regulate MSC differentiation via cell structure control. Lamin A/C protein is crucial for nuclear architecture, and its depletion affects adipogenesis markers and cellular stiffness. The study suggests mechanoregulation of adipogenesis and mechanical activation of focal adhesions happen independently from lamin A/C.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Takeshi Shimi, Mark Kittisopikul, Joseph Tran, Anne E. Goldman, Stephen A. Adam, Yixian Zheng, Khuloud Jaqaman, Robert D. Goldman
MOLECULAR BIOLOGY OF THE CELL
(2015)
Article
Multidisciplinary Sciences
Zhixun Dou, Caiyue Xu, Greg Donahue, Takeshi Shimi, Ji-An Pan, Jiajun Zhu, Andrejs Ivanov, Brian C. Capelll, Adam M. Drake, Parisha P. Shah, Joseph M. Catanzaro, M. Daniel Ricketts, Trond Lamark, Stephen A. Adam, Ronen Marmorstein, Wei-Xing Zong, Terje Johansen, Robert D. Goldman, Peter D. Adams, Shelley L. Berger
Article
Multidisciplinary Sciences
Amelie Robert, Molly J. Rossow, Caroline Hookway, Stephen A. Adam, Vladimir I. Gelfand
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Cell Biology
Andrew D. Stephens, Edward J. Banigan, Stephen A. Adam, Robert D. Goldman, John F. Marko
MOLECULAR BIOLOGY OF THE CELL
(2017)
Article
Biochemistry & Molecular Biology
Amelie Robert, Peirun Tian, Stephen A. Adam, Mark Kittisopikul, Khuloud Jaqaman, Robert D. Goldman, Vladimir I. Gelfand
Article
Cell Biology
Andrew D. Stephens, Patrick Z. Liu, Edward J. Banigan, Luay M. Almassalha, Vadim Backman, Stephen A. Adam, Robert D. Goldman, John F. Marko
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Cell Biology
Yu Chen, Yang Zhang, Yuchuan Wang, Liguo Zhang, Eva K. Brinkman, Stephen A. Adams, Robert Goldman, Bas van Steensel, Jian Ma, Andrew S. Belmont
JOURNAL OF CELL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Veronika Butin-Israeli, Stephen A. Adam, Nikhil Jain, Gabriel L. Otte, Daniel Neems, Lisa Wiesmueller, Shelly L. Berger, Robert D. Goldman
MOLECULAR AND CELLULAR BIOLOGY
(2015)
Article
Medicine, Research & Experimental
Veronika Butin-Israeli, Triet M. Bui, Hannah L. Wiesolek, Lorraine Mascarenhas, Joseph J. Lee, Lindsey C. Mehl, Kaitlyn R. Knutson, Stephen A. Adam, Robert D. Goldman, Arthur Beyder, Lisa Wiesmuller, Stephen B. Hanauer, Ronen Sumagin
JOURNAL OF CLINICAL INVESTIGATION
(2019)
Article
Cell Biology
Alison E. Patteson, Amir Vahabikashi, Katarzyna Pogoda, Stephen A. Adam, Kalpana Mandal, Mark Kittisopikul, Suganya Sivagurunathan, Anne Goldman, Robert D. Goldman, Paul A. Janmey
JOURNAL OF CELL BIOLOGY
(2019)
Article
Cell Biology
Joseph R. Tran, Danielle I. Paulson, James J. Moresco, Stephen A. Adam, John R. Yates, Robert D. Goldman, Yixian Zheng
Summary: The study successfully identified proteins, RNA, and DNA associated with or proximal to the nuclear lamina by fusing APEX2 with the nuclear lamina protein lamin-B1, revealing important structural and functional characteristics.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Mark Kittisopikul, Takeshi Shimi, Meltem Tatli, Joseph Riley Tran, Yixian Zheng, Ohad Medalia, Khuloud Jaqaman, Stephen A. Adam, Robert D. Goldman
Summary: The study demonstrates that lamin isoforms and nucleoporins work together to maintain the normal organization of lamin meshworks and NPC within the nuclear envelope; Specific lamin isoforms are involved in regulating the number of NPCs;
The spatial association between lamin isoform fibers and NPCs remains intact in nuclei with knockout lamin isoforms.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Huayin Wu, Yinan Shen, Suganya Sivagurunathan, Miriam Sarah Weber, Stephen A. Adam, Jennifer H. Shin, Jeffrey J. Fredberg, Ohad Medalia, Robert Goldman, David A. Weitz
Summary: The cytoskeleton of eukaryotic cells consists of various filamentous proteins, including F-actin, microtubules, and intermediate filaments. Interactions between these cytoskeletal components play a crucial role in determining cell structure and function. This study investigates the interplay between F-actin and vimentin intermediate filament (VIF) networks, revealing their synergistic effects in both structure and function. These findings significantly broaden our understanding of the contributions of the cytoskeleton components, particularly the interactions between intermediate filaments and F-actin.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Yohei Kono, Stephen A. Adam, Yuko Sato, Karen L. Reddy, Yixian Zheng, Ohad Medalia, Robert D. Goldman, Hiroshi Kimura, Takeshi Shimi
Summary: The major structural component of nuclear lamina, lamin C, rapidly accumulates at sites of NE rupture, promoting repair. This accumulation is dependent on the immunoglobulin-like fold domain that binds to BAF and a nuclear localization signal, and is partly responsible for the accumulation of nuclear BAF and cytoplasmic cGAS.
JOURNAL OF CELL BIOLOGY
(2022)