A novel technique based on in vitro oocyte injection to improve CRISPR/Cas9 gene editing in zebrafish

Utilization of the CRISPR/Cas9 system for the efficient production of mutant mice using crRNA/tracrRNA with Cas9 nickase and FokI-dCas9

(2016) Miho Terao et al.   EXPERIMENTAL ANIMALS

Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus

(2016) Chi Zhang et al.   FUNGAL GENETICS AND BIOLOGY

Closed oocyte vitrification and storage in an oocyte donation programme: obstetric and neonatal outcome

(2016) N. De Munck et al.   HUMAN REPRODUCTION

The effect of temperature during liquid storage of in vitro–matured bovine oocytes on subsequent embryo development

(2016) Tayita Suttirojpattana et al.   THERIOGENOLOGY

Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 °C

(2016) A. Ubilla et al.   THERIOGENOLOGY

Synthetic RNA Polymerase III Promoters Facilitate High-Efficiency CRISPR–Cas9-Mediated Genome Editing in Yarrowia lipolytica

(2016) Cory M. Schwartz et al.   ACS Synthetic Biology

Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases

(2016) Jean-Baptiste Renaud et al.   Cell Reports

Construction of a CRISPR-Cas9 System for Pig Genome Targeting

(2015) Yu-Hsiu Su et al.   ANIMAL BIOTECHNOLOGY

Effect of magnetized extender on sperm membrane integrity and development of oocytes in vitro fertilized with liquid storage boar semen

(2015) Sang-Hee Lee et al.   ANIMAL REPRODUCTION SCIENCE

CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning

(2015) Jia-Wang Wang et al.   BIOTECHNIQUES

Six years' experience in ovum donation using vitrified oocytes: report of cumulative outcomes, impact of storage time, and development of a predictive model for oocyte survival rate

(2015) Ana Cobo et al.   FERTILITY AND STERILITY

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9

(2015) Gaurav K. Varshney et al.   GENOME RESEARCH

High-Efficiency Genome Editing in Arabidopsis Using YAO Promoter-Driven CRISPR/Cas9 System

(2015) Liuhua Yan et al.   Molecular Plant

Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

(2015) Takeshi Maruyama et al.   NATURE BIOTECHNOLOGY

CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity

(2015) Blake Carrington et al.   NUCLEIC ACIDS RESEARCH

Generation of gene-target dogs using CRISPR/Cas9 system

(2015) Qingjian Zou et al.   Journal of Molecular Cell Biology

Highly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevis

(2015) K. Nakajima et al.   Biology Open

Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish

(2015) Hirohito Kotani et al.   PLoS One

Improvement of gamete quality and its short-term storage: an approach for biotechnology in laboratory fish

(2014) G. S. Yasui et al.   Animal

Storage of Steindachneridion parahybae oocytes at different temperatures

(2014) Eduardo Antônio Sanches et al.   ANIMAL REPRODUCTION SCIENCE

Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos

(2014) Yuyu Niu et al.   CELL

Improving the efficiency for generation of genome-edited zebrafish by labeling primordial germ cells

(2014) Zhangji Dong et al.   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

Successful live birth from oocytes after more than 14 years of cryopreservation

(2014) María Fernanda Urquiza et al.   JOURNAL OF ASSISTED REPRODUCTION AND GENETICS

Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs

(2014) James A. Gagnon et al.   PLoS One

A robust TALENs system for highly efficient mammalian genome editing

(2014) Yuanxi Feng et al.   Scientific Reports

Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering

(2014) Yukiko Kimura et al.   Scientific Reports

RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein

(2014) Jeong-Soo Lee et al.   G3-Genes Genomes Genetics

Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos

(2013) Nannan Chang et al.   CELL RESEARCH

Efficient CRISPR/Cas9 genome editing with low off-target effects in zebrafish

(2013) A. Hruscha et al.   DEVELOPMENT

Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases

(2013) Y. H. Sung et al.   GENOME RESEARCH

Quantitative Data Analysis with IBM SPSS 17, 18 & 19: A Guide for Social Scientists by Alan Bryman and Duncan Cramer

(2013) Susan Starkings   INTERNATIONAL STATISTICAL REVIEW

Efficient genome editing in zebrafish using a CRISPR-Cas system

(2013) Woong Y Hwang et al.   NATURE BIOTECHNOLOGY

Heritable genome editing in C. elegans via a CRISPR-Cas9 system

(2013) Ari E Friedland et al.   NATURE METHODS

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System

(2013) Woong Y. Hwang et al.   PLoS One

Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

(2013) Li-En Jao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

In vitro oocyte culture-based manipulation of zebrafish maternal genes

(2012) Sreelaja Nair et al.   DEVELOPMENTAL DYNAMICS

In vivo genome editing using a high-efficiency TALEN system

(2012) Victoria M. Bedell et al.   NATURE

High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases

(2011) Fuqiang Chen et al.   NATURE METHODS

CTAB methods for DNA extraction of sweetpotato for microsatellite analysis

(2009) Aline Borges et al.   SCIENTIA AGRICOLA

Zinc-finger nucleases: a powerful tool for genetic engineering of animals

(2009) Séverine Rémy et al.   TRANSGENIC RESEARCH

Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases

(2008) Yannick Doyon et al.   NATURE BIOTECHNOLOGY

Development of a reliable in vitro maturation system for zebrafish oocytes

(2008) Shinsuke Seki et al.   REPRODUCTION