Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Targeting hepatitis B virus cccDNA using CRISPR/Cas9

(2015) Edward M. Kennedy et al.   ANTIVIRAL RESEARCH

Targeting hepatitis B virus cccDNA by CRISPR/Cas9 nuclease efficiently inhibits viral replication

(2015) Chunsheng Dong et al.   ANTIVIRAL RESEARCH

Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes

(2015) Yoshiko Nakagawa et al.   BMC BIOTECHNOLOGY

Crystal Structure of Staphylococcus aureus Cas9

(2015) Hiroshi Nishimasu et al.   CELL

How specific is CRISPR/Cas9 really?

(2015) Henriette O’Geen et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Application of CRISPR/Cas9 Technology to HBV

(2015) Guigao Lin et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Estimations of worldwide prevalence of chronic hepatitis B virus infection: a systematic review of data published between 1965 and 2013

(2015) Aparna Schweitzer et al.   LANCET

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Dual gRNAs guided CRISPR/Cas9 system inhibits hepatitis B virus replication

(2015) Jie Wang   WORLD JOURNAL OF GASTROENTEROLOGY

CRISPR/Cas9 nickase-mediated disruption of hepatitis B virus open reading frame S and X

(2015) Madina Karimova et al.   Scientific Reports

CRISPR/Cas9-based tools for targeted genome editing and replication control of HBV

(2015) Cheng Peng et al.   VIROLOGICA SINICA

Nuclease-mediated genome editing: At the front-line of functional genomics technology

(2014) Tetsushi Sakuma et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification

(2014) John P Guilinger et al.   NATURE BIOTECHNOLOGY

Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

(2014) Tetsushi Sakuma et al.   Scientific Reports

The CRISPR/Cas9 System Facilitates Clearance of the Intrahepatic HBV Templates In Vivo

(2014) Su-Ru Lin et al.   Molecular Therapy-Nucleic Acids

COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites

(2014) Thomas J Cradick et al.   Molecular Therapy-Nucleic Acids

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Efficient TALEN construction and evaluation methods for human cell and animal applications

(2013) Tetsushi Sakuma et al.   GENES TO CELLS

Inactivation of Hepatitis B Virus Replication in Cultured Cells and In Vivo with Engineered Transcription Activator-Like Effector Nucleases

(2013) Kristie Bloom et al.   MOLECULAR THERAPY

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

Zinc-finger Nucleases as a Novel Therapeutic Strategy for Targeting Hepatitis B Virus DNAs

(2010) Thomas J Cradick et al.   MOLECULAR THERAPY

Targeted chromosomal deletions in human cells using zinc finger nucleases

(2009) H. J. Lee et al.   GENOME RESEARCH