Computational design of a specific heavy chain/κ light chain interface for expressing fully IgG bispecific antibodies

The making of bispecific antibodies

(2017) Ulrich Brinkmann et al.   mAbs

Enhanced tumor-targeting selectivity by modulating bispecific antibody binding affinity and format valence

(2017) Yariv Mazor et al.   Scientific Reports

Bispecific antibodies and CARs: generalized immunotherapeutics harnessing T cell redirection

(2016) Eugene A Zhukovsky et al.   CURRENT OPINION IN IMMUNOLOGY

Comparing domain interactions within antibody Fabs with kappa and lambda light chains

(2016) Raheleh Toughiri et al.   mAbs

Despite slow progress, bispecifics generate buzz

(2016) Cormac Sheridan   NATURE BIOTECHNOLOGY

Computationally Designed Bispecific Antibodies using Negative State Repertoires

(2016) Andrew Leaver-Fay et al.   STRUCTURE

Immunoglobulin Fc Heterodimer Platform Technology: From Design to Applications in Therapeutic Antibodies and Proteins

(2016) Ji-Hee Ha et al.   Frontiers in Immunology

A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: Process development and product quality assessment

(2015) Yashas Rajendra et al.   BIOTECHNOLOGY AND BIOENGINEERING

Transcriptional and post-transcriptional targeting for enhanced transient gene expression in CHO cells

(2015) Yashas Rajendra et al.   BIOTECHNOLOGY LETTERS

Enhanced plasmid DNA utilization in transiently transfected CHO-DG44 cells in the presence of polar solvents

(2015) Yashas Rajendra et al.   BIOTECHNOLOGY PROGRESS

Unexpected hepatotoxicity in a phase I study of TAS266, a novel tetravalent agonistic Nanobody® targeting the DR5 receptor

(2015) Kyriakos P. Papadopoulos et al.   CANCER CHEMOTHERAPY AND PHARMACOLOGY

Bispecific antibodies

(2015) Roland E. Kontermann et al.   DRUG DISCOVERY TODAY

A Novel Antibody Engineering Strategy for Making Monovalent Bispecific Heterodimeric IgG Antibodies by Electrostatic Steering Mechanism

(2015) Zhi Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Combined Covalent-Electrostatic Model of Hydrogen Bonding Improves Structure Prediction with Rosetta

(2015) Matthew J. O’Meara et al.   Journal of Chemical Theory and Computation

Fab-based bispecific antibody formats with robust biophysical properties and biological activity

(2015) Xiufeng Wu et al.   mAbs

Improving target cell specificity using a novel monovalent bispecific IgG design

(2015) Yariv Mazor et al.   mAbs

Alternative molecular formats and therapeutic applications for bispecific antibodies

(2015) Christoph Spiess et al.   MOLECULAR IMMUNOLOGY

Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG

(2015) Nicolas Fischer et al.   Nature Communications

Antiparallel Conformation of Knob and Hole Aglycosylated Half-Antibody Homodimers Is Mediated by a CH2–CH3 Hydrophobic Interaction

(2014) J.Michael Elliott et al.   JOURNAL OF MOLECULAR BIOLOGY

Generation of bispecific IgG antibodies by structure-based design of an orthogonal Fab interface

(2014) Steven M Lewis et al.   NATURE BIOTECHNOLOGY

Autoantibodies to Variable Heavy (VH) Chain Ig Sequences in Humans Impact the Safety and Clinical Pharmacology of a VH Domain Antibody Antagonist of TNF-α Receptor 1

(2013) M. C. Holland et al.   JOURNAL OF CLINICAL IMMUNOLOGY

Improving biophysical properties of a bispecific antibody scaffold to aid developability

(2013) Thomas Spreter Von Kreudenstein et al.   mAbs

Bispecific antibodies with natural architecture produced by co-culture of bacteria expressing two distinct half-antibodies

(2013) Christoph Spiess et al.   NATURE BIOTECHNOLOGY

Efficient generation of stable bispecific IgG1 by controlled Fab-arm exchange

(2013) A. F. Labrijn et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generating Bispecific Human IgG1 and IgG2 Antibodies from Any Antibody Pair

(2012) Pavel Strop et al.   JOURNAL OF MOLECULAR BIOLOGY

Progress in overcoming the chain association issue in bispecific heterodimeric IgG antibodies

(2012) Christian Klein et al.   mAbs

Targeting T Cells with Bispecific Antibodies for Cancer Therapy

(2011) Lawrence G. Lum et al.   BIODRUGS

Anchored Design of Protein-Protein Interfaces

(2011) Steven M. Lewis et al.   PLoS One

A Generic Program for Multistate Protein Design

(2011) Andrew Leaver-Fay et al.   PLoS One

Immunoglobulin domain crossover as a generic approach for the production of bispecific IgG antibodies

(2011) W. Schaefer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enhancing Antibody Fc Heterodimer Formation through Electrostatic Steering Effects

(2010) Kannan Gunasekaran et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Alternate States of Proteins Revealed by Detailed Energy Landscape Mapping

(2010) Michael D. Tyka et al.   JOURNAL OF MOLECULAR BIOLOGY

Anti-tumor activity of stability-engineered IgG-like bispecific antibodies targeting TRAIL-R2 and LTβR

(2010) Jennifer S. Michaelson et al.   mAbs

Bispecific antibodies for cancer therapy

(2010) Patrick Chames et al.   mAbs

Role of conformational sampling in computing mutation-induced changes in protein structure and stability

(2010) Elizabeth H. Kellogg et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Characterization of Inhibitory Anti-insulin-like Growth Factor Receptor Antibodies with Different Epitope Specificity and Ligand-blocking Properties

(2009) Adam Doern et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

An Unfolded CH1 Domain Controls the Assembly and Secretion of IgG Antibodies

(2009) Matthias J. Feige et al.   MOLECULAR CELL

Therapeutic IgG4 antibodies engage in Fab-arm exchange with endogenous human IgG4 in vivo

(2009) Aran F Labrijn et al.   NATURE BIOTECHNOLOGY

Structural understanding of stabilization patterns in engineered bispecific Ig-like antibody molecules

(2009) Jacob L. Jordan et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Matuzumab Binding to EGFR Prevents the Conformational Rearrangement Required for Dimerization

(2008) Judith Schmiedel et al.   CANCER CELL

MetMAb, the One-Armed 5D5 Anti-c-Met Antibody, Inhibits Orthotopic Pancreatic Tumor Growth and Improves Survival

(2008) H. Jin et al.   CANCER RESEARCH