Transcriptional and post-transcriptional targeting for enhanced transient gene expression in CHO cells

A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: Process development and product quality assessment

(2015) Yashas Rajendra et al.   BIOTECHNOLOGY AND BIOENGINEERING

Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells

(2015) Yashas Rajendra et al.   BIOTECHNOLOGY PROGRESS

The endoplasmic reticulum and unfolded protein response in the control of mammalian recombinant protein production

(2014) Hirra Hussain et al.   BIOTECHNOLOGY LETTERS

High-throughput mAb expression and purification platform based on transient CHO

(2014) Gavin C. Barnard et al.   BIOTECHNOLOGY PROGRESS

Use of an anti-apoptotic CHO cell line for transient gene expression

(2013) Nichole F. Macaraeg et al.   BIOTECHNOLOGY PROGRESS

A CHO cell line engineered to express XBP1 and ERO1-Lα has increased levels of transient protein expression

(2013) Katharine Cain et al.   BIOTECHNOLOGY PROGRESS

A high-yielding CHO transient system: Coexpression of genes encoding EBNA-1 and GS enhances transient protein expression

(2013) Olalekan Daramola et al.   BIOTECHNOLOGY PROGRESS

Improved antibody production in Chinese hamster ovary cells by ATF4 overexpression

(2013) Ahmad M. Haredy et al.   CYTOTECHNOLOGY

Overexpression of CHOP alone and in combination with chaperones is effective in improving antibody production in mammalian cells

(2012) Daisuke Nishimiya et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells

(2012) Yashas Rajendra et al.   BIOTECHNOLOGY AND BIOENGINEERING

Maximizing productivity of CHO cell-based fed-batch culture using chemically defined media conditions and typical manufacturing equipment

(2010) Yao-Ming Huang et al.   BIOTECHNOLOGY PROGRESS

Analyzing real-time PCR data by the comparative CT method

(2008) Thomas D Schmittgen et al.   Nature Protocols

Improved production of recombinant human antithrombin III in Chinese hamster ovary cells by ATF4 overexpression

(2007) Tomoshi Ohya et al.   BIOTECHNOLOGY AND BIOENGINEERING