Detailed comparison of retroviral vectors and promoter configurations for stable and high transgene expression in human induced pluripotent stem cells

Alpharetroviral self-inactivating vectors produced by a superinfection-resistant stable packaging cell line allow genetic modification of primary human T lymphocytes

(2016) Verena Labenski et al.   BIOMATERIALS

Gene Insertion Into Genomic Safe Harbors for Human Gene Therapy

(2016) Eirini P Papapetrou et al.   MOLECULAR THERAPY

Efficient generation of gene-modified human natural killer cells via alpharetroviral vectors

(2015) Julia D. Suerth et al.   JOURNAL OF MOLECULAR MEDICINE-JMM

Gene therapy returns to centre stage

(2015) Luigi Naldini   NATURE

A minimal ubiquitous chromatin opening element (UCOE) effectively prevents silencing of juxtaposed heterologous promoters by epigenetic remodeling in multipotent and pluripotent stem cells

(2015) Uta Müller-Kuller et al.   NUCLEIC ACIDS RESEARCH

Efficient Recombinase-Mediated Cassette Exchange in hPSCs to Study the Hepatocyte Lineage Reveals AAVS1 Locus-Mediated Transgene Inhibition

(2015) Laura Ordovás et al.   Stem Cell Reports

Mystery solved: VSV-G-LVs do not allow efficient gene transfer into unstimulated T cells, B cells, and HSCs because they lack the LDL receptor

(2014) F. Amirache et al.   BLOOD

Improved retroviral episome transfer of transcription factors enables sustained cell fate modification

(2014) J W Schott et al.   GENE THERAPY

Modified Lentiviral LTRs Allow Flp Recombinase–mediated Cassette Exchange and In Vivo Tracing of “Factor-free” Induced Pluripotent Stem Cells

(2014) Johannes Kuehle et al.   MOLECULAR THERAPY

A Modified γ-Retrovirus Vector for X-Linked Severe Combined Immunodeficiency

(2014) Salima Hacein-Bey-Abina et al.   NEW ENGLAND JOURNAL OF MEDICINE

Reprogrammed Cells for Disease Modeling and Regenerative Medicine

(2013) Anne B.C. Cherry et al.   Annual Review of Medicine

Functionality of Endothelial Cells and Pericytes From Human Pluripotent Stem Cells Demonstrated in Cultured Vascular Plexus and Zebrafish Xenografts

(2013) Valeria V. Orlova et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Promoter and lineage independent anti-silencing activity of the A2 ubiquitous chromatin opening element for optimized human pluripotent stem cell-based gene therapy

(2013) Mania Ackermann et al.   BIOMATERIALS

Genetic correction of HAX1 in induced pluripotent stem cells from a patient with severe congenital neutropenia improves defective granulopoiesis

(2013) T. Morishima et al.   HAEMATOLOGICA

Optimizing Retroviral Gene Expression for Effective Therapies

(2013) Michael N. Antoniou et al.   HUMAN GENE THERAPY

Kinetics and Epigenetics of Retroviral Silencing in Mouse Embryonic Stem Cells Defined by Deletion of the D4Z4 Element

(2013) Sylvie Rival-Gervier et al.   MOLECULAR THERAPY

LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis virus

(2013) D. Finkelshtein et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lentiviral Hematopoietic Stem Cell Gene Therapy in Patients with Wiskott-Aldrich Syndrome

(2013) A. Aiuti et al.   SCIENCE

A ubiquitous chromatin opening element prevents transgene silencing in pluripotent stem cells and their differentiated progeny

(2013) Nils Pfaff et al.   STEM CELLS

Proviral Silencing in Embryonic Cells Is Regulated by Yin Yang 1

(2013) Sharon Schlesinger et al.   Cell Reports

Extensive Methylation of Promoter Sequences Silences Lentiviral Transgene Expression During Stem Cell Differentiation In Vivo

(2012) Friederike Herbst et al.   MOLECULAR THERAPY

Alpharetroviral Self-inactivating Vectors: Long-term Transgene Expression in Murine Hematopoietic Cells and Low Genotoxicity

(2012) Julia D Suerth et al.   MOLECULAR THERAPY

Alpharetroviral Vector-mediated Gene Therapy for X-CGD: Functional Correction and Lack of Aberrant Splicing

(2012) Kerstin B. Kaufmann et al.   MOLECULAR THERAPY

The Impact of cHS4 Insulators on DNA Transposon Vector Mobilization and Silencing in Retinal Pigment Epithelium Cells

(2012) Nynne Sharma et al.   PLoS One

The Use of Chromatin Insulators to Improve the Expression and Safety of Integrating Gene Transfer Vectors

(2011) David W. Emery   HUMAN GENE THERAPY

Lentiviral Vector Design and Imaging Approaches to Visualize the Early Stages of Cellular Reprogramming

(2011) Eva Warlich et al.   MOLECULAR THERAPY

Generation and genetic modification of induced pluripotent stem cells

(2010) Axel Schambach et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Self-Inactivating Alpharetroviral Vectors with a Split-Packaging Design

(2010) J. D. Suerth et al.   JOURNAL OF VIROLOGY

A Ubiquitous Chromatin Opening Element (UCOE) Confers Resistance to DNA Methylation–mediated Silencing of Lentiviral Vectors

(2010) Fang Zhang et al.   MOLECULAR THERAPY

Genomic safe harbors permit high β-globin transgene expression in thalassemia induced pluripotent stem cells

(2010) Eirini P Papapetrou et al.   NATURE BIOTECHNOLOGY

Quantitative Comparison of Constitutive Promoters in Human ES cells

(2010) Karin Norrman et al.   PLoS One

Protein transduction from retroviral Gag precursors

(2010) Christine Voelkel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mechanism of Reduction in Titers From Lentivirus Vectors Carrying Large Inserts in the 3′LTR

(2009) Fabrizia Urbinati et al.   MOLECULAR THERAPY

Embryonic stem cells use ZFP809 to silence retroviral DNAs

(2009) Daniel Wolf et al.   NATURE

Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells

(2009) Ángel Raya et al.   NATURE

Instability of Retroviral DNA Methylation in Embryonic Stem Cells

(2008) Shigeru Minoguchi et al.   STEM CELLS