A minimal ubiquitous chromatin opening element (UCOE) effectively prevents silencing of juxtaposed heterologous promoters by epigenetic remodeling in multipotent and pluripotent stem cells

Uncovering and Dissecting the Genotoxicity of Self-inactivating Lentiviral Vectors In Vivo

(2014) Daniela Cesana et al.   MOLECULAR THERAPY

CTCF: an architectural protein bridging genome topology and function

(2014) Chin-Tong Ong et al.   NATURE REVIEWS GENETICS

PADI4 acts as a coactivator of Tal1 by counteracting repressive histone arginine methylation

(2014) Stephan Kolodziej et al.   Nature Communications

Promoter and lineage independent anti-silencing activity of the A2 ubiquitous chromatin opening element for optimized human pluripotent stem cell-based gene therapy

(2013) Mania Ackermann et al.   BIOMATERIALS

Optimizing Retroviral Gene Expression for Effective Therapies

(2013) Michael N. Antoniou et al.   HUMAN GENE THERAPY

Biosafety Features of Lentiviral Vectors

(2013) Axel Schambach et al.   HUMAN GENE THERAPY

A ubiquitous chromatin opening element prevents transgene silencing in pluripotent stem cells and their differentiated progeny

(2013) Nils Pfaff et al.   STEM CELLS

Insulators Target Active Genes to Transcription Factories and Polycomb-Repressed Genes to Polycomb Bodies

(2013) Hua-Bing Li et al.   PLoS Genetics

The chromatin backdrop of DNA replication: Lessons from genetics and genome-scale analyses

(2012) Amy L. Conner et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

Whole transcriptome characterization of aberrant splicing events induced by lentiviral vector integrations

(2012) Daniela Cesana et al.   JOURNAL OF CLINICAL INVESTIGATION

Safer, Silencing-Resistant Lentiviral Vectors: Optimization of the Ubiquitous Chromatin-Opening Element through Elimination of Aberrant Splicing

(2012) S. Knight et al.   JOURNAL OF VIROLOGY

Extensive Methylation of Promoter Sequences Silences Lentiviral Transgene Expression During Stem Cell Differentiation In Vivo

(2012) Friederike Herbst et al.   MOLECULAR THERAPY

Foamy Virus Vector-mediated Gene Correction of a Mouse Model of Wiskott–Aldrich Syndrome

(2012) Toru Uchiyama et al.   MOLECULAR THERAPY

MicroRNA-150-regulated vectors allow lymphocyte-sparing transgene expression in hematopoietic gene therapy

(2011) N Lachmann et al.   GENE THERAPY

Physiological regulation of transgene expression by a lentiviral vector containing the A2UCOE linked to a myeloid promoter

(2011) C Brendel et al.   GENE THERAPY

The Use of Chromatin Insulators to Improve the Expression and Safety of Integrating Gene Transfer Vectors

(2011) David W. Emery   HUMAN GENE THERAPY

Daedalus: a robust, turnkey platform for rapid production of decigram quantities of active recombinant proteins in human cell lines using novel lentiviral vectors

(2011) Ashok D. Bandaranayake et al.   NUCLEIC ACIDS RESEARCH

Efficient Hematopoietic Redifferentiation of Induced Pluripotent Stem Cells Derived from Primitive Murine Bone Marrow Cells

(2011) Nils Pfaff et al.   STEM CELLS AND DEVELOPMENT

A Ubiquitous Chromatin Opening Element (UCOE) Confers Resistance to DNA Methylation–mediated Silencing of Lentiviral Vectors

(2010) Fang Zhang et al.   MOLECULAR THERAPY

CpG islands influence chromatin structure via the CpG-binding protein Cfp1

(2010) John P. Thomson et al.   NATURE

Transfusion independence and HMGA2 activation after gene therapy of human β-thalassaemia

(2010) Marina Cavazzana-Calvo et al.   NATURE

CpG methylation of half-CRE sequences creates C/EBP  binding sites that activate some tissue-specific genes

(2010) V. Rishi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Incorporating double copies of a chromatin insulator into lentiviral vectors results in less viral integrants

(2009) Troels T Nielsen et al.   BMC BIOTECHNOLOGY

Simplified lentivirus vector production in protein-free media using polyethylenimine-mediated transfection

(2009) Hitoshi Kuroda et al.   JOURNAL OF VIROLOGICAL METHODS

Mechanism of Reduction in Titers From Lentivirus Vectors Carrying Large Inserts in the 3′LTR

(2009) Fabrizia Urbinati et al.   MOLECULAR THERAPY

Desmin-regulated Lentiviral Vectors for Skeletal Muscle Gene Transfer

(2009) Gillian E Talbot et al.   MOLECULAR THERAPY

Retroviral vector silencing during iPS cell induction: An epigenetic beacon that signals distinct pluripotent states

(2008) Akitsu Hotta et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Instability of Retroviral DNA Methylation in Embryonic Stem Cells

(2008) Shigeru Minoguchi et al.   STEM CELLS