The Use of Chromatin Insulators to Improve the Expression and Safety of Integrating Gene Transfer Vectors

A self-inactivating lentiviral vector for SCID-X1 gene therapy that does not activate LMO2 expression in human T cells

(2010) S. Zhou et al.   BLOOD

A functional screen for regulatory elements that improve retroviral vector gene expression

(2010) Amy C. Groth et al.   BLOOD CELLS MOLECULES AND DISEASES

Poly(ADP-ribose) Polymerase-1 (PARP-1) Contributes to the Barrier Function of a Vertebrate Chromatin Insulator

(2010) Mari Aker et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Large double copy vectors are functional but show a size-dependent decline in transduction efficiency

(2010) Tomas J. Bos et al.   JOURNAL OF BIOTECHNOLOGY

Mutation of a barrier insulator in the human ankyrin-1 gene is associated with hereditary spherocytosis

(2010) Patrick G. Gallagher et al.   JOURNAL OF CLINICAL INVESTIGATION

Transfusion independence and HMGA2 activation after gene therapy of human β-thalassaemia

(2010) Marina Cavazzana-Calvo et al.   NATURE

Insulators and promoters: closer than we think

(2010) Jesse R. Raab et al.   NATURE REVIEWS GENETICS

Cell type specificity of chromatin organization mediated by CTCF and cohesin

(2010) Chunhui Hou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

VEZF1 Elements Mediate Protection from DNA Methylation

(2010) Jacqueline Dickson et al.   PLoS Genetics

CTCF: Master Weaver of the Genome

(2009) Jennifer E. Phillips et al.   CELL

The genotoxic potential of retroviral vectors is strongly modulated by vector design and integration site selection in a mouse model of HSC gene therapy

(2009) Eugenio Montini et al.   JOURNAL OF CLINICAL INVESTIGATION

Mutational Analysis of the Poly(ADP-Ribosyl)ation Sites of the Transcription Factor CTCF Provides an Insight into the Mechanism of Its Regulation by Poly(ADP-Ribosyl)ation

(2009) D. Farrar et al.   MOLECULAR AND CELLULAR BIOLOGY

Optimized Lentiviral Vector Design Improves Titer and Transgene Expression of Vectors Containing the Chicken β-Globin Locus HS4 Insulator Element

(2009) Hideki Hanawa et al.   MOLECULAR THERAPY

Genotoxic Potential of Lineage-specific Lentivirus Vectors Carrying the β-Globin Locus Control Region

(2009) Paritha I Arumugam et al.   MOLECULAR THERAPY

The Sea Urchin sns5 Insulator Protects Retroviral Vectors From Chromosomal Position Effects by Maintaining Active Chromatin Structure

(2009) Danilo D'Apolito et al.   MOLECULAR THERAPY

Mechanism of Reduction in Titers From Lentivirus Vectors Carrying Large Inserts in the 3′LTR

(2009) Fabrizia Urbinati et al.   MOLECULAR THERAPY

Genomic and Functional Assays Demonstrate Reduced Gammaretroviral Vector Genotoxicity Associated With Use of the cHS4 Chromatin Insulator

(2009) Chang Long Li et al.   MOLECULAR THERAPY

H3.3/H2A.Z double variant–containing nucleosomes mark 'nucleosome-free regions' of active promoters and other regulatory regions

(2009) Chunyuan Jin et al.   NATURE GENETICS

Gene Specificity of Suppression of Transgene-Mediated Insertional Transcriptional Activation by the Chicken HS4 Insulator

(2009) Romain Desprat et al.   PLoS One

The 3′ Region of the Chicken Hypersensitive Site-4 Insulator Has Properties Similar to Its Core and Is Required for Full Insulator Activity

(2009) Paritha I. Arumugam et al.   PLoS One

Transcriptional control by PARP-1: chromatin modulation, enhancer-binding, coregulation, and insulation

(2008) W Lee Kraus   CURRENT OPINION IN CELL BIOLOGY

Physiological Promoters Reduce the Genotoxic Risk of Integrating Gene Vectors

(2008) Daniela Zychlinski et al.   MOLECULAR THERAPY

Globin Lentiviral Vector Insertions Can Perturb the Expression of Endogenous Genes in β-thalassemic Hematopoietic Cells

(2008) Phillip W Hargrove et al.   MOLECULAR THERAPY

Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions

(2008) Lars Guelen et al.   NATURE

CTCF physically links cohesin to chromatin

(2008) E. D. Rubio et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Combinatorial Incorporation of Enhancer-Blocking Components of the Chicken β-Globin 5′HS4 and Human T-Cell Receptor α/δ BEAD-1 Insulators in Self-Inactivating Retroviral Vectors Reduces Their Genotoxic Potential

(2008) Ali Ramezani et al.   STEM CELLS

Transcriptional Activities of the Sleeping Beauty Transposon and Shielding Its Genetic Cargo With Insulators

(2007) Oliver Walisko et al.   MOLECULAR THERAPY