Engineering yeast for producing human glycoproteins: where are we now?

In vitro enzymatic treatment to remove O-linked mannose from intact glycoproteins

(2014) Sujatha Gomathinayagam et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Genentech's glyco-engineered antibody to succeed Rituxan

(2014) Mark Ratner   NATURE BIOTECHNOLOGY

Glycoengineering of yeasts from the perspective of glycosylation efficiency

(2014) Mari A. Piirainen et al.   New Biotechnology

Sweet and Sour: The Impact of Differential Glycosylation in Cancer Cells Undergoing Epithelial–Mesenchymal Transition

(2014) Leonardo Freire-de-Lima   Frontiers in Oncology

Production of sialylated O-linked glycans in Pichia pastoris

(2013) S. R. Hamilton et al.   GLYCOBIOLOGY

Characterization of the Pichia pastoris Protein-O-mannosyltransferase Gene Family

(2013) Juergen H. Nett et al.   PLoS One

PMT1 gene plays a major role in O-mannosylation of insulin precursor in Pichia pastoris

(2013) Nagaraj Govindappa et al.   PROTEIN EXPRESSION AND PURIFICATION

Production of biopharmaceutical proteins by yeast

(2013) Jens Nielsen   Bioengineered

A Combined System for Engineering Glycosylation Efficiency and Glycan Structure in Saccharomyces cerevisiae

(2012) Farnoush Parsaie Nasab et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Improvement of N-glycan site occupancy of therapeutic glycoproteins produced in Pichia pastoris

(2012) Byung-Kwon Choi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

High abundance of Serine/Threonine-rich regions predicted to be hyper-O-glycosylated in the secretory proteins coded by eight fungal genomes

(2012) Mario González et al.   BMC MICROBIOLOGY

MUC1 immunotherapy is here to stay

(2012) Takashi Kimura et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Identification of Novel α1,3-Galactosyltransferase and Elimination of α-Galactose-containing Glycans by Disruption of Multiple α-Galactosyltransferase Genes inSchizosaccharomyces pombe

(2012) Takao Ohashi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Marketing approval of mogamulizumab

(2012) Alain Beck et al.   mAbs

Engineering the yeast Yarrowia lipolytica for the production of therapeutic proteins homogeneously glycosylated with Man8GlcNAc2 and Man5GlcNAc2

(2012) Karen De Pourcq et al.   Microbial Cell Factories

A bacterial glycosidase enables mannose-6-phosphate modification and improved cellular uptake of yeast-produced recombinant human lysosomal enzymes

(2012) Petra Tiels et al.   NATURE BIOTECHNOLOGY

Vertebrate protein glycosylation: diversity, synthesis and function

(2012) Kelley W. Moremen et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

The Impact of Glycosylation on the Pharmacokinetics of a TNFR2:Fc Fusion Protein Expressed in Glycoengineered Pichia Pastoris

(2012) Liming Liu et al.   PHARMACEUTICAL RESEARCH

Engineering Yarrowia lipolytica to Produce Glycoproteins Homogeneously Modified with the Universal Man3GlcNAc2 N-Glycan Core

(2012) Karen De Pourcq et al.   PLoS One

Expression of glycoproteins bearing complex human-like glycans with galactose terminal in Hansenula polymorpha

(2012) Hui Wang et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Elimination of  -mannose glycan structures in Pichia pastoris

(2011) D. Hopkins et al.   GLYCOBIOLOGY

Optimization of erythropoietin production with controlled glycosylation-PEGylated erythropoietin produced in glycoengineered Pichia pastoris

(2011) Juergen H. Nett et al.   JOURNAL OF BIOTECHNOLOGY

Biochemical and biophysical characterization of humanized IgG1 produced inPichia pastoris

(2011) Sha Ha et al.   mAbs

Glycoengineered Pichia produced anti-HER2 is comparable to trastuzumab in preclinical study

(2011) Ningyan Zhang et al.   mAbs

Functional and genomic analyses of blocked protein O-mannosylation in baker's yeast

(2011) Javier Arroyo et al.   MOLECULAR MICROBIOLOGY

Essential Role of YlMPO1, a NovelYarrowia lipolyticaHomologue ofSaccharomyces cerevisiae MNN4, in Mannosylphosphorylation of N- and O-Linked Glycans

(2010) Jeong-Nam Park et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Recombinant Glucocerebrosidase (Imiglucerase) as a Therapy for Gaucher Disease

(2010) Gregory M. Pastores   BIODRUGS

Glycosylation of Therapeutic Proteins

(2010) Ricardo J. Solá et al.   BIODRUGS

Identification and functional characterization of the HpALG11 and the HpRFT1 genes involved in N-linked glycosylation in the methylotrophic yeast Hansenula polymorpha

(2010) Haolei Song et al.   GLYCOBIOLOGY

Biopharmaceutical benchmarks 2010

(2010) Gary Walsh   NATURE BIOTECHNOLOGY

N- and O-linked oligosaccharides completely lack galactose residues in the gms1och1 mutant of Schizosaccharomyces pombe

(2009) Takao Ohashi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Pharmacological significance of glycosylation in therapeutic proteins

(2009) Huijuan Li et al.   CURRENT OPINION IN BIOTECHNOLOGY

Development of valuable yeast strains using a novel mutagenesis technique for the effective production of therapeutic glycoproteins

(2009) H. Abe et al.   GLYCOBIOLOGY

Production of human  -hexosaminidase A with highly phosphorylated N-glycans by the overexpression of the Ogataea minuta MNN4 gene

(2009) H. Akeboshi et al.   GLYCOBIOLOGY

Protein O-mannosylation: Conserved from bacteria to humans

(2009) M. Lommel et al.   GLYCOBIOLOGY

Carbohydrate analysis throughout the development of a protein therapeutic

(2009) Elizabeth Higgins   GLYCOCONJUGATE JOURNAL

Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology

(2009) Pieter P Jacobs et al.   Nature Protocols

Glycans on influenza hemagglutinin affect receptor binding and immune response

(2009) C.-C. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Glycoengineering of the methylotrophic yeastHansenula polymorpha for the production of glycoproteins with trimannosyl coreN-glycan by blocking core oligosaccharide assembly

(2008) Doo-Byoung Oh et al.   Biotechnology Journal

Engineering of a mammalian O-glycosylation pathway in the yeast Saccharomyces cerevisiae: production of O-fucosylated epidermal growth factor domains

(2008) Y. Chigira et al.   GLYCOBIOLOGY

Engineering of mucin-type human glycoproteins in yeast cells

(2008) K. Amano et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA