Article
Horticulture
Jung Su Jo, Shiva Ram Bhandari, Gwan Ho Kang, Yu Kyeong Shin, Jun Gu Lee
Summary: This study investigated the GSL profile, hydrolysate and nitrile contents, and nitrile formation rate of three commercial cultivars and eleven inbred broccoli lines. The results showed that glucobrassicin and glucoraphanin were the most dominant GSLs in all lines. Based on GSL content, five lines were selected as potential highly functional broccoli lines. Three lines exhibited relatively higher total hydrolysates. Three inbred lines with low nitrile formation rate and high isothiocyanate formation were selected as functional broccoli breeding lines. Therefore, hydrolysate and GSL content are deemed more reliable for selecting and developing highly functional varieties.
SCIENTIA HORTICULTURAE
(2022)
Article
Biochemistry & Molecular Biology
Junwei Wang, Shuxiang Mao, Mantian Liang, Wenxia Zhang, Fangzhen Chen, Ke Huang, Qiuyun Wu
Summary: This study found that exogenous methyl jasmonate (MeJA) treatment can promote glucosinolate (GSL) biosynthesis and sulforaphane accumulation in broccoli, with a more pronounced effect on florets. Additionally, MeJA treatment increases flavonoid content and antioxidant activity in broccoli florets.
Article
Agriculture, Multidisciplinary
Cui Li, Shuhui Song, Yanan He, Siyu Han, Haijie Liu
Summary: A new type of electrolyzed water, CaCl2-HCl electrolyzed water, was found to enhance glucosinolate metabolism in broccoli sprouts by activating calcium signaling pathway. This research provides a theoretical basis for cultivating high-quality broccoli sprouts.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2023)
Article
Nutrition & Dietetics
John A. Bouranis, Laura M. Beaver, Jaewoo Choi, Carmen P. Wong, Duo Jiang, Thomas J. Sharpton, Jan F. Stevens, Emily Ho
Summary: Research indicates that the production of isothiocyanates may be influenced by the composition of the gut microbiome, potentially impacting the efficacy of interventions with cruciferous vegetables. In humans, there is significant inter-individual variability in SFN-NIT excretion following consumption of broccoli sprouts.
Article
Food Science & Technology
Christopher Chen, Ryun Hee Kim, Keum Taek Hwang, Jaecheol Kim
Summary: This study aims to measure the chemical compounds and bioactivities of radish sprout extracts exposed to red and blue LED during cultivation. The results showed that red LED exposure increased the total phenolic content, total flavonoid content, and glucoraphasatin content in the extracts. Red LED also enhanced the antioxidant capacities of the sprouts. On the other hand, blue LED exposure effectively promoted the synthesis of substances with in vitro tyrosinase inhibitory effects in the sprouts.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Henryk Debski, Wieslaw Wiczkowski, Joanna Szablinska-Piernik, Marcin Horbowicz
Summary: The effects of elicitors on broccoli and radish sprouts were evaluated in this study. It was found that radish sprouts have higher antioxidant activity compared to broccoli sprouts, likely due to their higher total phenolic acids content. The applied elicitors increased the total phenolic acids and antioxidant activity in both radish and broccoli sprouts, while decreasing the total flavonoids in broccoli sprouts.
Article
Chemistry, Applied
Lars Andernach, Katja Witzel, Franziska S. Hanschen
Summary: Glucosinolates are precursors of isothiocyanates (ITCs). Amines were found to be enzymatic hydrolysis products of glucosinolates in Brassica vegetables. An endogenous ITC hydrolase (ITCase) in the plants is responsible for the conversion of ITCs to amines. The presence of amines from glucosinolates in the human diet was confirmed.
Article
Nutrition & Dietetics
Joseph Yusin, Vivian Wang, Susanne M. Henning, Jieping Yang, Chi-Hong Tseng, Gail Thames, Irina Arnold, David Heber, Ru-Po Lee, Laura Sanavio, Yajing Pan, Tianyu Qin, Zhaoping Li
Summary: Combining nasal corticosteroid with broccoli sprout extract led to the most significant improvement in peak nasal inspiratory flow and total nasal symptoms scores in patients diagnosed with grass-induced allergic rhinitis.
Article
Chemistry, Multidisciplinary
Yongqi Yin, Yin Liu, Chao Cheng, Zhengfei Yang, Zhenlan Luo, Weiming Fang
Summary: Exogenous melatonin enhances the tolerance of broccoli sprouts to ZnSO4 stress, promotes glucoraphanin biosynthesis, and increases the content of isothiocyanates. The molecular mechanism underlying the role of melatonin in isothiocyanate metabolism under ZnSO4 stress was investigated using physio-biochemical methods, quantification of gene expression levels, and iTRAQ labelling technique.
Article
Agronomy
Betiana S. Trod, Melina S. Buttarelli, Maria M. Stoffel, Gabriel Ceccoli, Laura Olivella, Pamela B. Barengo, Merce Llugany, Maria G. Guevara, Fernando F. Munoz, Lucas D. Daurelio
Summary: Broccoli heads are valuable vegetable crops due to their high nutritional content, but they quickly decompose after harvest. Previous research has shown that foliar application of sodium selenate and selenite can delay this process. This study analyzed the effect of these preharvest treatments on the postharvest shelf-life of broccoli and found that selenium treatments not only delayed yellowing but also maintained the firmness, diameter, and nutritional composition of the heads.
Article
Chemistry, Applied
Junwei Wang, Shuxiang Mao, Qi Wu, Yiming Yuan, Mantian Liang, Shengze Wang, Ke Huang, Qiuyun Wu
Summary: Red light promotes GSL biosynthesis and sulforaphane accumulation, while blue light inhibits them. LED light sources regulate the biosynthesis of GSLs and sulforaphane in broccoli through the modulation of gene expression and metabolic pathways.
Article
Chemistry, Applied
Yongbo Duan, Franklin Eduardo Melo Santiago, Andre Rodrigues dos Reis, Marislaine A. de Figueiredo, Suping Zhou, Theodore W. Thannhauser, Li Li
Summary: Flavonols, including kaempferol and quercetin, showed significant variation in broccoli accessions, with higher accumulation of kaempferol in leaves and quercetin in florets. Total flavonoid content also varied greatly among different accessions. Principle component analysis indicated a correlation between flavonols, biosynthetic pathway genes, and antioxidant capacity indicators in broccoli.
Article
Chemistry, Applied
Yao Zhang, Nyasha Makaza, Chunmin Jiang, Yaqing Wu, Sabir Z. Nishanbaev, Ligen Zou, Juan Sun, Xinjie Song, Yuanfeng Wu
Summary: Brassica vegetables, especially broccoli, have health benefits due to their anticancer activity, which is attributed to isothiocyanate (ITC) compounds. This study found that the cooking time and addition of exogenous myrosinase (MYR) can affect the yield of ITCs. Raw broccoli had a high amount of ITCs, but microwaving for 1 minute decreased it by almost 40%. Adding ground moringa seeds to cooked broccoli caused a notable increase of 38% in ITC content.
Article
Chemistry, Applied
Yu-Hsiang Wu, Yan-Han Lin, Chung-Yi Wang
Summary: The effects of high-pressure processing (HPP) on kale leaves were studied, including myrosinase activity, glucosinolate (GLS) content, isothiocyanate (ITC) conversion rate, color, and bacterial count. The sample processed at 600 MPa showed the highest myrosinase activity and ITC conversion rate, but the GLS content was significantly lower. HPP induced crushing damage to the leaves, promoting the conversion process, and caused minimal color change. HPP achieved the same level of pasteurization as thermal treatment in terms of bacterial count.
Article
Agriculture, Multidisciplinary
Yu Tie, Wenyou Zhu, Chao Zhang, Liguo Yin, Yalin Zhang, Linpei Liu, Huawei Yuan
Summary: In this study, a bacterial strain identified as Leclercia adecarboxylata was isolated from the rhizosphere soil of rape seedlings, which harbored two myrosinase genes and an ITC hydrolase gene. It was demonstrated that these myrosinases play a crucial role in the utilization of GSLs, and the metallo-beta-lactamase 3836 may be involved in the bacterium's tolerance mechanism to allyl-ITC.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Applied
Pei Wang, Cuidan Hou, Xiaohui Zhao, Mengqi Tian, Zhenxin Gu, Runqiang Yang
FOOD HYDROCOLLOIDS
(2019)
Article
Agriculture, Multidisciplinary
Runqiang Yang, Qianru Hui, Xiaoyun Feng, Li Feng, Zhenxin Gu, Pei Wang
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2020)
Article
Chemistry, Applied
Ting Zhou, Pei Wang, Zhenxin Gu, Meng Ma, Runqiang Yang
Article
Agriculture, Multidisciplinary
Pei Wang, Xiaohui Zhao, Runqiang Yang, Yulin Zhou, Qin Zhou, Zhenxin Gu, Dong Jiang
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Mian Wang, Yahui Zhu, Pei Wang, Zhenxin Gu, Runqiang Yang
Summary: It has been found that low concentration of NaCl not only promotes growth of barley seedlings, but also stimulates endogenous GABA metabolism to affect key enzyme activities and mRNA levels for phenolics synthesis.
Article
Biochemistry & Molecular Biology
Yunyun Zhao, Chong Xie, Pei Wang, Zhenxin Gu, Runqiang Yang
Summary: The study showed that GABA can alleviate oxidative damage in soybean sprouts under NaCl stress by enhancing the synthesis of phenolics. Additionally, GABA also increases the activity of antioxidant enzymes and antioxidant capacity, enhancing the resistance of soybean sprouts to stress.
Article
Biochemistry & Molecular Biology
Jianwei Chang, Chong Xie, Pei Wang, Zhenxin Gu, Yongbin Han, Runqiang Yang
Summary: Different lights, especially red light, have significant effects on folate accumulation in wheat seedlings. Red light increases total folate content and the dominant folate component, 5-CH3-THF. Folate content of leaves is higher and more sensitive to light under red light.
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
(2021)
Article
Biochemistry & Molecular Biology
Ge Zhan, Men Long, Kai Shan, Chong Xie, Runqiang Yang
Summary: This study investigated the antioxidant effect of chrysanthemum extract and explored its potential mechanisms through a metabolomics study. The results showed that chrysanthemum extract could alleviate oxidative stress-related cell damage by relieving cell cycle arrest and inhibiting non-apoptotic cell damage. The activities of antioxidant enzymes were also upregulated by chrysanthemum extract. Metabolomics studies revealed that chrysanthemum extract targeted the arginine synthesis pathway and purine metabolism pathway. These findings suggest that chrysanthemum extract has the ability to counteract oxidative stress through multiple pathways and may have potential therapeutic applications.
Article
Agriculture, Multidisciplinary
Chong Xie, Pei Wang, Zhenxin Gu, Runqiang Yang
Summary: Spermidine can alleviate oxidative damage of barley seedlings under UV-B stress and enhance the accumulation of PCs. Exogenous spermidine increased the activities and gene expression of antioxidant enzymes in barley seedlings under UV-B stress. The application of an inhibitor of endogenous spermidine synthesis inhibited the growth and PC accumulation of barley seedlings under UV-B stress, which can be alleviated by exogenous spermidine.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Agriculture, Multidisciplinary
Pei Wang, Guangzheng Wang, Yining Zhang, Xindi Lv, Chong Xie, Juan Shen, Runqiang Yang, Zhenxin Gu, Jianzhong Zhou, Dong Jiang
Summary: Tailormade wheat arabinoxylan (AX) has significant impact on the thermal processing of gluten proteins, promoting their polymerization and unfolding processes, and inducing changes in their molecular structure. The customized AX also enhances the stability of the gluten network and leads to the formation of more homogeneously distributed large protein aggregates.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Chong Xie, Pei Wang, Jianwei Chang, Qiaoe Wang, Yongbin Han, Runqiang Yang
Summary: This study investigated the effects of six amino acids on folate accumulation during wheat germination under red light radiation and established an optimized combination of amino acids for promoting folate enrichment. Applying phenylalanine, tyrosine, tryptophan, glutamate, or p-aminobenzoic acid to wheat seedlings during germination significantly increased the content of total folates. The highest content of folates was observed when the germinated wheat was cultured with a mixture of specific amino acids. This study provides a promising and practical approach to enhance the accumulation of folates in wheat seedlings.
Article
Food Science & Technology
Bailu Yang, Yining Zhang, Jiayi Yuan, Minzhen Yang, Runqiang Yang, Zhenxin Gu, Chong Xie, Qin Zhou, Dong Jiang, Jianzhong Zhou, Pei Wang
Summary: This study investigated the freeze stability of unfermented, pre-fermented, and par-steamed frozen dough in order to advance the industrial production of steamed buns. The results indicated that the best sensory properties of the steamed buns were achieved when using unfermented dough with 2.0% yeast, pre-fermented dough with a pre-fermented time of 30 min, and par-steamed dough with a pre-steamed time of 15 min. The study also revealed the effects of yeast content and fermentation time on the sensory quality of frozen dough.
Article
Plant Sciences
Chong Xie, Maomao Sun, Pei Wang, Runqiang Yang
Summary: This study investigated the effects of GABA and Ca2+ on the biosynthesis of phenolic compounds in soybean sprouts under NaCl stress. Results showed that the addition of Ca2+ increased the content of phenolic compounds, while the addition of LaCl3 inhibited their synthesis. The addition of GABA increased the content of Ca2+ and alleviated the inhibition caused by LaCl3. Both GABA and Ca2+ enhanced the biosynthesis of phenolic compounds and there was an interaction between their effects.
Article
Plant Sciences
Chong Xie, Yahui Zhu, Chaoqun Leng, Qiaoe Wang, Pei Wang, Runqiang Yang
Summary: Spermidine can enhance the biosynthesis of phenolic compounds and alleviate oxidative damage caused by UV-B stress in barley. The application of GABA, the degradation product of spermidine, can further increase the accumulation of phenolic compounds and improve physiological indicators affected by oxidative damage.
Article
Chemistry, Applied
Mian Wang, Yuxuan Ding, Qiaoe Wang, Pei Wang, Yongbin Han, Zhenxin Gu, Runqiang Yang