Cell-Free Systems Based on CHO Cell Lysates: Optimization Strategies, Synthesis of “Difficult-to-Express” Proteins and Future Perspectives

A versatile coupled cell-free transcription-translation system based on tobacco BY-2 cell lysates

(2015) Matthias Buntru et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems

(2015) Anne Zemella et al.   CHEMBIOCHEM

Cotranslational incorporation of non-standard amino acids using cell-free protein synthesis

(2015) Robert B. Quast et al.   FEBS LETTERS

Characterization of trh2 Harbouring Vibrio parahaemolyticus Strains Isolated in Germany

(2015) Silke Bechlars et al.   PLoS One

Synthesis and site-directed fluorescence labeling of azido proteins using eukaryotic cell-free orthogonal translation systems

(2014) Robert B. Quast et al.   ANALYTICAL BIOCHEMISTRY

Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system

(2014) Srujan Kumar Dondapati et al.   BIOSENSORS & BIOELECTRONICS

Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system

(2014) Matthias Buntru et al.   BMC BIOTECHNOLOGY

Wheat germ systems for cell-free protein expression

(2014) Matthias Harbers   FEBS LETTERS

Molecular crowders and cosolutes promote folding cooperativity of RNA under physiological ionic conditions

(2014) C. A. Strulson et al.   RNA

Cell-free synthesis of membrane proteins: Tailored cell models out of microsomes

(2013) Susanne F. Fenz et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Cell-free protein expression based on extracts from CHO cells

(2013) Andreas K. Brödel et al.   BIOTECHNOLOGY AND BIOENGINEERING

Molecular crowding shapes gene expression in synthetic cellular nanosystems

(2013) Cheemeng Tan et al.   Nature Nanotechnology

Quantitative influence of macromolecular crowding on gene regulation kinetics

(2013) M. Tabaka et al.   NUCLEIC ACIDS RESEARCH

IRES-Mediated Translation of Membrane Proteins and Glycoproteins in Eukaryotic Cell-Free Systems

(2013) Andreas K. Brödel et al.   PLoS One

Cell-free synthesis of functional thermostable direct hemolysins of Vibrio parahaemolyticus

(2013) Silke Bechlars et al.   TOXICON

Synthesis of membrane proteins in eukaryotic cell-free systems

(2012) Rita Sachse et al.   ENGINEERING IN LIFE SCIENCES

Comparison of commercial serum-free media for CHO-K1 cell growth and monoclonal antibody production

(2012) Maria Elisa Rodrigues et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system

(2012) Marlitt Stech et al.   JOURNAL OF BIOTECHNOLOGY

CHO cells in biotechnology for production of recombinant proteins: current state and further potential

(2011) Jee Yon Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Biopharmaceutical benchmarks 2010

(2010) Gary Walsh   NATURE BIOTECHNOLOGY

Practical cell-free protein synthesis system using purified wheat embryos

(2010) Kazuyuki Takai et al.   Nature Protocols

Cell-free synthesis and characterization of a novel cytotoxic pierisin-like protein from the cabbage butterfly Pieris rapae

(2010) Joachim H.C. Orth et al.   TOXICON

Computational analysis of membrane proteins: the largest class of drug targets

(2009) Yalini Arinaminpathy et al.   DRUG DISCOVERY TODAY

Mg2+  modulation of EMCV IRES key activity fragment equilibria and r(G·C) base-pair kinetics

(2009) J. A. Dupont et al.   JOURNAL OF BIOLOGICAL PHYSICS

Automated system for high-throughput protein production using the dialysis cell-free method

(2009) Masaaki Aoki et al.   PROTEIN EXPRESSION AND PURIFICATION

Replication of Tomato bushy stunt virus RNA in a plant in vitro system

(2009) Torsten Gursinsky et al.   VIROLOGY